Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Fluorescence in-situ hybridization (FISH) reveals that in chronic myelogenous leukaemia (CML) following interferon-α therapy, normalization of megakaryocyte size is associated with the loss of bcr/abl translocation (1997)
    Oxford, UK : Blackwell Science Ltd
    Histopathology 31 (1997), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In addition to predominant granulocytic proliferation, bone marrow morphology in Philadelphia chromosome positive (Ph1+) CML is characterized by atypical dwarf or microforms of megakaryocytes. However, following therapy with interferon-α2b (IFN), these micromegakaryocytes occur less frequently. The purpose of this study was to elucidate whether the reappearance of normal megakaryocytes may be associated also with a reduction of the bcr/abl-positive cell clone.〈section xml:id="abs1-2"〉〈title type="main"〉Methods and results:Fluorescence in-situ hybridization (FISH) technique in combination with immunomorphometry (CD61) was performed on trephine biopsies. A total of 311 CD61-positive megakaryocytes, including precursors and atypical microforms, were evaluated in pre-treatment specimens derived from 11 patients with Ph1+ CML. A specific fusion site marking the bcr/abl translocation was found in 87% of megakaryocytes which showed a size of 169 ± 35 μm2. In untreated patients, atypical microforms (size 200 μm2) were observed in 66% of the total megakaryocytic population. Following IFN therapy 369 megakaryocytes could be analysed in sequential examinations and were found to display a significant decrease (63%) in positive fusion signals. In addition there was also a significant enhancement in average size (252 ± 66 μm2) reflecting a reduction in the number of micromegakaryocytes (43%). These findings were particularly conspicuous in three patients with a major to complete cytogenetic remission.〈section xml:id="abs1-3"〉〈title type="main"〉Conclusions:A normalization of megakaryocyte size following IFN therapy in CML is significantly associated with a loss of the bcr/abl translocation site and therefore indicates a (partial) recovery of normal haematopoiesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.1997.2480853.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Changing patterns of histological subgroups during therapy of Ph1+ chronic myelogenous leukaemia (2000)
    Oxford, UK : Blackwell Science Ltd
    Histopathology 37 (2000), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: AimsBone marrow histopathology reveals a striking heterogeneity at diagnosis of Philadelphia chromosome positive (Ph1+) chronic myelogenous leukaemia (CML). Based on semiquantitative evaluations of the number of megakaryocytes and the content of fibres, various histological subtypes have been postulated. However, little information exists on whether these groups represent stable categories of the different classification systems and whether therapeutic regimes exert any influence on the putative shift of histological patterns.Methods and resultsA retrospective clinicopathological study was performed on 396 bone marrow biopsies derived from 173 patients. There were at least two representative trephines taken at diagnosis and at median intervals of 16 months. Processing of the specimens involved immunostaining with CD61 (megakaryopoiesis) and Ret40f (erythropoiesis) and Gomori's silver impregnation technique. Based on morphometric analysis and in accordance with the general appearance of bone marrow histology three different histological subtypes were distinguished. These consisted of a granulocytic (51 patients), a predominantly megakaryocytic (73 patients) and a myelofibrotic pattern (49 patients). Follow-up biopsies revealed that a significant transition of histological groups occurred and that, independently of treatment modalities, the myelofibrotic category was associated with an unfavourable prognosis. Of the 124 patients without myelofibrosis at onset, 42% later transformed into the myelofibrotic subtype. However, these patients showed no prevalence of either a pre-existing granulocytic or megakaryocytic growth. Myelofibrotic changes were significantly associated with interferon (IFN) and busulfan (BU) therapy. On the other hand, a transition of a myelofibrotic into a nonfibrotic subtype was detectable in 17 of the 49 patients under study and related to hydroxyurea (HU) treatment.ConclusionsHistological classification systems of bone marrow features in CML do not represent stable patterns, but may be significantly altered by therapy, in particular IFN and HU.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.2000.00993.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    267. Herkunft und prospektive Potenz der Zellen des Makrophagensystems (1974)
    Springer
    Langenbeck's archives of surgery 337 (1974), S. 886-886 
    Details
    ISSN: 1435-2451
    Keywords: Macrophages ; Monocytes ; Reticulum Cells ; Fibroblasts ; Makrophagen ; Monocyten ; Reticulumzellen ; Fibroblasten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mittelpunkt des Makrophagensystems rind die Monocyten. Sic bilden sich im Knochenmark aus Promyelocyten iiber Promonocyten. Nach Ausschwemmung ins Blut verlassen sic die Gefäße and werden extravasal zu Makrophagen verschiedener Gestalt (Fettkörnchenzellen, Alveolar- and Peritonealmakrophagen, Lipophagen, Kupffer-Zellen, phagocytierende Reticulumzellen der hämopoietischen Organe etc.). Auch Epitheloidzellen und reaktive Riesenzellen sind Monocytenderivate. Ferner können rich Endothelzellen, Fibroblasten und Gewebsmastzellen aus Monocyten entwickeln.
