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  • 1
    Electronic Resource
    Electronic Resource
    Molecular Phylogenetic Inference of the Woolly Mammoth Mammuthus primigenius, Based on Complete Sequences of Mitochondrial Cytochrome b and 12S Ribosomal RNA Genes (1998)
    Springer
    Journal of molecular evolution 46 (1998), S. 314-326 
    Details
    ISSN: 1432-1432
    Keywords: Key words: Mammoth —Mammuthus primigenius— Proboscidea — Mitochondrial DNA — Molecular phylogeny — Elephant evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Complete sequences of cytochrome b (1,137 bases) and 12S ribosomal RNA (961 bases) genes in mitochondrial DNA were successfully determined from the woolly mammoth (Mammuthus primigenius), African elephant (Loxodonta africana), and Asian elephant (Elephas maximus). From these sequence data, phylogenetic relationships among three genera were examined. Molecular phylogenetic trees reconstructed by the neighbor-joining and the maximum parsimony methods provided an identical topology both for cytochrome b and 12S rRNA genes. These results support the ``Mammuthus-Loxodonta'' clade, which is contrary to some previous morphological reports that Mammuthus is more closely related to Elephas than to Loxodonta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006308
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and chromosomal assignment of a human perforin (PFP) gene (1989)
    Springer
    Immunogenetics 30 (1989), S. 452-457 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed that the human perforin (PFP) gene is located on chromosome17q11-21.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02421177
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  • 3
    Electronic Resource
    Electronic Resource
    Uniparental chromosome elimination in the early embryogenesis of the inviable salmonid hybrids between masu salmon female and rainbow trout male (1997)
    Springer
    Chromosoma 106 (1997), S. 44-52 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Chromosome elimination through chromosome loss and partial deletion is known to be one of the causes of embryonic inviability in some salmonid interspecific hybrids. Using fluorescence in situ hybridization and related techniques, including whole chromosome painting and comparative genomic hybridization, parental origin of eliminated chromosomes was identified in the inviable hybrids between masu salmon (Ms, Oncorhynchus masou) female and rainbow trout (Rb, O. mykiss) male at the early embryonic stage prior to death. In these hybrids, the haploid Rb chromosome number decreased to nearly half, whereas the Ms chromosomes were retained as one or occasionally two full haploid complements. The Rb chromosomes were also involved in the frequently observed fragments and micronuclei. Whereas the occurrence of fragments was constant throughout the observed period, chromosome loss occurred mainly from just after fertilization to the blastulae stage. In tissue sections and cell spreads of late blastula, some Rb chromosomes were trapped in the midzone from ana- to telophase, resulting in micronuclei at the subsequent interphase. Micronuclei and mitotic abnormalities were also observed in the androgenetic haploid hybrids. However, such abnormalities were seldom or never observed in the viable reciprocal hybrids. The present findings suggest that the paternal Rb chromosomes in the inviable hybrids are preferentially eliminated through mitotic abnormalities during early embryogenesis, owing to a possible incompatibility between the maternal Ms cytoplasm and paternal Rb genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050223
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  • 4
    Electronic Resource
    Electronic Resource
    Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain (1990)
    [s.l.] : Nature Publishing Group
    Nature 348 (1990), S. 73-76 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We cloned the XPAC gene, and detected 1.0-1.1 kilobase (kb) XPAC mRNA in mouse cells, and 1.3-1.4 kb and 1.0-1.1 kb XPAC mRNAs in normal human cells1. To obtain human and mouse cDNAs corresponding to these mRNAs, we screened pcD2 human and mouse expression cDNA libraries2 using one of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/348073a0
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  • 5
    Electronic Resource
    Electronic Resource
    Regional assignment of five genes on human chromosome 19 (1987)
    Springer
    Chromosoma 95 (1987), S. 8-12 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A human-mouse hybrid segregant HM76Dd40-6 with new characteristics was derived from the hybrid cell line HM76Dd containing human chromosome 19 as the only human chromosome. Three virus sensitivities located on human chromosome 19 (PVS, E11S and RDRC) were lost in HM76Dd40-6, while six other genes (C3, LDLR, EF2, GPI, PEPD and MANB) were retained. Cytogenetic analysis and in situ hybridization using human or mouse repeated sequences as probes showed that the region q13.1-qter of human chromosome 19 had been replaced by a fragment of mouse chromosome. Our results permit further regional assignment for the following five genes on human chromosome 19: GPI in the region cen-q12, MANB in p13.2-q12, E11S and RDRC in q13.1-qter, and EF2 in pter-q12.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293835
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  • 6
    Electronic Resource
    Electronic Resource
    Quinacrine fluorescence patterns in somatic chromosomes of a t(15q15q) carrier (1972)
    Springer
    Human genetics 〈Berlin〉 15 (1972), S. 66-70 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Eine sporadische Translokation zwischen zwei homologen Chromosomen Nr. 15 wurde mit Hilfe der Quinacrine-Mustard-Fluorescenztechnik bei einem weiblichen Säugling mit Ventrikelseptumdefekt festgestellt, der sonst phänotypisch normal war. Familienuntersuchungen ergaben gewisse individuelle Varianten der Zentromerregion in den Chromosomen 3 und 13, und es wurde eine Vererbung von den Eltern auf ihre Kinder festgestellt. Familienberatungen ergaben gewisse individuelle Variationen der intensiv fluorescierenden Zentromerregion.
