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  • 1
    Electronic Resource
    Electronic Resource
    Current applications of single-cell PCR (2000)
    Springer
    Cellular and molecular life sciences 57 (2000), S. 96-105 
    Details
    ISSN: 1420-9071
    Keywords: Key words. PCR; single cells; diagnosis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The advent of the polymerase chain reaction (PCR) has revolutionised the way in which molecular biologists view their task at hand, for it is now possible to amplify and examine minute quantities of rare genetic material: the limit of this exploration being the single cell. It is especially in the field of prenatal diagnostics that this ability has been readily seized upon, as it has opened up the prospect of preimplantation genetic analysis and the use of fetal cells enriched from the blood of pregnant women for the assessment of single-gene Mendelian disorders. However, apart from diagnostic applications, single-cell PCR has proven to be of enormous use to basic scientists, addressing diverse immunological, neurological and developmental questions, where both the genome but also messenger RNA expression patterns were examined. Furthermore, recent advances, such as optimised whole genome amplification (WGA) procedures, single-cell complementary DNA arrays and perhaps even single-cell comparative genomic hybridisation will ensure that the genetic analysis of single cells will become common practice, thereby opening up new possibilities for diagnosis and research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050501
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of tumor cells in leukapheresis products from patients with breast cancer using immunocytochemical staining method (2000)
    Springer
    Archives of gynecology and obstetrics 263 (2000), S. 119-125 
    Details
    ISSN: 1432-0711
    Keywords: Key words Breast cancer ; Immunocytochemistry ; Tumor cell detection ; Leukapheresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We used a combination of 4 monoclonal antibodies (BM7, BM8 against MUC1, 5D3 aganist CK8,18,19 and HEA125 against human epithelial antigen) and a sensitive immunocytochemical staining using cytospin preparation to identify breast tumor cells in leukapheresis products (LP). This assay allowed detection of one tumor cell in 1×106 mononuclear cells (MC). In clinical specimens, tumor cells were detected in LP from 6 of 42 (14.3%) patients in the adjuvant treatment group, from 2 of 11 (18.2%) patients in the neoadjuvant treatment group and from 9 of 43 (20.1%) in the group of patients with metastatic disease. Tumor cell counts ranged from 0.25–5 cells in 1×106 normal cells per LP. The median tumor cell concentration was higher in specimens from patients with metastatic disease (median=0.96) than in specimens from patients in the adjuvant and neoadjuvant treament groups (median=0.5 and 0.75). No significant differences between the epithelial cell positive group and the epithelial cell negative group with respect to tumor size, lymph nodes involvement, tumor grade, histological type and receptor were found. We conclude that immunocytochemical staining of cytospin preparation is a sensitive and simple method to detect and quantitate breast cancer cells in LP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004040050009
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Evaluating GA733-2 mRNA as a marker for the detection of micrometastatic breast cancer in peripheral blood and bone marrow (1999)
    Springer
    Archives of gynecology and obstetrics 263 (1999), S. 2-6 
    Details
    ISSN: 1432-0711
    Keywords: Key words Expression ; GA733-2 ; RT-PCR ; Bone marrow ; Peripheral blood
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The GA733-2 gene encodes the epithelial glycoprotein 40, a homophilic cell-cell adhesion molecule, which is expressed on the surface of epithelial cells and associated with a variety of carcinomas, e.g. breast, colorectal and lung carcinomas. To test if it could serve as a tumor marker, we have analysed the expression of GA733-2 in bone marrow (BM) and peripheral blood (PB) from healthy donors, and of patients with haematological malignancies or breast cancer using reverse transcriptase-polymerase chain reaction (RT-PCR). The GA733-2 nested PCR was positive in 100% (8/8) of BM and 40% (16/40) of PB from healthy donors, in 100% (33/33) of BM from patients with breast cancer who had no evidence of distant metastases and also in BM and PB from patients with haematological malignancies. GA733-2 mRNA is not specific as a marker for the detection of breast cancer cells in BM and PB.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004040050251
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    Paper (German National Licenses)
    Fulltext
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