Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
23 (1974), S. 0
ISSN:
1471-4159
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Abstract— 〈list xml:id="l1" style="custom"〉1Chromatin protein fractions were separated from the nuclei from brain, liver and kidney of the guinea pig. The fractions were studied by electrophoretic methods and amino acid analysis.2Brain nuclear fractions were washed with 0.15m-NaCl and nuclear acidic proteins then removed by 0.35 m-NaCl. These 0.35 m-NaCl-extracted proteins were considered to be similar to the nuclear soluble acidic proteins.3Nonhistone-1, histone and nonhistone-2 fractions were obtained from 2.0 m-NaCl-soluble chromatin fractions by lowering the salt concentration and successive extraction with acid and alkali. The nonhistone-3 fraction was also extracted from the nuclear residue by alkaline solution.4The contents and characteristics of the nonhistone fractions of the brain, especially the nonhistone-1 fraction, differed among the three tissues. The histone fractions showed no obvious difference among the three tissues. The nonhistone-1 fraction of the brain, which comprised a low percentage of total nuclear protein, contained relatively high amounts of acidic proteins.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1471-4159.1974.tb06916.x
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