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  • 1
    Electronic Resource
    Electronic Resource
    IONIC, pH, AND TEMPERATURE EFFECTS ON THE BINDING ABILITY OF MYOSIN (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The peak force required to separate pieces of meat at a binding junction was measured with the Instron Universal Testing Machine. These measurements were used to study the effects of salt, phosphate, pH, cooking temperature and. protein concentration on the ability of crude myosin to bind meat pieces. Results showed salt and phosphate to linearly increase the ability of myosin to bind meat pieces primarily by solubilizing the protein. Temperature and the level of protein had increasing effects on binding ability in the range 45-80°C and 0-8%, respectively. Scanning electron micrographs of the ultrastructure of gels formed by myosin showed a three dimensional network of overlapping fibers in the presence of salt and phosphate and a sponge-like network in their absence. Since the ultrastructure of the gel formed by myosin heavy chains was comparable to that of myosin, it was concluded that the heavy chain core of the myosin molecule plays an important role in the heat-induced gelation of myosin. The relationship between the formation of a three dimensional network of fibers by the heavy chains of myosin and the mechanism of binding meat pieces was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1979.tb09116.x
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  • 2
    Electronic Resource
    Electronic Resource
    MlCROSTRUCTURE OF ISOLATED SOY PROTEIN IN COMBINATION HAM (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Combination hams were prepared by injecting excised porcine muscles with a brine containing solubilized isolated soy protein. The microstructure of the muscle in the combination ham was compared to that of a water added ham to determine the location of the isolated soy protein. The effect of massaging on the location of the isolated soy protein was also studied. The results showed that the isolated soy protein occupied primarily perimysial spaces and the massaging acts to incorporate these proteins into the endomysial spaces and mix them with extracted myofibrillar proteins. The isolated soy protein appeared to enhance myofibrillar protein extraction by binding water, thus increasing the effective concentration of salt and phosphate. Scanning electron microscopy showed that the gel formed by the isolated soy protein possessed a denser structure than the gel formed by a crude myosin preparation. The gel formed by a mixture of myosin and isolated soy protein possessed a structure more comparable to the structure of the crude myosin gel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1979.tb06417.x
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  • 3
    Electronic Resource
    Electronic Resource
    GEL STRUCTURE OF NONMEAT PROTEINS AS RELATED TO THEIR ABILITY TO BIND MEAT PIECES (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Investigations on the binding abilities of some nonmeat proteins showed that those studied were inferior to the binding ability previously reported for myosin. Their binding abilities in the presence of 8% salt and 2% sodium tripolyphosphate were ranked from highest to lowest as wheat gluten, egg white, corn gluten, calcium reduced dried skim milk, bovine blood plasma, isolated soy protein and sodium caseinate. Structures of gels formed by these proteins and mixtures of them with crude myosin showed that a three dimensional network of protein fibers is not indicative of good binding ability. The types of molecular interactions stabilizing their gel structures are thought to be more important.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1979.tb06418.x
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  • 4
    Electronic Resource
    Electronic Resource
    SENSORY, TEXTURAL AND YIELD PROPERTIES OF A COMBINATION HAM EXTENDED WITH ISOLATED SOY PROTEIN (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Combination hams were prepared by injecting deboned ham muscles with brines containing solubilized isolated soy protein. The muscles were injected to levels of 30%, 45% or 60%, and then subjected to either 0 hr or 18 hr of intermittent massaging. The effects of massaging, isolated soy protein, and the level of injection on binding strengths, cooking yields and taste-panel scores were examined. Massaging and isolated soy protein improved both binding strength and cooking yield. increasing levels of injection decreased binding strength and cooking yield. Massaging improved uniformity, textural appeal, and overall acceptability, but decreased tenderness and did not affect juiciness and flavor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1979.tb03443.x
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  • 5
    Electronic Resource
    Electronic Resource
    CRUDE MYOSIN FRACTIONS AS MEAT BINDERS (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 44 (1979), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A model system designed for the Instron Universal Testing Machine to record the peak force required to separate pieces of meat at a binding junction was used to measure the ability of various muscle protein fractions to bond meat pieces. The binding abilities of crude myosin preparations were found to be significantly greater than the binding ability of either a muscle homogenate free of fat and sarcoplasmic proteins, a total muscle homogenate or a nonprotein control consisting of salt, phosphate and water. Crude myosin fractions extracted from pre- and post-rigor bovine muscle over short and long extraction times with three extracting solutions, Guba-Straub, Hasselbach-Schneider and Weber-Edsall, were measured for binding ability, yield and mole ratio of myosin to actin by scanning SDS-PAGE gels. Results showed that the Hasselbach-Schneider myosin was superior to the Guba-Straub and Weber-Edsall myosins in both binding ability and yield. Comparisons of mole ratios with binding abilities showed that when the same extracting conditions were present the higher proportion of myosin resulted in a higher binding ability. However, when different extracting conditions were present, the higher proportion of myosin did not always give a higher binding ability. These results imply that ionic interactions are inculpated in the binding phenomenon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1979.tb03463.x
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  • 6
    Electronic Resource
    Electronic Resource
    Herzglykoside und Metaboliten — Probleme der Wiederfindung in Gewebsextrakten (1979)
    Springer
    International journal of legal medicine 83 (1979), S. 201-207 
    Details
    ISSN: 1437-1596
    Keywords: Digoxin ; Digoxin metabolites ; Radioimmunoassay, Digoxin and metabolites ; Digoxin ; Digoxinmetaboliten ; Radioimmunoassay, Digoxin und Metaboliten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Durch Wiederfindungsmessungen mit Hilfe von i.p. injiziertem3H-Digoxin und125-markiertem Digoxin konnte gezeigt werden, daß Standardkonzentrationen von Digoxin, die arzneistoffreiem Gewebe zugesetzt werden, zu falsch negativen Bestimmungen führen können. Der Vergleich der Radioaktivität aus verschiedenen Geweben vor und nach Extraktion mit Amberlite XAD-2 ergab, daß nur um 50% der gesamten Glykosidmenge extrahiert wird: BD9-(Berlin)-Ratten wurden 15mg/kg Körpergewicht β-Methyldigoxin i.p. injiziert. Aus Extrakten mit Amberlite XAD-2 von Blut, Niere, Herz und Leber konnten außer β-Methyldigoxin dessen Metaboliten Digoxin und Digoxinbidigitoxose ab 60 ng/Fleck in prozentualen Anteilen als Fluoreszenzflecke sichtbar identifiziert werden, durch die Anwendung von HPTLC- Platten mit Fluoreszenzzusatz. Radioimmunologisch bestimmte Konzentrationen fanden sich in guter Übereinstimmung mit dünnschichtchromatographischen Ergebnissen.
