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  • 2000-2004  (3)
  • 2003  (3)
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  • 2000-2004  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Removal of PSA from NCAM affects the survival of magnocellular vasopressin- and oxytocin-producing neurons in organotypic cultures of the paraventricular nucleus (2003)
    Oxford, UK : Blackwell Science, Ltd
    European journal of neuroscience 17 (2003), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The expression of the polysialic acid neural cell adhesion molecule (PSA-NCAM) in the hypothalamo-neurohypophyseal system has been correlated with morphofunctional plasticity. In this study, we investigated the role of PSA-NCAM in the survival of oxytocin (OT)- and vasopressin (VP)-producing magnocellular cells of this system. We used a recently developed organotypic slice culture model of the rat hypothalamic paraventricular nucleus (PVN) in which ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) are potent survival factors for magnocellular neurons. We demonstrate by means of confocal microscopy that cultured magnocellular VP and OT neurons express strong immunoreactivity for PSA-NCAM. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons in the presence of low concentrations of CNTF. Endo N treatment did not change cell survival in the presence of LIF. These results suggest that, in addition to its role in neuro-glial plasticity, PSA-NCAM might also influence the trophic factor responsiveness of hypothalamic VP and OT neurosecretory cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-9568.2003.02660.x
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for vesicular glutamate transporter synapses onto gonadotropin-releasing hormone and other neurons in the rat medial preoptic area (2003)
    Oxford, UK : Blackwell Science Ltd
    European journal of neuroscience 18 (2003), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The medial preoptic area is a key structure in the control of reproduction. Several data suggest that excitatory amino acids are involved in the regulation of this function and the major site of this action is the medial preoptic region. Data concerning the neuromorphology of the glutamatergic innervation of the medial preoptic area are fragmentary. The present investigations were focused on: (i) the morphology of the vesicular glutamate transporter 1 (VGluT1)- and vesicular glutamate transporter 2 (VGluT2)-immunoreactive nerve terminals, which are considered to be specific to presumed glutamatergic neuronal elements, in the medial preoptic area of rat; and (ii) the relationship between these glutamate transporter-positive endings and the gonadotropin-releasing hormone (GnRH) neurons in the region. Single- and double-label immunocytochemistry was used at the light and electron microscopic level. There was a weak to moderate density of VGluT1- and a moderate to intense density of VGluT2-immunoreactive elements in the medial preoptic area. Electron microscopy revealed that both VGluT1- and VGluT2-immunoreactive boutons made asymmetric type synaptic contacts with unlabelled neurons. VGluT2-labelled, but not VGluT1-labelled, axon terminals established asymmetric synaptic contacts on GnRH-immunostained neurons, mainly on their dendrites. The present findings are the first electron microscopic examinations on the glutamatergic innervation of the rat medial preoptic area. They provide direct neuromorphological evidence for the existence of direct glutamatergic innervation of GnRH and other neurons in the rat medial preoptic area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-9568.2003.03085.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Somaclonal breeding of reed canarygrass (Phalaris arundinacea L.) (2003)
    Oxford, UK : Blackwell Science Ltd
    Grass and forage science 58 (2003), S. 0 
    Details
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Tissue culture-induced variants (somaclones) of reed canarygrass (Phalaris arundinacea L.) were developed from callus cells of aseptic spikelet cultures. Molecular polymorphism of the populations of somaclones and spikelet-donor plants as natural vegetative clones were compared by the application of polymerase chain reaction (PCR)-based techniques of random amplified polymorphic DNA (RAPD)-, simple sequence repeat (SSR)-, and inter-simple sequence repeat (ISSR)-PCR to genomic DNA samples. The genetic diversity was higher in the somaclones compared with vegetative clones as measured by the maximum genetic distance (MaxGD) which increased from 0·401 (vegetative clones) to 0·498 (somaclones). Along with this change, the minimum genetic distance (MinGD) decreased from 0·20 (control plants) to 0·06 (somaclones) which demonstrated that genetic changes occurred not only in a positive (dissimilarity, MaxGD), but also in a negative (similarity, MinGD) direction. Structural carbohydrate analyses were also performed on the leaves to compare the somaclones with the donor vegetative clones. The mean neutral-detergent and acid-detergent fibre concentrations of the leaves of the somaclones were about 0·20 lower than the donor vegetative clones suggesting that increases in the digestibility could be obtained from the somaclonal material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2494.2003.00372.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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