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  • 1970-1974  (4)
  • 1955-1959
  • 1971  (4)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 271 (1971), S. 193-203 
    ISSN: 1432-1912
    Schlagwort(e): Adrenal Medulla ; Chromogranin ; Catecholamines ; Synthesis ; Secretion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary 1. 3H-leucine and3H-tyrosine were added to the perfusion medium of isolated bovine adrenal glands in order to study the synthesis and secretion of radioactively labelled catecholamines and proteins. 2. Various times after the injection of the labelled amino acids the soluble proteins of the microsomal fraction were isolated and subjected to polyacrylamide gel electrophoresis in three different buffer systems. The distribution of protein-bound radioactivity within the gels was determined. Most of the label was found to be confined to proteins which behaved like the specific soluble proteins of chromaffin granules, i.e. the chromogranins. 3. Stimulation of the adrenal gland with carbachol induced a release of catechol-amines, protein, and protein-bound radioactivity. In the absence of Ca2+ the secretion of all these components was abolished. The highest specific radioactivity of the proteins secreted upon stimulation was reached 4 h after the injection of the labelled precursors. The labelled proteins, secreted upon stimulation, could be identified as chromogranins. 4. Carbachol induced the release of highly labelled catecholamines after the injection of3H-tyrosine. The highest specific radioactivity of these catecholamines was already observed at the first stimulation with carbachol i.e. 30 min after3H-tyrosine. 5. These results demonstrate that in isolated bovine adrenal glands radioactively labelled chromogranins and catecholamines can be synthesised and can be secreted upon stimulation with carbachol.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 270 (1971), S. 87-89 
    ISSN: 1432-1912
    Schlagwort(e): Adrenal Medulla ; Chromaffin Granules ; Sympathetic Nerve ; Membrane Protein ; Exocytosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A rabbit antiserum was obtained against a protein isolated from membranes of bovine chromaffin granules. Immunohistochemical evidence indicates that this protein is present in the adrenal medullae of several species and in bovine sympathetic nerve.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1432-2072
    Schlagwort(e): Vasopressin ; Epinephrine ; Norepinephrine ; Heart Rate ; Systolic Blood Pressure ; Diastolic Blood Pressure ; Urine Volume ; Performance Test ; Cold Application ; Pain Production
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The urinary excretion of vasopressin, epinephrine and norepinephrine was studied in normal subjects before and during three stress situations, viz. a performance test (consisting of mental arithmetic, the Stroop colour-word test, delayed auditory feedback and hand steadiness), the application of cold and the production of ischaemic muscle pain. The heart frequency, the systolic and diastolic blood pressure and the urine volume were measured as well. During the performance test the vasopressin, epinephrine and norepinephrine excretion increased significantly. During the application of cold only norepinephrine excretion increased significantly. During the production of muscle pain the vasopressin and epinephrine excretion increased significantly, the norepinephrine excretion rose, but not significantly. The systolic and diastolic blood pressure showed a significant increase during these three stress situations. The significant increase in heart rate was less pronounced during the cold and pain stress than during the performance stress. The urine volume was not significantly changed during the stress situations. These results were discussed in relation to the emotional arousal level during the performance test on one side and to the unpleasant but familiar conditions during the application of cold and the production of pain on the other side.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    The European physical journal 243 (1971), S. 166-170 
    ISSN: 1434-601X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Theg-factor of the 181 keV-level of99Tc has been redetermined by the spin rotation method. Measurements with polycrystalline sources of Tc in Fe, Co, and Ni yielded values of the hyperfine fields at the Tc nucleus. $$\begin{gathered} g = + 1.310(25) \hfill \\ H_{hf} (Tc{\mathbf{ }}in{\mathbf{ }}Fe) = ( - )290(15)kOe \hfill \\ H_{hf} (Tc{\mathbf{ }}in{\mathbf{ }}Co) = ( - )170(5)kOe \hfill \\ H_{hf} (Tc{\mathbf{ }}in{\mathbf{ }}Ni) = - 47.8(1.5)kOe. \hfill \\ \end{gathered} $$
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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