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  • 2000-2004  (2)
  • 2003  (1)
  • 2002  (1)
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  • 2000-2004  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Physiologia plantarum 117 (2003), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Non-grafted tomato plants (Lycopersicon esculentum L. cv. Tmknvf2) and grafted tomato plants (L. esculentum L. cv. Tmknvf2 × L. esculentum L. cv. RX-335) were grown for 30 days at three different temperatures (10°C, 25°C and 35°C). In the leaves of these plants, the enzymatic activities of SOD, GPX, CAT, APX, DHAR and GR were analysed, as were the concentrations of total H2O2, ascorbate and glutathione as well as foliar DW. Regardless of whether the plant was grafted or not, our results indicate that the thermal stress occurred mainly at 35°C, with the following effects: (1) high SOD activity; (2) H2O2 accumulation; (3) foliar-biomass reduction; (4) low GPX, CAT, APX, DHAR and GR activities; and (5) high concentrations of ascorbate and glutathione. In addition, our data show these effects to be much weaker in grafted than in non-grafted plants, directly reflected in greater biomass production. Therefore, the use of grafted plants under excessively high temperatures may offer an advantage over non-grafted plants in terms of resistance against thermal shock.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 212 (2002), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Our previous results have demonstrated that Phanerochaete flavido-alba decoloration, dephenolization and detoxification of olive oil mill wastewater (OMW) were associated with changes in the ligninolytic major exoenzymes accumulated in the cultures. This paper describes the effect of the two main OMW components (monomeric aromatic compounds and a major brownish polymeric pigment), on extracellular P. flavido-alba ligninolytic enzymes. Laccase was the sole ligninolytic enzyme detected in cultures containing monomeric aromatic compounds. Laccase and an acidic manganese-dependent peroxidase (MnPA, pI〈2.8) were accumulated in cultures with OMW or polymeric pigment. Also, modified manganese-dependent peroxidases were observed mainly in OMW-supplemented cultures. Laccase was more stable to the effect of OMW toxic components and was accumulated in monomeric aromatic-supplemented cultures, suggesting a more important role than manganese-dependent peroxidases in OMW detoxification. Alternatively, MnPA accumulated in cultures containing the polymeric pigment seems to be more essential than laccase for degradation of this recalcitrant macromolecule by P. flavido-alba.
    Type of Medium: Electronic Resource
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