    Notes: Summary A sporadic translocation between two homologues of chromosome 15 was identified, by means of the quinacrine mustard fluorescence technique, in a phenotypically normal female infant with ventricular septal defect. Familial studies revealed certain individual variations regarding the intensely fluorescent centromeric regions in chromosomes 3 and 13, which appeared to be transmitted from the parents to offspring.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273432
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  • 7
    Electronic Resource
    Electronic Resource
    Confirmation of the human thymidine kinase locus, 17q21 → 17qter, by means of a man-mouse somatic cell hybrid, D98/AH-2 X LMTK−Cl-1D (1976)
    Springer
    Human genetics 〈Berlin〉 31 (1976), S. 235-239 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A large metacentric marker chromosome, m20, in a line of human D98/AH-2 cells was identified by Q bands as being a translocation (1;17)(p36;q21). This was confirmed by means of somatic cell hybridization between D98/AH-2 and thymidine kinase (TK) deficient mouse cells. The hybrid clones by HAT selective system retained m20, indicating the presence of TK locus on this chromosome. The results also provide evidence that TK gene is located on the distal region of the breakpoint in 17q21 but not on 17q21 → 17pter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296152
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  • 8
    Electronic Resource
    Electronic Resource
    Karyological characterization of a human lymphoblastoid cell line resistant to 6-thioguanine (1977)
    Springer
    Human genetics 〈Berlin〉 35 (1977), S. 201-208 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A mutant human lymphoblastoid cell line, Raji-TG, resistant to 10μg 6-thioguanine (TG)/ml was produced from wild-type cells after exposure to ethylmethane sulfonate. The Raji-TG cells showed their failure to incorporate 3H-hypoxanthine, only 2% as much hypoxanthine guanine phosphoribosyl transferase (HPRT) activity as wild-type cells, and no revertant in HAT selective medium containing hypoxanthine, aminopterin, and thymidine. Raji-TG cells, which were maintained routinely in regular medium lacking TG for as long as 2 years, still retained resistance to the drug and inability to grow in HAT medium. A fusion of Raji-TG cells and mouse cells resistant to 5-bromodeoxyuridine and lacking thymidine kinase formed hybrids, and the resulting hybrid colonies proliferated in HAT medium. These observations strongly supported the hypothesis that Raji-TG line cells might be originated from a mutational event with deficiency of HPRT. Both parental and the mutant have a modal chromosome number of 49 with a remarkably stable karyotype. Excess chromosome materials are found in chromosomes 1, 5, 7, 14, and 16. Chromosome 8 is completely missing, but is represented by two respective isochromosomes of the short and long arms of No. 8. Five different marker chromosomes could be distinguished, and most of their origin has been determined. Isolation of Raji-TG X mouse hybrid clones which contained one of each marker chromosome is of considerable value in mapping human genes on regions within particular chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393971
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  • 9
    Electronic Resource
    Electronic Resource
    Euploid somatic recombinants with two active X or XY1Y2 chromosomes isolated from cultured male Indian muntjac cells after HVJ virus fusion, and their use for gene assignment (1978)
    Springer
    Somatic cell and molecular genetics 4 (1978), S. 437-450 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Four diploid somatic recombinants were isolated from hybrids either between or within two diploid cell lines of a male Indian muntjac after HVJ virus-mediated cell fusion. Both parental lines had a normal male karyotype, 7,XY1Y2, in which the largest autosomal pair was heteromorphic with respect to the size of the secondary constriction (1h+/1h−), C bands, and nucleolar organizers. Of the four recombinants, three showed a 6,XX,1h+/1h+ or 1h+/1h− karyotype, the remaining one a 7,XY1Y2,1h+/1h+. No late-replicating X chromosome was found in the XX recombinants, although it was demonstrated in the natural XX line, suggesting the presence of two active X chromosomes in the former. The G6PD, PGK, and HPRT activities were proportional to the number of active X chromosomes present in all cell types examined, indicating their X-linkage, whereas the same level of activities obtained for LDH and 6PGD indicated their autosomal linkage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538865
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  • 10
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA analysis of mouse-rat hybrid cells: Effect of chloramphenicol selection on the relative amounts of parental mitochondrial DNAs (1982)
    Springer
    Somatic cell and molecular genetics 8 (1982), S. 67-81 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Several mouse-rat somatic hybrid cell lines were isolated by fusing chloramphenicol-resistant (CAPR) and CAP-sensitive (CAPS) parent cells, and propagation of the parent mitochondrial DNA (mtDNA) species in the hybrid cells was studied. The restriction endonucleases EcoRI, HpaII, and HaeIII were used for identification of mtDNA species. Both mouse and rat mtDNAs were propagated in all the hybrid cells examined and maintained during long-term cultivation and repeated cell division. Moreover, in CAPR mouse-rat hybrid cells, selection and successive cultivation in the presence of CAP did not increase the relative amount of mtDNA species of CAPR parent cell origin, and when CAP was removed from the culture medium, mtDNA species of CAPR parent cell origin did not decrease appreciably. The amount of mouse mtDNAs was consistently 1–4 times that of rat mtDNAs in the mouse-rat hybrid cells regardless of the species of parent cells from which the CAP resistance was derived. Thus mouse-rat hybrid cells have a stable mtDNA population in which the amount of mouse mtDNAs is larger than that of rat mtDNAs without any influence of CAP selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538651
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