ISSN:
1432-1106
Schlagwort(e):
Neurotensin projection
;
Lateral parabrachial nucleus
;
Central amygdaloid nucleus
;
Coexistence
;
Calcitionin gene-related peptide
;
Immunocytochemistry
;
Double-staining method
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary The origin of neurotensin-like immunoreactive (NTI) fibers in the central amygdaloid nucleus (AC) in the rat was examined using indirect immunofluorescence and retrograde tracing combined with immunocytochemistry. Destruction of the external subdivision of the lateral parabrachial nucleus, which contains a group of NTI neurons, resulted in a marked reduction of these fibers in the ipsilateral AC, which suggests that most of these fibers are of extrinsic origin. This was also supported by the finding that injection of fast blue dye into the AC labeled many neurons in the external subdivision of the lateral parabrachial nucleus ipsilaterally, and that simultaneous treatment with antiserum against NT stained some of these neurons. Subsequent immunohistochemical staining of alternate sections revealed that many of these NTI neurons were also labeled by calcitonin gene-related peptide antiserum.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00248753
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