    Notes: Summary The recovery measurements in rat tissues performed via i.p. injected radioactive digoxin derivates (3H-digoxin,125-digoxin derivative) showed that approximately 50% of the total glycoside content will be extracted. Thus, an addition of digoxin standards to drug-free tissues may lead to false negative determinations. By comparison of the radioactivity before and after extraction the following results were obtained: After i.p. application of 15 mg/kg of β-methyldigoxin to BD 9 (Berlin)-rats the resulting tissue concentrations were extracted by Amberlite XAD-2. β- Methyldigoxin and its metabolites digoxin and digoxinbisdigitoxide could be separated and distinguished from artifacts by fluorescence detection on HPTLC-plates with a detection limit of 60 ng/spot. Concentrations determined by radioimmunoassay are in satisfactory agreement with HPTLC results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02333322
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  • 7
    Electronic Resource
    Electronic Resource
    Einfluß verschiedener Zytostatika auf die Proteinsynthese in leukämischen Plasmazellen (1979)
    Springer
    Annals of hematology 38 (1979), S. 155-159 
    Details
    ISSN: 1432-0584
    Keywords: Plasma cell leukemia ; Cell free protein synthesis ; Immunoglobulin synthesis ; Effect of BCNU
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary During the clinical course of a secondary plasma cell leukemia the cellular protein synthesis and immunoglobulin synthesis were measured in a cell-free system prepared from peripheral plasma cells. After treatment with cyclophosphamide and a combination of cyclophosphamide with melphalan and vincristine no change in the activity of total protein synthesis was found. In contrast, a decrease of total protein synthesis could be observed after treatment with BCNU combined with cyclophosphamide and Ara-C. The relative amount of 10–15% Ig-synthesis remained unchanged under the different chemotherapeutic regimes. These findings suggest a primary effect of BCNU on cellular protein synthesis in myeloma cells similar to that reported in HeLa cell cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007959
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  • 8
    Electronic Resource
    Electronic Resource
    The use of lymphocyte phosphoglucomutase as a genetic marker in bone marrow transplant recipients (1979)
    Springer
    Annals of hematology 38 (1979), S. 135-137 
    Details
    ISSN: 1432-0584
    Keywords: Knochenmarktransplantation ; Genetischer Marker ; Lymphozyten-Phosphoglukomutase ; Bone marrow transplantation ; Genetic marker ; Lymphocyte phosphoglucomutase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Lymphocyte phosphoglucomutase can be used as a genetic marker to document successful engraftment in bone marrow transplant recipients. Two patients who underwent marrow transplantation as a treatment for acute leukemia showed a change into donor-type isozyme pattern.
    Notes: Zusammenfassung Phosphoglukomutase aus Lymphozyten kann als genetischer Marker in der Knochenmarktransplantation verwendet werden. Nach Knochenmarktransplantation von zwei Patienten mit akuter Leukose konnte der Übergang in das Iso-Enzymmuster des Spenders beobachtet werden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007954
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  • 9
    Electronic Resource
    Electronic Resource
    The quadrupole interaction of100Rh in various intermetallic compounds of palladium (1979)
    Springer
    Hyperfine interactions 7 (1979), S. 247-252 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The nuclear quadrupole interaction of the 75 keV excited state of100Rh in the ordered intermetallic compounds PdHg, PdPb2 PdSb and PdTe was measured. Using an estimate for the nuclear quadrupole moment of the 75 keV state in100Rh and point ion lattice sums for the lattice electric field gradient (EFG) at the Rh site, the electronic contributionV zz el to the total EFG was derived.V zz el was found to be correlated with the average valence electron concentration in the compounds studied. In the case of PdPb2, the temperature dependence of the quadrupole interaction was also studied and found not to follow theT 3/2 rule which is observed in virtually all pure noncubic metals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01021507
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