ISSN:
1573-8469
Keywords:
actin
;
fungal biomass
;
grey mould
;
necrosis
;
PR proteins
;
β-tubulin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract An inoculation procedure was developed to obtain efficient and synchronous infection on detached tomato leaves by Botrytis cinerea. In spray-inoculated leaves incubated at 20 °C, the infection process consisted of three phases: the formation of primary necrotic lesions (until 20 hpi), a quiescent phase (20-72 hpi), and the expansion of a proportion of the primary lesions (from 72 hpi onwards), resulting in full tissue maceration. At 4 °C, the infection progressed slowly but steadily without inducing necrotic responses in the host. The actin and β-tubulin genes of B. cinerea were cloned, characterized and used as probes on blots containing RNAs from leaves at various stages of the infection. The genes displayed a similar expression pattern throughout the infection and the hybridization signal reflected the amount of fungal biomass. The actin mRNA accumulated to higher levels than the β-tubulin mRNA. Tomato PR protein mRNAs (chitinase, β-1,3-glucanase and PR-1) were induced during the infection, albeit with different kinetics and to different levels. At 20 °C, β-1,3-glucanase and PR-1 mRNAs were induced more rapidly than chitinase mRNAs. At 4 °C, mRNAs encoding extracellular β-1,3-glucanase and intracellular, as well as extracellular chitinase were hardly induced.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008698116106
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