ISSN:
1573-2568
Schlagwort(e):
PANCREATITIS
;
SEPTIC COMPLICATION
;
CYTOKINE-INDUCED NEUTROPHIL
;
CHEMOATTRACTANT
;
MACROPHAGE
;
LUNG COMPLIANCE
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract The role of bronchoalveolar macrophages (BAMs)in the aggravation of cerulein-induced pancreatitis wasstudied by measuring expression of cytokine-inducedneutrophil chemoattractant (CINC) in vitro. Pancreatitis was induced by four intramuscular injections ofcerulein (50 μg/kg at 1-hr intervals). Pancreatitisrats were injected intraperitoneally with 30 mg/kglipopolysaccharide (LPS) 6 hr following the first cerulein injection as a septic challenge. Ratswere divided into four groups: group I, nonpancreatitiswithout LPS; group II, pancreatitis without LPS; groupIII, nonpancreatitis with LPS; and group IV, pancreatitis with LPS. Hyperactivity of BAMs inresponse to LPS was assessed as a function of in vitroCINC production. CINC concentrations of the serum andbronchoalveolar lavage fluid in group IV were significantly higher than those in groups I,II, and III. BAMs in group II harvested 6 hr followingthe first cerulein injection had significantly greaterCINC production than those in group I. Northern blot analysis revealed abundant CINC mRNAtranscripts in BAMs from groups III and IV.Additionally, myeloperoxidase activity in the lung ofgroup IV rats 8 and 12 hr following the first ceruleininjection was significantly higher than that in group I,II, and III rats. Significant differences in static lungcompliance in group IV were found compared with groupsI, II, and III. These results indicate that BAMs from rats with cerulein-inducedpancreatitis were primed and had enhanced release ofCINC following LPS exposure. Enhanced expression of CINCmay modulate the pathogenesis of pancreatitis-associated lung injury complicated with sepsis.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1018809810561
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