ISSN:
1476-5535
Schlagwort(e):
Keywords: enzyme production; fed-batch fermentation; β-galactosidase; lactase; Streptococcus salivarius subsp thermophilus
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
The production of β-galactosidase by an autolytic strain of Streptococcus salivarius subsp thermophilus 11F was investigated in batch and fed-batch 2-L working volume stirred tank bioreactors. β-Galactosidase was released into the medium upon cell lysis within 1–2 h after the maximum biomass quantity was reached. In batch fermentations the highest β-galactosidase activity of 69 U ml−1 was obtained when the temperature was increased to 42°C after a 4-h growth period at 30°C. In fed-batch experiments the highest β-galactosidase activity of 74 U ml−1 was obtained at a constant 37°C.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1038/sj.jim.2900515
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