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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 23 (2000), S. 0 
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The fracture surfaces of specimens of a heat-treated hard steel, namely Cr–Mo steel SCM435, which failed in the regime of N = 105 to 5 × 108 cycles, were investigated by optical microscopy and scanning electron microscopy (SEM). Specimens having a longer fatigue life had a particular morphology beside the inclusion at the fracture origin. The particular morphology looked optically dark when observed by an optical microscope and it was named the optically dark area (ODA). The ODA looks a rough area when observed by SEM and atomic force microscope (AFM). The relative size of the ODA to the size of the inclusion at the fracture origin increases with increase in fatigue life. Thus, the ODA is considered to have a crucial role in the mechanism of superlong fatigue failure. It has been assumed that the ODA is made by the cyclic fatigue stress and the synergetic effect of the hydrogen which is trapped by the inclusion at the fracture origin. To verify this hypothesis, in addition to conventionally heat-treated specimens (specimen QT, i.e. quenched and tempered), specimens annealed at 300 °C in a vacuum (specimen VA) and the specimens quenched in a vacuum (specimen VQ) were prepared to remove the hydrogen trapped by inclusions. The specimens VA and VQ, had a much smaller ODA than the specimen QT. Some other evidence of the influence of hydrogen on superlong fatigue failure are also presented. Thus, it is concluded that the hydrogen trapped by inclusions is a crucial factor which causes the superlong fatigue failure of high strength steels.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 23 (2000), S. 0 
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: High cycle fatigue fracture surfaces of specimens in which failure was initiated at a subsurface inclusion were investigated by atomic force microscopy and by scanning electron microscopy. The surface roughness Ra increased with radial distance from the fracture origin (inclusion) under constant amplitude tension–compression fatigue, and the approximate relationship: Ra ≅ CΔK 2I holds. At the border of a fish-eye there is a stretched zone. Dimple patterns and intergranular fracture morphologies are present outside the border of the fish-eye. The height of the stretch zone is approximately a constant value around the periphery of the fish-eye. If we assume that a fatigue crack grows cycle-by-cycle from the edge of the optically dark area (ODA) outside the inclusion at the fracture origin to the border of the fish-eye, we can correlate the crack growth rate da/dN, stress intensity factor range ΔKI and Ra for SCM435 steel by the equation 〈inlineGraphic alt="inline image" href="urn:x-wiley:8756758X:FFE343:FFE_343_m81" location="equation/FFE_343_m81.gif"/〉 and by da/dN proportional to the parameter Ra .Integrating the crack growth rate equation, the crack propagation period Np2 consumed from the edge of the ODA to the border of the fish-eye can be estimated for the specimens which failed at Nf 〉 107. Values of Np2 were estimated to be ∼1.0 × 106 for the specimens which failed at Nf ≅ 5 × 108. It follows that the fatigue life in the regime of Nf 〉107 is mostly spent in crack initiation and discrete crack growth inside the ODA.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 327-328 (Jan. 2000), p. 441-444 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Bowen’s disease (BD) is a squamous cell carcinoma in situ that rarely invades into the underlying dermis. However, little is known about its immunohistology. Objectives To evaluate the relationship between the cytological properties of the tumour cells in BD and the host immune response. Methods We examined the expression of p53, proliferating cell nuclear antigen (PCNA) and Ki67 antigen, and the number of mitotic cells, together with the number of intratumoral and dermal infiltrating CD1a+, CD3+, CD4+, CD8+, CD68+ and cutaneous lymphocyte-associated antigen (CLA)+ cells in 18 cases of genital BD. Results When compared with normal genital skin (n = 10), there was a significantly higher number of mitotic cells as well as higher expression of p53+, PCNA+ and Ki67+ cells in BD. There was significant mutual correlation between CD3+, CD4+ and CD68+ cells in the tumoral epidermis. The number of CD1a+ Langerhans cells significantly decreased in BD epidermis; however, dermal CD1a+ cells were increased. Interestingly, numbers of dermal CD1a+ cells significantly correlated with those of intratumoral CD3+, CD4+ and CD68+ cells. In situ hybridization for human papillomavirus (HPV) demonstrated that HPV-infected BD had significantly less infiltration of intratumoral CD3+ cells and CLA+ cells. Conclusions The present data suggest that dermal CD1a+ cells may participate in the immune surveillance and that HPV infection may interfere with the intratumoral infiltration of CLA+ cells in BD.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 143 (2000), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We describe a 70-year-old man with cutaneous granulocytic sarcoma who presented with numerous cutaneous nodules but without any leukaemic involvement of the peripheral blood. The tumour cells were positive for lysozyme, peroxidase, CD11a, CD11c, CD33 and HLA-DR, and weakly positive for CD4 and CD14, suggesting granulocytic differentiation. The bone marrow at admission showed dysplasia of the erythrocytic and granulocytic lineage and complex chromosomal abnormalities in association with an increase in monocytes. The patient was diagnosed as having granulocytic sarcoma of monocytic lineage with concomitant myelodysplastic syndrome. In this case, tumour cells also expressed the neural cell adhesion molecule (CD56), which has been suggested as a possible risk factor for developing granulocytic sarcoma in acute myelogenous leukaemia.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 33 (2000), S. 1241-1245 
