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Identification of three genes expressed primarily during development in Physarum polycephalum (1999)

Bailey, J. ; Cook, L. J. ; Kilmer-Barber, R. ; [et al.]

Springer

Archives of microbiology 172 (1999), S. 364-376

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ISSN: 1432-072X

Keywords: Key words Slime moulds ; Physarum polycephalum ; Plasmodium development ; Differential gene expression ; Myosin ; Calcium-binding protein

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract During the life cycle of Physarum polycephalum, uninucleate amoebae develop into multinucleate syncytial plasmodia. These two cell types differ greatly in cellular organisation, behaviour and gene expression. Classical genetic analysis has identified the mating-type gene, matA, as the key gene controlling the initiation of plasmodium development, but nothing is known about the molecular events controlled by matA. In order to identify genes involved in regulating plasmodium formation, we constructed a subtracted cDNA library from cells undergoing development. Three genes that have their highest levels of expression during plasmodium development were identified: redA, redB (regulated in development) and mynD (myosin). Both redA and redB are single-copy genes and are not members of gene families. Although redA has no significant sequence similarities to known genes, redB has sequence similarity to invertebrate sarcoplasmic calcium-binding proteins. The mynD gene is closely related to type II myosin heavy-chain genes from many organisms and is one of a family of type II myosin genes in P. polycephalum. Our results indicate that many more red genes remain to be identified, some of which may play key roles in controlling plasmodium formation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002030050773

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072 Wound Healing Properties of Reconstituted Freeze-Dried Platelets (2005)

Ho, D. ; Moskowitz, K. ; Sum, R. ; [et al.]

Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.

Wound repair and regeneration 13 (2005), S. 0

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ISSN: 1524-475X

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: The use of fresh platelets has gained value in medicine as an essential part of wound treatments. This is not surprising since platelets contain a number of bioactive factors that contribute to the process of wound healing, such as platelet-derived growth factor (PDGF) and transforming growth factor (TGF). Fresh platelets’ short shelf life limits platelet-based therapies. If platelets can be stabilized in freeze-dried form (FDP) then long-term storage as well as pathogen inactivation methods become possible. Adlyfe and Oregon Freeze-Dry have been developing technology to stabilize freeze-dried human platelets that can be subjected to gamma irradiation and stored for a long duration. Upon reconstitution, irradiated FDP retained growth factors PDGF-AB, PDGF-BB, and TGF-B1 in quantities similar to fresh platelets as judged by capture ELISA. The rehydrated FDP promoted new DNA synthesis and cellular proliferation of primary human dermal fibroblasts and endothelial cells (HUVECs) similar to fresh platelets. The FDP also promoted remodeling of extracellular matrix by accelerating fibroblast-mediated contraction of collagen gels and stimulated HUVECs to undergo angiogenesis and form capillary structures in vitro. Therefore, we conclude that FDP and fresh platelets have comparable in vitro wound healing potential. Preclinical wound healing stud"ies in diabetic mice are under way and further development will allow FDP to be a safe and well-suited alternative to fresh platelets for wound healing applications.