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  • 1975-1979  (3)
  • 1979  (3)
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  • 1975-1979  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Anomeric preferences ofd-glucose uptake and utilization by cerebral cortex slices of rats (1979)
    Springer
    Neurochemical research 4 (1979), S. 505-516 
    Details
    ISSN: 1573-6903
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract On aerobic incubation of rat cerebral cortex slices with anomers ofd-glucose and with 2-deoxy-d-glucose (2DG) for 5 min, the disappearance of β-d-glucose from the incubation mixture was greater than that of α-d-glucose and both anomers had a greater rate of disappearance than that of 2DG. In addition, there were significantly greater consumption of oxygen and production of lactate with the β-anomer than with the α-anomer. In similar experiments with3H-labeledd-glucose anomers and [1-3H]-3-O-methyl-d-glucose (3MG), the accumulation of [1-3H]-β-d-glucose (up to 5 min) by rat cerebral cortex slices was greater than that of [1-3H]-α-d-glucose. Although initially lower than that of the anomers, the accumulation of [1-3H]-3MG increased at a greater rate and, by 5 min of incubation, was greater than that of both glucose anomers. This preferential accumulation was seen to disappear when the slices were preincubated with 2DG (hexokinase inhibitor) or when the temperature of incubation was reduced to 20°C. Under those conditions the data with the glucose anomers were similar to those obtained with 3MG. Our data then suggested that the greater accumulation of β-d-glucose than of α-d-glucose by the slices was probably not due to differences in transport through brain cell membranes but rather to the preferential metabolism of the β-d-glucose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00964644
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The localization of mutarotase in rat kidney (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1094-1097 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949964
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Different effects of D-glucose anomers for respiration of bacterial germinated spores (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 470-472 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Effects of α- or β-D-glucose on the respiration of germinated spores (only germinated spores not including swollen spores and elongated spores) ofBacillus subtilis andB. megaterium were studied. In our conditions, net amount of oxygen consumed by 1010 germinated spores ofB. subtilis per min after addition of α- or β-D-glucose was 1.6 μg or 6.6 μg (β/α=4.13), while that byB. megaterium was 4.5 μg or 6.8 μg (β/α=1.51), respectively. However, the net amounts of oxygen consumed by 1010 vegetative cells per min after addition of α- or β-D-glucose were identical, forB. subtilis in both cases 443.0 μg and forB. megaterium in both cases 604.4 μg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01922712
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    Paper (German National Licenses)
    Fulltext
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