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  • 1985-1989  (4)
  • 1950-1954
  • 1986  (4)
Materialart
Erscheinungszeitraum
  • 1985-1989  (4)
  • 1950-1954
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Bulletin of environmental contamination and toxicology 36 (1986), S. 851-857 
    ISSN: 1432-0800
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik , Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    The journal of membrane biology 90 (1986), S. 89-96 
    ISSN: 1432-1424
    Schlagwort(e): frog skin ; membrane potential ; voltage clamp ; K+ depolarization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary In studies of apical membrane current-voltage relationships, in order to avoid laborious intracellular microelectrode techniques, tight epithelia are commonly exposed to high serosal K concentrations. This approach depends on the assumptions that high serosal K reduces the basolateral membrane resistance and potential to insignificantly low levels, so that transepithelial values can be attributed to the apical membrane. We have here examined the validity of these assumptions in frog skins (Rana pipiens pipiens). The skins were equilibrated in NaCl Ringer's solutions, with transepithelial voltageV t clamped (except for brief perturbations ΔV t) at zero. The skins were impaled from the outer surface with 1.5m KCl-filled microelectrodes (R el〉30 MΩ). The transepithelial (short-circuit) currentl i and conductanceg t=−ΔI t/ΔV t, the outer membrane voltageV o (apical reference) and voltage-divider ratio (F o=ΔV o/ΔV t), and the microelectrode resistanceR el were recorded continuously. Intermittent brief apical exposure to 20 μm amiloride permitted estimation of cellular (c) and paracellular (p) currents and conductances. The basolateral (inner) membrane conductance was estimated by two independent means: either from values ofg i andF o before and after amiloride or as the ratio of changes (−ΔI c/ΔV i) induced by amiloride. On serosal substitution of Na by K, within about 10 min,I c declined andg t increased markedly, mainly as a consequence of increase ing p. The basolateral membrane voltage (V i(=−V o) was depolarized from 75±4 to 2±1 mV [mean±sem (n=6)], and was partially repolarized following amiloride to 5±2 mV. The basolateral conductance increased in high serosal K, as estimated by both methods. Essentially complete depolarization of the basolateral membrane and increase in its conductance in response to high [K] were obtained also when the main serosal anion was SO4 or NO3 instead of Cl. On clampingV t over the range 0 to +125 mV in K2SO4-depolarized skins, the quasi-steady-stateV o V t relationship was linear, with a mean slope of 0.88±0.03. The above results demonstrate that, in a variety of conditions, exposure to high serosal K results in essentially complete depolarization of the basolateral membrane and a large increase in its conductance.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    The journal of membrane biology 92 (1986), S. 37-46 
    ISSN: 1432-1424
    Schlagwort(e): frog skin ; cell Na activity ; membrane potential ; Na pump flux ; Na microelectrodes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary Cell Na activity,a Na c , was measured in the short-circuited frog skin by simulaneous cell punctures from the apical surface with open-tip and Na-selective microelectrodes. Skins were bathed on the serosal surface with NaCl Ringer and, to reduce paracellular conductance, with NaNO3 Ringer on the apical surface. Under control conditionsa Na c averaged 8±2mm (n=9,sd). Apical addition of amiloride (20 μm) or Na replacement reduceda Na c to 3mm in 6–15 min. Sequential decreases in apical [Na] induced parallel reductions ina Na c and cell current,I c . On restoring Na after several minutes of exposure to apical Na-free solutionI c rose rapidly $$(\tilde〈 30\sec )$$ to a stable value whilea Na c increased exponentially, with a time constant of 1.8±0.7 min (n=8). Analysis of the time course ofa Na c indicates that the pump Na flux is linearly related toa Na c in the range 2–12mm. These results indicate thata Na c plays an important role in relating apical Na entry to basolateral active Na flux.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Water, air & soil pollution 28 (1986), S. 1-26 
    ISSN: 1573-2932
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Energietechnik
    Notizen: Abstract Rye-grass, Lolium multiflorum, cultures on perlite, an artificial soil, were carried out with a basic nutrient solution (modified in each experiment), adding double distilled water when necessary to restore evaporation losses. Cultures were collected at different times, separated into roots and tops and controlled by moisture, dry weights and metallic element contents measurements. Plant chemical analyses were performed after HF-HN03-HClO4 digestion by AAS (Mg, Ca, Fe, Mn, Cu, Zn, Co, Ni, Cd, As, Pb) and flame emission (Na and K). Cultures with changes in basic nutrient solution component concentrations, with additions of potentially toxic elements (Co, Ni, Cd, As, Pb, Cu, Zn, Na) and with different periods of culture (7 to 49 days after 6 days for germination) were carried out. Measurements of metallic elements dissolution and diffusion within a perlite layer were also made. For discussion of results ionic impulsions are introduced as I = c 1/n , where c is the considered metallic element concentration and n its oxidation number. Root or top uptakes of a metallic element follow linear relationships with their ionic impulsions in nutrient solution or root, respectively. Roots and tops seem to keep constant the total ionic impulsions, sums of individual metallic elements ionic impulsions. Toxic effects were evident for total ionic impulsions greater than average values. Results are further discussed through NUTRIENT SOLUTION ⇔ PERLITE ⇔ ROOT ⇔ TOP equilibria that suggest a single assimilation mechanism for roots, and two different ones for ROOT ⇒ TOP step: (i) an osmotic permeation with root accumulation of some elements (Cu, Zn, Fe, Co, Ni, Cd, As, Pb) and (ii) a DONNAN permeation leading to top accumulation of other metals (Mg, K, Ca, Na, Mn).
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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