ISSN:
1573-6784
Schlagwort(e):
alginate entrapment
;
covalent immobilization
;
dextransucrase
;
glucooligosaccharides
;
Leuconostoc mesenteroides
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Abstract Dextransucrase from Leuconostoc mesenteroides NRRL B-512F was immobilized using two different methods: covalent attachment to activated silica and entrapment in calcium alginate. For immobilization on silica, native enzyme and dextran-free enzyme were compared. However, the entrapment in calcium alginate beads gave the best results in terms of immobilization yield and stability. This biocatalyst was employed in the acceptor reaction with maltose showing similar glucooligosaccharide production than the native enzyme but increased operational stability.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1008966213425
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