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: A synchrotron radiation X-ray powder diffractometer for samples of very small amount has been developed to collect high-quality diffraction patterns under extreme conditions, i.e. at low temperature and/or high pressure. A new cylindrical imaging plate (CIP) is used as a detector, in addition to a conventional flat-type imaging plate (FIP). By using the CIP system, the diffraction data in a diffraction angle range −44 ≤ 2θ ≤ 122° are collected with a dynamic range of about 106. The alignment of the diffractometer, measurement and analysis are automatically operated by a workstation. A performance test shows that the CIP system has spatial resolution of about 0.07° with a dynamic range of 106. The diffraction pattern of a standard sample of Si measured by the CIP system has high quality; the refinement of the structure reaches Rw = 3.68% even in the case of a small amount of sample (about 2 µg) and a short exposure time (60 s). Examples of experiments at low temperatures under ambient and high pressures are also presented.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Clinical and experimental nephrology 4 (2000), S. 293-299 
    ISSN: 1437-7799
    Keywords: Key words Calcium receptor ; HEK293 ; NPS R-568 ; Calcimimetics ; Intracellular Ca2+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. The Ca2+ receptor (CaR) plays a key role in maintaining Ca2+ homeostasis by its presence in the parathyroid gland and kidney. NPS R-568 (referred to as KRN568 in Japan) is a phenylalkylamine compound that activates the CaR. It has been difficult to study Ca2+-sensing mechanisms because of the lack of cell model systems that express reasonable numbers of CaR coupled to downstream effectors and physiological responses. This study was conducted to evaluate the effects of NPS R-568 on the CaR both in vitro and in vivo. Methods. Western blotting analysis of CaR was performed to confirm the existence of CaR in human embryonic kidney 293 (HEK293) cells expressing human CaR (HuCaR-HEK293). Intracellular Ca2+ concentration ([Ca2+]i) and inositol trisphosphate (IP3) content were measured in HuCaR-HEK293 after the addition of NPS R-568 and other agonists. Male Sprague-Dawley rats received NPS R-568 orally, and plasma Ca2+ levels and serum parathyroid hormone (PTH) levels were determined. Results. Western blotting analysis of the crude plasma membrane fraction prepared from HuCaR-HEK293 identified bands immunoreactive with a human CaR-specific antibody. NPS R-568 dose-dependently and stereoselectively increased [Ca2+]i in HuCaR-HEK293, whereas NPS R-568 had no effects in wild-type HEK293 cells. These effects of NPS R-568 were associated with an increase in cytoplasmic IP3 levels and were abolished in the absence of extracellular Ca2+. Single oral administration of NPS R-568 suppressed PTH secretion, followed by decreased plasma Ca2+ levels, in normal rats. Conclusions. These results suggest that NPS R-568 activates CaR and suppresses PTH secretion in vitro and in vivo.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1435-232X
    Keywords: Key words Microcell-mediated chromosome transfer ; Chromosome 10p ; Rodent–human hybrid ; Single transferable fragment ; Tumor suppressor gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The introduction of chromosome 10p into human glioblastoma or prostate cancer cells has been demonstrated to suppress their malignant phenotype, suggesting the presence of glioma or prostate tumor suppressor genes on 10p. As a resource for the fine mapping of these genes, a series of human-rodent hybrid cell lines containing single transferable fragments (STFs) of 10p were constructed. Normal chromosome 10 tagged with a neomycin-resistance gene on its short arm was fragmented by gamma-irradiation of 5–10 krad, transferred into mouse L cells or Chinese hamster ovary cells by microcell-mediated chromosome transfer (MMCT), and then selected against G418. Thirty-three independent rodent-human hybrids carrying various-sized STFs were obtained. Polymerase chain reaction (PCR)-based genotyping revealed that these STFs contained the whole, or portions, of a 43-cM region on 10p14-pter and could be defined by 19 sequence-tagged-site (STS) markers. Using this panel of hybrids as donors for further MMCT, genes on the refined fragments could be transferred into other cells. This hybrid panel would therefore be a useful resource for the fine mapping of the genes on 10p14-pter to segments of about 2.4 cM by functional complementation.
    Type of Medium: Electronic Resource
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