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  • 1
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fullmer's oxytalan fibers are special connective tissue fibers in periodontal ligaments and some non-dental sites of certain animal species, and, ultrastructurally appear to resemble microfibrils related to clastogenesis. The present study has ultrastructurally examined the applicability of Thiéry's periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method for vicinal glycol-containing complex carbohydrates to the study of oxytalan fibers in rat periodontal ligaments and microfibrils of the tunica adventitia in the rat aorta, where microfibrils are often associated with amorphous elastin and are thought to be oxytalan fibers. In the periodontal ligaments, the PA-TCH-SP method weakly to moderately stained collagen fibrils, moderately stained thin fibrils composing the oxytalan fibers, and intensely stained cytoplasmic granules of fibroblasts. In the aortic adventitia, the PA-TCH-SP method moderately stained collagen fibrils. Heavier staining was observed in microfibrils, whereas the amorphous elastin lacked staining. The most intense staining was seen in cytoplasmic granules and glycogen of mural cells. These studies demonstrate that oxytalan fibers in the periodontal ligament of rats and microfibrils in the aorta of rats contain vicinal glycol-containing glycoproteins and the PA-TCH-SP method is a useful tool in ultrastructural studies of oxytalan fibers and microfibrils of rats.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Histopathology 46 (2005), S. 0 
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aims:  To determine the expression of Mcl-1 in testicular germ cell tumours in order to clarify the role of this anti-apoptotic factor in these tumours. Various members of the Bcl-2 family have been implicated in the apoptotic mechanisms regulating germ cell apoptosis. Mcl-1 is an anti-apoptotic Bcl-2 family member and has recently been reported to be related to the progression of malignancy; however, the involvement of Mcl-1 in the development of germ cell tumours is still unknown.Methods and results:  Mcl-1 expression in testicular germ cell tumours was investigated by immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR). By immunohistochemistry, overexpression of Mcl-1 was present in all germ cell tumours that were studied, including embryonal carcinoma and yolk sac tumour, as well as choriocarcinoma and teratoma. In teratomas, Mcl-1 was widely distributed in the epithelial, myogenic, neural and mesenchymal components. RT-PCR analysis after microdissection revealed high levels of Mcl-1 mRNA in all tumour variants compared with non-neoplastic germ cells.Conclusion:  Overexpression of anti-apoptotic Mcl-1 may function to enhance the viability of testicular germ cells, thereby leading to tumorigenesis.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 32 (1996), S. 95-98 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The present study investigated properties of various mixtures of organic acids (malic and malonic) and calcium phosphate compounds (β-tricalcium phosphate, ashed bovine bone, and synthetic hydroxyapatite) with the objective of determining the optimum combination of organic acid and calcium phosphate compound for components of a chitosan-bonded bone-filling paste. β-tricalcium phosphate was decomposed by malic acid and malonic acid, but these two acids did not decompose synthetic hydroxyapatite and ashed bovine bone. Assessment of ion release from a set paste containing either synthetic hydroxyapatite or ashed bovine bone indicated that only calcium ions were appreciably released after storing and stirring the set paste in physiologic saline for 7 days. © 1996 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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