    Notes: Summary Monocytes are the basic cell form of the macrophage system. They originate in the bone marrow from promyelocytes, passing through the stage of promonocytes. After reaching the blood they leave the vessels and turn into various types of macrophage (gitter cells, alveolar and peritoneal macrophages, lipophages, Kupffer cells, phagocytic reticulum cells of the hemopoietic organs, etc.). Epithelioid cells and reactive giant cells are further derivatives of monocytes. Endothelial cells, fibroblasts, and tissue mast cells can also develop from monocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01278900
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Die Entstehung von Gewebsmastzellen bei Ratten (1972)
    Springer
    Research in experimental medicine 157 (1972), S. 198-201 
    Details
    ISSN: 1433-8580
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851136
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Macrophages and their subpopulations following allogeneic bone marrow transplantation for chronic myeloid leukaemia (2000)
    Springer
    Virchows Archiv 437 (2000), S. 160-166 
    Details
    ISSN: 1432-2307
    Keywords: Key words Macrophages ; Pseudo-Gaucher cells ; Chronic myeloid leukaemia ; Bone marrow transplantation ; Bone marrow biopsies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  A morphometric and immunohistochemical study was performed on 354 bone marrow trephine biopsies derived from 126 patients with chronic myeloid leukaemia (CML) before and after allogeneic bone marrow transplantation (BMT). The purpose of this investigation was to evaluate the macrophage population, including several subsets and their dynamics in the posttransplant period. In addition to the total CD68+ resident (mature) macrophages the so-called activated fraction identified by its capacity to express α-d-galactosyl residues, the pseudo-Gaucher cells (PGCs) and the iron-laden histiocytic reticular cells were also considered. Following immuno- and lectin-histochemical staining morphometric analysis was carried out on sequential postgraft bone marrow specimens at standardized intervals. Compared to the normal bone marrow and calculated per haematopoiesis (cellularity) an overall decrease of about 40–50% in the quantity of CD68+ macrophages and the BSA-I+ subpopulation was detectable in the early posttransplant period (9–45 days after BMT). Noteworthy was the temporal recurrence of PGCs in the engrafted bone marrow, which was not associated with a clonally transformed cell population or leukaemic relapse. Reappearance of postgraft PGCs was most prominent in the first 2 months after BMT. This conspicuous feature was presumed to be functionally associated with a pronounced degradation of cell debris following pretransplant myelo-ablative therapy (scavenger macrophages). Evidence for an activation of the BSA-I+ macrophage subset was derived from the identical carbohydrate-binding capacity shown by the PGCs. In the regenerating haematopoiesis shortly after BMT a significant correlation between the number of BSA-I+ macrophages and erythroid precursor cells was determinable. This result implicates a close functional relationship between postgraft reconstitution of erythropoietic islets and centrally localized activated macrophages. In conclusion, findings emerging from this study included the reappearance of PCGs in the engrafted bone marrow independently of a leukaemic relapse and the significant association of the activated BSA-I+ macrophage subset with the recovery of erythropoiesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280000224
