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  • 1
    Electronic Resource
    Electronic Resource
    Specific determination of arylsulfatase A activity (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 33-35 
    Details
    ISSN: 1420-9071
    Keywords: Arylsulfatase A ; natural substrate ; ascorbate-2-sulfate ; HPLC-amperometric detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01975881
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Clinical pathology of primitive gliomas in the cerebrum (1986)
    Springer
    Acta neurochirurgica 81 (1986), S. 94-99 
    Details
    ISSN: 0942-0940
    Keywords: Primitive glioma ; ependymoblastoma ; undifferentiated glioma ; unclassified glioma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To clarify the biological features of primitive gliomas in the cerebrum and clearly distinguish them from malignant or anaplastic gliomas and glioblastomas, we studied eight cases clinically and pathologically. Our evaluations included immunohistochemical and electron microscopic observations. We divided the patients into two groups, children and young adults. Most tumours appeared as ring-like, enhanced masses on computed tomography and avascular or ring-like, vascular masses on angiography. Macroscopically, the tumours were well demarcated and contained cysts. Ocassionally we found tumour dissemination. Microscopically, the tumours were composed of small, round cells without remarkable structural features. Ependymal, astroglial, and oligodendroglial differentiation was evident, in varying proportions; tumours in which the differentiated areas constituted more than half of the mass were classified as poorly differentiated gliomas. By these criteria, this series comprised four undifferentiated and four poorly differentiated gliomas. Cell anaplasia and polymorphism were rare in both undifferentiated and differentiated areas of the tumours. Immunohistochemical and electron microscopic examinations also revealed glial differentiation. These primitive gliomas appear to be biologically similar, but not identical, to cerebellar medulloblastomas. In this series, five patients died because of recurrence or dissemination. Whole brain and spinal irradiation should be considered after total or subtotal surgical removal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01401228
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    High level radioactive nuclides in Japan in May (1986)
    [s.l.] : Nature Publishing Group
    Nature 321 (1986), S. 819-820 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR-The accidental release of radioactive materials from the Chernobyl nuclear power plant on 26 April 1986 is known to have caused significant pollution in Western Europe at distances as great as 2,000 km. We have been measuring natural and artificial radionuclides in the environment of Japan for ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/321819a0
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  • 4
    Electronic Resource
    Electronic Resource
    Mössbauer sprectroscopic characterization of iron chlorophyllins (1986)
    Springer
    Hyperfine interactions 29 (1986), S. 1403-1406 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The Mössbauer spectra of bis-adducts of pyridine (py) and its derivatives (4-Xpy, where X is CN, CHO, COOCH3, COCH3 and CH3) have revealed that the axial coordination of py and 4-Xpy to iron (III) chlorophyllin gives rise to spontaneous reduction of the central iron (III) ion. A linear correlation between isomer shifts and quadrupole splittings is explained on the basis of the predominant σ-donation from the axial ligand to the central iron ion. The linear dependence of the isomer shifts on the Hammett substituent constants has demonstrated that the larger the isomer shift, the stronger the electron-attracting power of the substituent at the 4-position. The decrease in the quadrupole splitting is ascribed to the relative decrease in the electron density accumulated in the equatorial direction through the σ-donation. The general tendency in the spontaneous reduction of iron (III) chlorophyllin is interpreted in terms of the σ-donor and π-acceptor power of the axial ligands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02399496
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of organic solvents on the activity of glucoamylase (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 1172-1177 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hydrolyses of maltose, maltotriose, and soluble starch catalyzed by glucoamylase (Asp. Niger) were carried out in the aqueous solutions of methanol, ethanol, ethylene glycol, and 1,4-dioxane at 40°C and at the optimum pH in the respective solutions. By the kinetic analysis based on the subsite model, it was shown that the intrinsic rate constant, Kint, was electrostatically affected by the dielectric constant of the hydroorganic solutions. The affinity of the third subsite, A3, which affects the apparent rate constant, K0, was correlated with the ×Gtr's of maltose and amino acid side chains.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260280807
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    Paper (German National Licenses)
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