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A method of direct-viewing comparative analysis of tumour cell motility in vitro; effect of pH and adhesion on cells of different malignancy (1998)

Pavlíková, L. ; Zicha, D. ; Chaloupková, A. ; [et al.]

Springer

Experimental biology online 3 (1998), S. 1-13

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ISSN: 1430-3418

Keywords: Motility assay ; Stress conditions ; Tumour cells

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A novel method for the comparative analysis of cell motility by direct viewing was developed and used in a preliminary study of sarcoma cells. Three cell lines from the RPS family of sarcomas in inbred LEW/CUB rats which differ in the incidence of spontaneous metastasis were studied. A system of four different culture conditions, designed to mimic the stress within the tumour, was created by changing and combining two variables: the pH of the medium (with 2% calf serum only), which was either physiological at 7.4 or 6.6; and the adhesiveness of the culture substratum, which was either at a standard level or decreased. It was found that changing the pH from 7.4 to 6.6 in a single step slowed the cells down, and also changed the way in which they moved, from «walking« in random directions to moving in a more directional way. Making the culture surface less adhesive sped up cell locomotion. Decreasing the adhesiveness of the culture substratum and the pH simultaneously stimulated the migration of highly metastasizing cells preferentially. Thus we have found a way to distinguish between highly metastasizing A297Nb sarcoma cells and poorly metastasizing T15 and non-metastasizing K2 sarcoma cells, a distinction that could not be made by a simple examination of the cells. It is concluded that a comparison of cell motility by directly viewing the cells under different conditions may be useful when investigating the in vitro motility properties of malignant cells.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s00898-998-0008-0

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Actin cytoskeleton and motility in rat sarcoma cell populations with different metastatic potential (1994)

Pokorná, E. ; Jordan, P. W. ; O'Neill, C. H. ; [et al.]

New York, NY : Wiley-Blackwell

Cell Motility and the Cytoskeleton 28 (1994), S. 25-33

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ISSN: 0886-1544

Keywords: actin structures ; vinculin ; focal contacts ; spontaneous metastasis ; extracellular pH ; cell motility ; malignancy ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: We have studied the organisation of the actin cytoskeleton in three related rat sarcoma cell populations of differing malignancy. They were derived by neoplastic progression from a population which had transformed spontaneously in vitro, and were distinguished by their ability to give rise to reproducibly different numbers of metastases, ranging from 10% to 80% of the animals inoculated. We found characteristic differences in the arrangement of the actin cytoskeleton. Confocal three-dimensional microscopy showed that nearly all of the least malignant population contained conspicuous actin stress fibres lying in the lower part of the cell parallel to the substratum and no other actin structures. Actin in the intermediate population was typically situated in a diffuse layer underlying the whole plasma membrane, in which no fibres could be seen. Two thirds of the most malignant population consisted of more rounded cells filled with a three-dimensional network of fine oblique actin fibres. There were focal contacts in all these cells; their area showed a regular decrease from 1.3 μm2 to 0.4 μm2. The differences in actin distribution were accompanied by differences in motility, which increased as malignancy increased. When individual cells were fixed after they had been tracked by time-lapse, their cytoskeleton type correlated with the speed at which they had moved. All these differences were enhanced at low pH. These findings point to the possibility that the three-dimensional network of fine actin fibres in acid culture could be a measure of the malignant potential of transformed cells in vitro. © 1994 Wiley-Liss, Inc.

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