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Morphologie eines malignen Parotislymphoms mit Paraproteineinschlüssen (1978)
    Springer
    European archives of oto-rhino-laryngology and head & neck 219 (1978), S. 391-392 
    Details
    ISSN: 1434-4726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A female patient presented with a lump in the right parotedeal region. A needle aspiration biopsy was performed which revealed numerous monomorphous atypical lymphoid cells with PAS-negative cytoplasmic inclusions. This raised the suspicion of malignancy. Therefore, the tumour was excised. Histologically, a nodular lymphoma of low grade of malignancy was diagnosed. The tumour contained many epithelioid histiocytes, sometimes with PAS-positive material. The case was difficult to classify after the Kiel-nomenclature, whilst classification on the basis of Rappaport's system was much easier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00463843
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Die fermentcytochemische Erkennung normaler und neoplastischer Erythropoesezellen in Schnitt und Ausstrich (1967)
    Springer
    Annals of hematology 15 (1967), S. 289-293 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Reifere Erythropoesezellen enthalten charakteristischerweise perinukleär α-Naphthylacetat-Esterase und jüngere paranukleär saure Phosphatase. Diese Eigenschaften können zur Diagnostik von neoplastischen Erkrankungen der Erythropoese herangezogen werden. Dies wird an Hand von drei einschlägigen Beobachtungen dargelegt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01632381
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Diagnostic experiences with the naphthol AS-D chloroacetate esterase reaction (1970)
    Springer
    Annals of hematology 21 (1970), S. 1-8 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Hand von einschlägigen Beispielen werden einige mit der Naphthol-AS-D-Chloracetat-Esterase-Reaktion erhobene Befunde mitgeteilt und ihre diagnostischen Anwendungsmöglichkeiten besprochen. Bei der Untersuchung von 140 Hämoblastosen zeigte es sich, daß ein beträchtlicher Teil der myeloischen Hämoblastosen einen sog. Naphthol-AS-D-Chloracetat-Esterase-Defekt der reifen neutrophilen Granulozyten aufweist. Damit spricht ein solcher Defekt in Zweifelsfällen für eine myeloische und gegen eine lymphatische Erkrankung des blutbildenden Systems. Auch der Nachweis von Pseudopelger-Zellen kann in diesem Sinne gewertet werden, da sie zuweilen Naphthol-AS-D-Chloracetat-Esterase-positive Auerstäbchen enthalten, wodurch sie in solchen Fällen als leukämische Zellen ausgewiesen sind. Ferner können sehr unreifzellige Hämoblastosen schon auf Grund weniger Naphthol-AS-D-Chloracetat-Esterase-positiver Blasten als myeloisch aufgefaßt werden. Schließlich wird darauf hingewiesen, daß in seltenen Leukosefällen auch einmal die Eosinophilen entgegen der Norm Naphthol-AS-D-Chloracetat-Esterase-Aktivität enthalten können.
