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  • 1
    Digitale Medien
    Digitale Medien
    Effect of salt stress on interaction between lentil (Lens culinaris) genotypes and Rhizobium spp. strains: symbiotic N2 fixation in normal and sodic soils (1999)
    Springer
    Biology and fertility of soils 29 (1999), S. 187-195 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Key words Rhizobium spp. ; Symbiotic N2 fixation ; Nitrogenase activity ; Glutamine synthetase ; Sodic soils
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract  Following screening, selection, characterization and examination of their symbiotic N2 fixation, only two Rhizobium strains (ND-16 and TAL-1860) and four lentil genotypes (DLG-103, LC-50, LC-53 and Sehore 74-3) were found to be suited to sodic soils. Interactions between salt-tolerant lentil genotypes and Rhizobium strains were found to be significant, and resulted in greater nodulation, N2 fixation (nitrogenase activity), total nitrogen, plant height, root length and grain yield in sodic soils under field conditions compared to uninoculated controls. Significantly more nodulation, nitrogenase activity, glutamine synthetase (GS) and NADH-dependent glutamate synthase (NADH-GOGAT) activities were found in normal soil as compared to the soil supplemented with 4% and 8% NaCl. Salt stress inhibited nitrogenase, GS and NADH-GOGAT activities. However, nitrogenase activity in nodules was more sensitive to salt stress than GS and NADH-GOGAT activities (NH4 + assimilation). The relevance of these findings for salt-tolerant symbionts is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s003740050543
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  • 2
    Digitale Medien
    Digitale Medien
    Two types of ELF hiss observed at Varanasi, India (1999)
    Springer
    Annales geophysicae 17 (1999), S. 1260-1267 
    Details
    ISSN: 0992-7689
    Schlagwort(e): Ionosphere (equatorial ionosphere; ionosphere-magnetosphere interactions; wave-particle interactions)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie , Physik
    Notizen: Abstract The morphology of ELF hiss events observed at low-latitude ground station Varanasi (L = 1.07, geomagnetic latitude 14°55′N) are reported, which consist of two types: (1) events which propagated in ducted mode along the geomagnetic field line corresponding to observing station Varanasi and (2) events which propagated in ducted mode along higher L-values (L = 4–6), after reaching the lower edge of ionosphere excite the Earth-ionosphere wave guide and propagate towards equator to be received at Varanasi. To understand the generation mechanism of ELF hiss, incoherent Cerenkov radiated power from the low latitude and middle latitude plasmasphere are evaluated. Considering this estimated power as an input for wave amplification through wave-particle interaction, the growth rate and amplification factor is evaluated which is too small to explain the observed wave intensity. It is suggested that some non-linear mechanism is responsible for the generation of ELF hiss.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s00585-999-1260-5
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  • 3
    Digitale Medien
    Digitale Medien
    Influence of bcl-2 on antibody productivity in high cell density perfusion cultures of hybridoma (1999)
    Springer
    Cytotechnology 30 (1999), S. 95-106 
    Details
    ISSN: 1573-0778
    Schlagwort(e): apoptosis ; Bcl-2 ; fixed-bed ; hollow fibre ; hybridoma ; perfusion ; protein-free medium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Apoptosis is an active, genetically determined death mechanism which can be induced by a wide range of physiological factors and by mild stress. It is the predominant form of cell death during the production of antibodies from murine hybridoma cell lines. A number of studies have now demonstrated that the suppression of this death pathway, by means of over-expression of survival genes such as bcl-2, results in improved cellular robustness and antibody productivity during batch culture. In the present study, the influence of bcl-2 expression on hybridoma productivity in two high density perfusion bioreactor systems was investigated. In the first system, a fixed-bed reactor, the DNA content in the spent medium was 25% higher in the control (TB/C3-pEF) culture than that found in the bcl-2 transfected (TB/C3-bcl2) cultures at all perfusion rates. This is indicative of a higher level of cell death in the control cell line. The average antibody concentration for the TB/C3-pEF cell line was 14.9 mg L-1 at perfusion rates of 2.6 and 5.2 d-1. However, for the TB/C3-bcl2 cell line it was 33 mg L-1 at dilution rates of 2 and 4 d-1. A substantial increase in antibody concentration was also found in the Integra Tecnomouse hollow fibre reactor. The antibody titre in the TB/C3-bcl2 cassette was nearly 100% higher than that in the TB/C3-pEF cassette during the cultivation period which lasted 6 weeks. Clearly, these results demonstrate the positive impact of bcl-2 over-expression on production of antibody in hybridoma perfusion cultures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008055702079
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  • 4
    Digitale Medien
    Digitale Medien
    Tests of Predictive Viscosity Models for Pure Liquids (1999)
    Springer
    International journal of thermophysics 20 (1999), S. 815-823 
    Details
    ISSN: 1572-9567
    Schlagwort(e): alkanes ; alkanols ; alkenes ; aromatics ; corresponding states ; cycloalkanes ; esters ; liquids ; viscosity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract It is of considerable importance to be able to predict accurately the viscosity of liquids over a wide range of conditions. In the present work, the ability of the three-parameter generalized corresponding states principle (GCSP) for the prediction of the viscosity of pure liquids is demonstrated. The viscosity of six different classes of pure liquids, viz., alkanes (19 compounds; 207 data points), cycloalkanes (6 compounds; 74 data points), alkenes (9 compounds; 146 data points), aromatics (4 compounds; 123 data points), alkanols (8 compounds; 89 data points), and esters (4 compounds; 28 data points) have been predicted over a wide range of temperatures using the three-parameter (T c, P c, θ) GCSP. Five options for the third parameter (θ) were studied, viz., Pitzer's acentric factor ω, molar mass M, characteristic viscosity η*, critical compressibility factor Z c, and modified acentric factor Ω, in addition to groups ωZ c and ΩZ c being treated as composite third parameters. Pressure effects were neglected. Good agreement between experimental and predicted values of viscosity was obtained, especially with either ω or η* being used as the third parameter. Furthermore, the viscosities of alkanes predicted by the TRAPP method and an empirical, generalized one-parameter model for liquid hydrocarbons provide comparisons with the more accurate GCSP method. The GCSP provides a simple and yet a powerful technique for the correlation and prediction of viscosities of a variety of pure liquids over a wide range of temperatures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1022674917267
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