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  • 1
    Digitale Medien
    Digitale Medien
    Altered development of polysomal RNA activity in chick-pea (Cicer arietinum L.) embryonic axes. Effects of abscisic acid and temperature (1985)
    Springer
    Planta 164 (1985), S. 517-523 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Abscisic acid and RNA translation ; Cicer ; Embryonic axis ; Germination and temperature (seeds) ; Poly (A)RNA ; Protein synthesis ; Temperature and RNA translation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The in vitro activity of polysomal polyadenylated RNA (poly(A)RNA) was studied using chick-pea (Cicer arietinum L.) embryonic axes subjected to treatments retarding germination (H2O 30°C and abscisic acid [ABA] 30°C) or inducing a false germination (thiourea 30°C) in which normal protein synthesis and growth did not occur. All treatments induced a smaller proportion of poly(A)RNA compared with the control (H2O 25°C). However, poly(A)RNA obtained in the presence of ABA had a similar in vitro activity to that of the control. The translation of mRNA from embryonic axes germinated at high temperatures was extensively blocked (70%) by methyl-7-guanosine-5′-triphosphate, whereas mRNA translation from axes treated with H2O-25°C and ABA was completely blocked (100%), indicating a greater cap dependence in the latter cases. Polyacrylamide gel electrophoresis showed that ABA and H2O-30°C each induced the synthesis of a polypeptide with an approximate Mr of 32 kDa, probably a germination regulator. It is suggested that ABA and high temperatures could regulate germination at the translational level as well as affecting ionic-exchange properties, as has been previously demonstrated (Hernández-Nistal et al. 1983, Physiol. Plant. 57, 273–278).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00395969
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Italian population data on the loci MR, GYPA, HBGG, D7S8 and GC (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 161-162 
    Details
    ISSN: 1437-1596
    Schlagwort(e): PM loci (LDLR, GYPA, HBGG, D7S8, GC) ; PCR ; DNA polymorphism ; Population genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract Allele and genotype frequencies for the five loci LDLR, GYPA, HBGG, D7S8 and GC were determined for 374 unrelated Italians using a multiplex PCR-amplification and typing commercial kit. The distribution of the genotype frequencies showed no deviations from Hardy-Weinberg expectations. The combined power of discrimination and chance of exclusion for all five loci were 0.999 and 0.702, respectively. A test for homogeneity was performed and no significant differences were observed among the Caucasian population samples.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01369681
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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