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  • 1
    Electronic Resource
    Electronic Resource
    Determination of six aromatic compounds by using target factor analysis-UV spectrometry (1995)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 351 (1995), S. 325-327 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The UV-spectra solutions containing mixtures of 1-naphthylamine(NAA), 1-naphthol(NAP), 2,7-dihydroxynaphthalene(DHN), 2,4-dimethoxybenzaldehyde(DMO), methyl salicylate(MSA) and dibutyl phthalate (DBP), have been measured. The numbers, identities and concentrations of the aromatic compounds in the mixtures have been determined successfully using Target Factor Analysis. Some sample mixtures have been analyzed successfully. The average recoveries are 99% for NAP, 102% for NAA, 102% for DHN, 103% for DMO, 102% for MSA, 101% for DBP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00321658
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of two chilling-regulated 1-aminocyclopropane- 1-carboxylate synthase genes from citrus (Citrus sinensis Osbeck) fruit (1999)
    Springer
    Plant molecular biology 41 (1999), S. 587-600 
    Details
    ISSN: 1573-5028
    Keywords: ACC synthase ; chilling ; Citrus sinensis ; ethylene ; gene expression ; peel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diurnal change in the temperature below or above 12.5 °C hastens the degreening of citrus peel and elicits the phytohormone ethylene production in citrus fruit. Ethylene triggers the degradation of chlorophyll and synthesis of carotenoids in citrus peel. To investigate if ethylene is required for the degreening of citrus peel elicited by low temperatures, we studied the chilling-regulated gene expression of ACC synthase, one of the key enzymes catalyzing ethylene biosynthesis. We isolated and characterized a chilling-inducible 1-aminocyclopropane-1-carboxylate synthase (ACC synthase) gene, CS-ACS1, and a chilling-repressible gene, CS-ACS2, from citrus peel. The CS-ACS1 transcript 1.7 kb in length encodes a polypeptide of 483 amino acids (M r 54 115, pI 6.63), whereas the CS-ACS2 transcript of 1.8 kb encodes a polypeptide of 477 amino acids (M r 53 291, pI 6.72). Both genes showed a rapid but transient induction (within 2.4 h) of transcripts upon rewarming after the chilling (4 °C) treatment. After 24 h of incubation at room temperature, CS-ACS1 mRNA diminished to an undetectable level, whereas the CS-ACS2 mRNA regained its basal level of expression attained prior to the chilling treatment. Chilling-induced ethylene production and ACC accumulation were also observed upon rewarming. Both genes were also induced by the wound stress (excision). The protein synthesis inhibitor cycloheximide super-enhances the accumulation of both ACS transcripts at room temperature. Molecular analysis of the 3.3 kb genomic DNA of CS-ACS1 revealed that this gene consists of three introns and four exons. The intron 3 is exceptionally large (1.2 kb) and shares significant homology with mitochondrial DNA, supporting the intron-late theory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006369016480
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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