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  • 1
    Electronic Resource
    Electronic Resource
    Involvement of MAP kinase phosphatase-1 in mesangial cell growth regulation (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Nephrology 3 (1997), S. 0 
    Details
    ISSN: 1440-1797
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary: Mitogen-activated protein (MAP) kinase phosphatase-1 (MKP-1) is encoded by the mitogen-inducible gene 3CH 134. MKP-1 has recently been shown to be a dual specificity (serine/threonine, tyrosine) protein phosphatase, which dephosphorylates and inactivates MAP kinases in vitro and in vivo. to seek the role of MKP-1 in growth regulation of mesangial cells, expression of MKP-1 mRNA in cultured mesangial cells and in glomeruli isolated from anti-Thy 1.1 mesangial proliferative glomerulonephritis rats was studied. the effect of inhibition of endogenous MKP-1 by use of antisense-DNA technology on the regulation of MAP kinase activity and the growth regulation of rat cultured inesangial cells was also studied. By northern blot analysis, it was demonstrated that in mesangial cells, MKP-1 mRNA expression was rapidly induced after the stimulation by serum, growth factors and vasoactive peptides. Maximal signals were found in 30-60 min in all growth factors tested. Fetal calf serum (FCS) was the most potent stimulus of MKP-1 mRNA expression, followed by platelet-derived growth factor (PDGF)-B and arginine vasopressin. to elucidate a possible involvement of MKP-1 in disease development of mesangial proliferative glomerulonephritis, MKP-1 mRNA expression was examined in rat anti-Thy 1.1 glomerulonephritis model. A marked increase in MKP-1 mRNA level in isolated glomeruli was observed at day 3 after disease induction (4.3-fold over control). In situ hybridization of MKP-1 mRNA in Thy 1.1 glomerulonephritius rats confirmed the enhanced glomerular expression of MKP-1. to study the role of MKP-1 in mesangial cell growth regulation, phosphorothioate oligodeoxynucleotide (ODN) were used to modulate MKP-1 expression. an antisense ODN targeting the translation initiation site of MKP-1 mRNA inhibited stimulated (by FCS, or PDGF-B) DNA synthesis and FCS- or PDGF-induced mitogenesis in mesangial cells. Sense ODN or mismatched ODN had no effect on the DNA synthesis or mitogenic response of mesangial cells. These results suggest that MKP-1 is an immediately early gene in rat mesangial cells and it plays a critical role in growth regulation of mesangial cells in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1440-1797.1997.tb00268.x
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  • 2
    Electronic Resource
    Electronic Resource
    Tooth mortality and prosthetic treatment patterns in urban and rural Chinese aged 20–80 years (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Community dentistry and oral epidemiology 17 (1989), S. 0 
    Details
    ISSN: 1600-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract The study describes tooth mortality levels and pattern of prosthetic treatments in a sample of 1744 Chinese aged 20–80 yr who are residents of Beijing area. Complete edentulousness was rarely seen before the age of 60 yr. Beyond this age up to 26% were edentulous, depending on age and sex. The mean number of teeth present ranged from 10.0 to 29.7 depending on age, sex, and area of residence. Below the age of 60 yr very few persons had experienced extensive loss of teeth but thereafter the number of teeth missing was substantially increased. Prosthetic treatments in the form of partial dentures, crowns, and bridges were frequently observed even in the younger age groups. In all age groups the number of teeth exhibiting caries lesions involving the pulp was much higher than the number of teeth exhibiting extensive mobility. Although many people retain a high number of teeth even late in life our findings indicate a substantial need for, in particular, relief of pain services. Concurrently, emphasis must be placed on preventive programs aiming at interfering with ongoing disease
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0528.1989.tb00620.x
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic typing of the Senescence-Accelerated Mouse (SAM) strains with microsatellite markers (1999)
    Springer
    Mammalian genome 10 (1999), S. 235-238 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The Senescence-Accelerated Mouse (SAM) strains constitute a murine model of accelerated senescence originating from the ancestral AKR/J strains and consist of nine senescence-prone (SAMP) strains and four senescence-resistant (SAMR) strains. The chromosomes (Chrs) of the SAM strains were typed with 581 microsatellite markers amplified by PCR, and the fundamental genetic information of the SAM strains was obtained. One-third of the examined markers displayed polymorphism among the strains, and only two alleles were detected in almost all loci among the SAM and AKR/J strains. However, in 12 loci (5.6% of total 215 polymorphic markers), the third allele was detected among the SAM strains. The genetic typing and developmental history suggested that the SAM strains were related inbred strains developed by the accidental crossing between the AKR/J strain and other unknown strain(s). Comparison of the distribution of the loci in the SAMP and the SAMR series revealed notable differences in the four regions on Chrs 4, 14, 16, and 17. This indicated that some of these chromosomal sites might contain the genes responsible for accelerated senescence in the SAMP series.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900979
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain (1999)
    Springer
    Mammalian genome 10 (1999), S. 81-87 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F2 intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F2 progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F2 mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2% of the total variance) and 5.8 (10.0%), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F1 and F2. These findings should be useful to elucidate the genetics of osteoporosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900949
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    Paper (German National Licenses)
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