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  • 1
    Electronic Resource
    Electronic Resource
    Cell differentiation Hepatocytes from non-hepatic adult stem cells (2000)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 406 (2000), S. 257-257 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Stem cells are undifferentiated long-lived cells that are capable of many rounds of division. Here we show that adult human liver cells can be derived from stem cells originating in the bone marrow or circulating outside the liver, raising the possibility that blood-system ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35018642
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  • 2
    Electronic Resource
    Electronic Resource
    A new look at epidermal cell kinetics in psoriasis and other dermatoses (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental dermatology 1 (1976), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2230.1976.tb01430.x
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  • 3
    Electronic Resource
    Electronic Resource
    The measurement of the cell cycle time in squamous epithelium using the metaphase arrest technique with vincristine (1977)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 96 (1977), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In squamous epithelia with a single layer of germinative cells, the age distribution of cells in the cell cycle is shown to depend on the direction of the mitotic axis (i.e. a line joining the nuclei of daughter cells) relative to the plane of the basal layer. When axes are in the plane of the basal layer the age distribution is exponential; when cells divide at right angles to the plane of the basal layer, the age distribution is rectangular. When there is a ratio of vertical to horizontal axes, the age distribution is intermediate but can be calculated from knowledge of the proportion of axes in the plane of the layer. Squamous epithelia can be classified according to this arrangement of axes.When there are multiple layers of germinative cells, as in psoriasis, the age distribution is shown to be exponential to a good approximation, whatever the direction of the mitotic axes in the several layers.The importance of these observations is demonstrated by analysing metaphase arrest experiments with vincristine in the single layer of germinative cells in the mouse oesophagus, and in the several layers found in psoriatic epidermis. Choice of the wrong age distribution leads to an error of 6 h in the oesophagus and 23 h in psoriatic epidermis, when the mean cell cycle time is calculated.It is concluded that, in squamous epithelium, it is most important to know the age distribution before calculating the cell cycle time by methods involving measurement of the rate of entry of cells into mitosis or DNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1977.tb07151.x
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  • 4
    Electronic Resource
    Electronic Resource
    The kinetics of metaphase arrest in human psoriatic epidermis: an examination of optimal experimental conditions for determining the birth rate (1981)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 104 (1981), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The optimum experimental conditions have been investigated for the measurement of the birth-rate in human psoriatic epidermis using the vinca alkaloids vinblastine and vincristine. The dose response characteristics of the two drugs were assessed over a 2.5 h collection period; although the dose response characteristics differed between the two agents, a dose of 1μg injected intradermally was considered to be optimal for both. The linearity of metaphase correction was assessed for both agents, taking biopsies for up to 10 h after injection. It was found that there was a delay period for up to an hour which must be avoided during the analysis to prevent an underestimate of the birth rate. However, linearity was then demonstrated for up to 4 h after injection. Metaphase degeneration was assessed by isolating a cohort of unlabelled cells in the G2 phase and tracing their fate over the next 10 h. It was found that the minimum life of an arrested metaphase was of the order of 4 h, and then metaphases degenerated at the rate of approximately 0.4% per hour. It is recommended that metaphase arrest experiments in psoriatic epidermis be confined to 4 h although, if required, correction factors are available to correct for experiments which are lengthened. Finally it is shown that flux into DNA synthesis exceeds the flux into mitosis by four or five times; several explanations are considered, and it is thought that the most likely explanation involves the death of psoriatic cells in the G2 phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1981.tb00943.x
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  • 5
    Electronic Resource
    Electronic Resource
    Induction of a novel epidermal growth factor-secreting cell lineage by mucosal ulceration in human gastrointestinal stem cells (1990)
    [s.l.] : Nature Publishing Group
    Nature 343 (1990), S. 82-85 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Chronic ulceration of the gastrointestinal mucosa is frequent in man, occurring particularly in Crohn's disease and peptic ulcer disease. We have studied freshly-frozen and formalin-fixed tissue from 56 patients with Crohn's disease11 (multiple sites were analysed in ulcerated small intestine ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/343082a0
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  • 6
    Electronic Resource
    Electronic Resource
    The production and characterization of a new monoclonal antibody to the trefoil peptide human spasmolytic polypeptide (1994)