    Notes: Summary Some observations with the naphthol AS-D chloroacetate esterase reaction are reported and their diagnostic significance is discussed. This is illustrated by some characteristic cases. By means of cytochemical investigations on 140 cases of various hemoblastoses a so-called naphthol AS-D chloroacetate esterase defect could be detected in a considerable number of the myeloid cases. Therefore, the demonstration of such an enzyme defect in diagnostic doubtful cases suggests a myeloid rather than a lymphatic disease of the blood forming system. The occurence of pseudo-Pelger cells can be interpreted in the same sense, as they sometimes contain naphthol AS-D chloroacetate esterase positive Auer rods, which proves their neoplastic nature in such cases. Furthermore, very immature hemoblastoses can be identified as myeloid, if only few of the blasts reveal a positive naphthol AS-D chloroacetate esterase reaction. Finally it is mentioned that in rare cases, contrary to their normal behaviour, cosinophils may present with a positive naphthol AS-chloroacetate esterase reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01633225
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    The cytochemical demonstration of cyanide resistant peroxidase does not identify eosinophils selectively (1985)
    Springer
    Annals of hematology 51 (1985), S. 347-353 
    Details
    ISSN: 1432-0584
    Keywords: Cyanide resistant peroxidase ; Cytochemistry ; Eosinophils ; Neutrophils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Potassium cyanide (KCN) resistant peroxidase is generally accepted by hematologists as a selective stain for the eosinophilic cell line. However, it has been demonstrated biochemically that not only the peroxidases of neutrophils but also those of eosinophils can be inhibited by KCN. Therefore, bone marrow smears of hematologically normal patients were subjected to the peroxidase reaction in the presence of varying concentrations of KCN. It was found that with increasing concentrations of KCN not only neutrophils but also eosinophils were inhibited. Moreover, there were always neutrophilic promyelocytes that were still positive when a considerable number of the eosinophils was already inhibited. Therefore, it can be concluded from our results as well as from biochemical data that there is no concentration of KCN which demonstrates the total of the eosinophilic cell line selectively. The implications of these findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320045
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Myeloma with immunohistochemically different cell populations: Implication for the validity of immunohistochemical assessment of lymphoma cell monoclonality (1980)
    Springer
    Journal of molecular medicine 58 (1980), S. 409-414 
    Details
    ISSN: 1432-1440
    Keywords: Plasmozytom ; Immunhistochemie ; Monoclonalität ; Malignes Lymphom ; Plasmocytoma ; Immunohistochemistry ; Monoclonality ; Malignant lymphoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A case of myeloma showed histologically a monotonous pattern of relatively mature myeloma cells and an IgG-kappa paraproteinemia. In contrast to ordinary stains, the immunoperoxidase reactions for kappa and gamma gave inhomogeneous results. Light and heavy chains were formed by clusters of different subpopulations of myeloma cells. Serial sections showed that kappa-positive clusters were gamma-negative and vice versa. Thus, this morphologically homogeneous myeloma turned out to be immunohistochemically heterogeneous. We assume that two subpopulations have developed that produced separately kappa- and gamma-chains. Therefore, these chains might have been circulating separately and not as complete IgG-kappa molecules. Guided by our findings, the results of other investigators are discussed. Some considerations on the validity of immunohistochemical demonstration of tumor cell monoclonality are given implicating the diagnostic equivocation of immunohistochemistry for non-Hodgkin's lymphomas, especially for so-called “immunocytomas”.
    Notes: Zusammenfassung Ein Plasmozytomfall zeigte histologisch eine monotone, gleichmäßige Proliferation reifer Myelomzellen und eine IgG-Kappa-Paraproteinämie. Im Gegensatz zu histologischen Färbungen erbrachten die Immunperoxidase-Reaktionen für Kappa und Gamma ein inhomogenes Ergebnis. Leichte und schwere Ketten wurden von verschiedenen Subpopulationen der Plasmozytomzellen gebildet. Serienschnitte zeigten, daß Kappa-positive Areale Gamma-negativ waren und umgekehrt. So erwies sich dies morphologisch homogene Plasmozytom als immunhistochemisch heterogen. Wir nehmen an, daß sich zwei Subpopulationen entwickelt haben, die getrennt Kappa- und Gamma-Ketten bilden. Es ist möglich, daß diese Ketten auch getrennt zirkulierten und nicht als vollständige IgG-Kappa-Moleküle. Auf der Grundlage dieser Ergebnisse werden entsprechende Resultate anderer Untersucher diskutiert. Theoretische Überlegungen zum immunhistochemischen Nachweis einer Tumorzellmonoclonalität zeigen die fragwürdige Relevanz dieser Methodik für die Diagnostik der Nicht-Hodgkin-Lymphome auf, insbesondere für die sogenannten Immunozytome der Kiel-Klassifikation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01477506
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...