ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Effects of UVB irradiation on keratinocyte growth factor (KGF) and receptor (KGFR) expression in cultured human keratinocytes (1996)

Zhou, Youwen ; Lee, Hyung-Suk T. ; Kooshesh, Fatemeh ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Experimental dermatology 5 (1996), S. 0

add to mindlist on the mindlist

Details

ISSN: 1600-0625

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract Keratinocyte growth factor (KGF) and its receptor (KGFR) are thought to play important roles in normal keratinocyte growth and differentiation. Since UVB radiation is known to influence keratinocyte growth, we sought to determine whether UVB would alter the expression of KGF and KGFR. Using a reverse-transcription coupled polymerase chain reaction (RT-PCR). the present study examined the expression of KGF and KGFR mRNA in cultured normal human keratinocytes exposed to UVB irradiation. Total cellular RNA was extracted from cultured keratinocytes at various time points after irradiation, reverse transcribed and used for PCR amplification using primers specific for KGF and KGFR. Constitutive expression of KGFR mRNA, but not KGF mRNA, was detected in normal cultured human keratinocytes. After UVB irradiation at 300 J/m2, the KGF mRNA remained undelectable while the KGFR mRNA level was significantly decreased. The downregulation of KGFR mRNA expression was also confirmed by Northern blot analysis. Immunohistochemical studies demonstrated a decreased positive signal of KGFR in human keratinocytes after UVB irradiation. Our results suggest a possible role for the KGF-KGFR signalling pathway in the skin after exposure to UVB, and that UVB-induced growth inhibition of keratinocytes in hyperproliferative skin disorders may be related to downregulation of KGFR.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1600-0625.1996.tb00108.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Expression of the UNC-5 guidance receptor in the touch neurons of C. elegans steers their axons dorsally (1993)

Hamelin, Michel ; Zhou, Youwen ; Su, Ming-Wan ; [et al.]

[s.l.] : Nature Publishing Group

Nature 364 (1993), S. 327-330

add to mindlist on the mindlist

Details

ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] A lacZ reporter fused to the 5'-flanking sequence of the mec-7 gene is expressed specifically and abundantly in the six touch receptor neurons7. We used the same mec-7 regulatory sequences to drive unc-5 expression in these cells. The touch receptor neurons have appropriate trajectories for ...