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  • 1
    Electronic Resource
    Electronic Resource
    The presence of a calcitonin gene-related peptide in the olivocochlear bundle in rat (1986)
    Springer
    Experimental brain research 61 (1986), S. 575-578 
    Details
    ISSN: 1432-1106
    Keywords: Calcitonin gene-related peptide ; Olivocochlear bundle ; Retrograde axonal transport ; Immunohistochemistry ; Biotin-wheat germ agglutinin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The origins of calcitonin gene-related peptide immunoreactive (CGRPI) fibers in the cochlea were examined in rats. Parasagittal transection of the brain just medial to the principal sensory trigeminal nucleus resulted in the ipsilateral disappearance of CGRPI fibers in the cochlea, indicating that the origins of these fibers lie in the central nervous system. Next, we used a highly sensitive method combining retrograde tracing and immunohistochemistry to identify the origins of the CGRPI fibers in the cochlea. After injection of biotin-wheat germ agglutinin (b-WGA) into the cochlea, CGRPI neurons in the ipsilateral lateral superior olivary nucleus also contained b-WGA granules. These findings indicated tht CGRPI efferent fibers are major components of the olivocochlear bundle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237583
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution and origin of calcitonin gene-related peptide in the rat stomach and duodenum: An immunocytochemical analysis (1987)
    Springer
    Journal of neural transmission 68 (1987), S. 1-14 
    Details
    ISSN: 1435-1463
    Keywords: CGRP ; stomach ; duodenum ; distribution ; origin, rat ; immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We studied the three-dimensional distribution of structures with calcitonin gene-related peptide-like immunoreactivity (CGRPI) in the rat stomach and duodenum, including the origins of these structures, using indirect immunofluorescence in both muscle strips and frozen sections. There was a very dense meshwork of CGRPI fibers in the circular and longitudinal muscle layers, and also in the myenteric and submucous plexuses of the stomach and duodenum. No CGRPI neurons were seen in the stomach, even in rats treated with colchicine; in the duodenum, there was a group of CGRPI cells in the myenteric and submucous ganglia. No regional differences were seen in the stomach and duodenum. We found by experimental manipulations that CGRPI fibers in the stomach were exclusively extrinsic in origin; some of such fibers in the duodenum were intrinsic in origin, though most were supplied by CGRPI cells outside the duodenum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244635
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Projection of neurotensin-like immunoreactive neurons from the lateral parabrachial area to the central amygdaloid nucleus of the rat with reference to the coexistence with calcitonin gene-related peptide (1988)
    Springer
    Experimental brain research 71 (1988), S. 603-610 
    Details
    ISSN: 1432-1106
    Keywords: Neurotensin projection ; Lateral parabrachial nucleus ; Central amygdaloid nucleus ; Coexistence ; Calcitionin gene-related peptide ; Immunocytochemistry ; Double-staining method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The origin of neurotensin-like immunoreactive (NTI) fibers in the central amygdaloid nucleus (AC) in the rat was examined using indirect immunofluorescence and retrograde tracing combined with immunocytochemistry. Destruction of the external subdivision of the lateral parabrachial nucleus, which contains a group of NTI neurons, resulted in a marked reduction of these fibers in the ipsilateral AC, which suggests that most of these fibers are of extrinsic origin. This was also supported by the finding that injection of fast blue dye into the AC labeled many neurons in the external subdivision of the lateral parabrachial nucleus ipsilaterally, and that simultaneous treatment with antiserum against NT stained some of these neurons. Subsequent immunohistochemical staining of alternate sections revealed that many of these NTI neurons were also labeled by calcitonin gene-related peptide antiserum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248753
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Ontogeny of calcitonin gene-related peptide and calcitonin in the rat thyroid (1986)
    Springer
    Histochemistry and cell biology 84 (1986), S. 139-143 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this immunohistochemical study, the ontogenic development of calcitonin-gene-related peptide (CGRP) in the rat thyroid was investigated and compared with that of calcitonin using the indirect-immunofluorescence method. Parafollicular cells with immunoreactivity to both CGRP and calcitonin first appeared at an early stage of gestation (days 17 and 18) in the central portion of the thyroid. Cells immunoreactive to CGRP and calcitonin had became numerous by gestational day 22. After postnatal day 7, CGRP- and calcitonin-immunoreactive (CIR) cells increased rapidly both in number and in the intensity of their fluorescence. In 14- to 90-day old rats, many intensely immunoreactive cells were distributed in the central portion of the thyroid. The cells immunoreactive to CGRP and to calcitonin had an almost identical ontogenic appearance. In 14-day-old and adult rats, C-IR cells also exhibited CGRP immunostaining, suggesting that these cells simultaneously produce and store CGRP during ontogeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499824
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    Paper (German National Licenses)
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