    Springer
    Journal of molecular histology 26 (1994), S. 644-647 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human spasmolytic polypeptide (hSP) is a member of the growing family of trefoil peptides which are expressed in discrete regions of the body, most notably the gastrointestinal tract. Much of the research into the localization of the spasmolytic polypeptide has relied on hybridization in situ to detect its mRNA, due to the absence of a suitable antibody. The aim of the present study was to develop and characterize a monoclonal antibody against the human spasmolytic polypeptide, using a combination of immunohistochemistry and hybridization in situ. After immunoblotting, the antibody detected a 14 kDa protein in gastrointestinal tissue extracts from the stomach and small intestine only. Using immunohistochemistry, human spasmolytic polypeptide showed a distinctive staining pattern in the duodenum which co-localized with its mRNA. The co-localization of the immunoreactive peptide with its mRNA provides good evidence that the antibody truly recognized human spasmolytic polypeptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00158289
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  • 7
    Electronic Resource
    Electronic Resource
    Non-proliferative capacity of endocrine cells of the human gastro-intestinal tract (1996)
    Springer
    Journal of molecular histology 28 (1996), S. 397-398 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02331403
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  • 8
    Electronic Resource
    Electronic Resource
    Failure of induced functional activity and cell deficit to restore preoperative cell number in paired accessory sex glands following unilateral ablation in castrated male mice (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 903-911 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The proliferative response to testosterone propionate in the accessory sex glands of castrated male Balb/c mice has been studied when the glands were intact, and when the glands were unilaterally ablated. The response was analysed by serial measurements of DNA and weight, and by 3H-thymidine labelling techniques. When stimulation is started at three days after castration, most seminal vesicle cells of intact glands carry out DNA synthesis to restore preoperative cell number, whilst the smaller reduction in coagulating gland DNA prior to stimulation results in only about 30% of cells carrying out DNA synthesis to restore former levels. In the unilaterally ablated glands of the 3-day castrates, the cell kinetics of stimulated DNA synthesis and the net accumulation of DNA were similar to the intact glands. A much larger deficit in DNA was apparent in all glands of unstimulated 14-day castrates, resulting in a much larger net accumulation of DNA following testosterone stimulation. No difference between intact and unilaterally ablated glands was detected, and despite prolonged stimulation DNA levels did not exceed preoperative values. Unlike gland DNA levels, gland weights exceeded those of non-castrated mice following prolonged stimulation. However, unilateral ablation did not result in further weight increases in the remaining contralateral glands. It is concluded that the absence of the contralateral sex gland is not detected by the remaining partner during the response to the combined stimuli of a reduction in cell number (resulting from androgen deprivation), and testosterone propionate administration.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930411
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  • 9
    Electronic Resource
    Electronic Resource
    Subcellular distribution of peptides associated with gastric mucosal healing and neoplasia (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 31 (1995), S. 234-247 
    Details
    ISSN: 1059-910X
    Keywords: Immunolocalisation ; Electron microscopy ; Ultrastructure ; hSP ; pS2 ; TFG α ; EGFR ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The trefoil peptides pS2 and human spasmolytic peptide are putative growth factors, particularly associated with mucus-producing cells of the gastrointestinal tract including those of the stomach. The receptor for transforming growth factor alpha (TGF α) takes its name from one of its alternative ligands, epidermal growth factor and is called the epidermal growth factor receptor. Although there is immunoreactive epidermal growth factor in the stomach, it is TGF α and the epidermal growth factor receptor that are abundant. Immunolabelling at electron microscope level allows for subcellular localisation of antigens; pS2 and human spasmolytic peptide co-localise to cytomembranes, including the Golgi apparatus, and thecae of surface/pit mucous cells. TGF α is abundant on the membranes of tubulovesicles of parietal cells and is also present in chief cells: in mucous producing cells it can be detected but not in association with mucous. The distribution of the epidermal growth factor receptor mimics that of TGF α but with preferential clustering on the basolateral membranes of gastric cells. The trefoil peptides are associated with healing and probably act, together with mucus, to protect the gastric mucosa and maintain a viable environment. TGF α, transduced via the epidermal growth factor receptor, inhibits gastric acid secretion, thus aids the trefoils in the maintenance of a gastric microenvironment conducive to healing after damage. TGF α, however, is also a potent mitogen; while this property plays a vital part in repairing mucosal defects, if this peptide or indeed its receptor are overexpressed, the result can be neoplasia. © 1995 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070310307
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