ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Overexpression of tumour necrosis factor-α-converting enzyme in psoriasis (2005)

Kawaguchi, M. ; Mitsuhashi, Y. ; Kondo, S.

Oxford, UK : Blackwell Science Ltd

British journal of dermatology 152 (2005), S. 0

add to mindlist on the mindlist

Details

ISSN: 1365-2133

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Background Tumour necrosis factor (TNF)-α-converting enzyme (TACE) is a metalloproteinase-disintegrin that releases soluble TNF-α from cells by cleaving within the extracellular domain of membrane-bound pro-TNF-α. It was proposed that TNF-α is involved in the pathogenesis of psoriasis, and it is therefore suggested that TACE has important roles in psoriasis. However, it is unclear whether TACE is expressed in psoriatic tissue.Objectives To clarify whether TACE is expressed in psoriatic lesions and whether expression levels of TACE mRNA are increased in lesional compared with nonlesional psoriatic skin.Methods Skin biopsies were obtained from patients with psoriasis. We examined the expression of TACE in psoriatic tissues using a novel real-time quantitative reverse transcriptase–polymerase chain reaction method and immunohistochemical analysis.Results There was a significant rise in the level of TACE mRNA expression in lesional psoriatic skin compared with nonlesional skin in all patients. There was a statistically significant rise in the level of TACE mRNA expression in lesional psoriatic skin compared with nonlesional skin (mean ± SD TACE/glyceraldehyde-3-phosphate dehydrogenase ratio 0·031 ± 0·012 vs. 0·009 ± 0·002, P 〈 0·05). In lesional psoriatic skin, immunostaining with anti-TACE antibody was present throughout all layers of the epidermis. TACE immunostaining was found in the cytoplasm of keratinocytes. There was staining associated with blood vessels in the papillary dermis and perivascular inflammatory cells. In particular, mast cells showed strong staining. They contained numerous granules that were stained for TACE in the cytoplasm.Conclusions The findings of the present study suggest that elevation of TACE mRNA in psoriatic lesions is due to many cells, particularly mast cells, that function in lesional psoriatic skin as an important source of TNF-α and other cytokines.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2133.2005.06440.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Electrophoretic Behaviour of Rabbit Serum Amylase (1963)

BERK, J. E. ; KAWAGUCHI, M. ; ZEINEH, R. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 200 (1963), S. 572-573

add to mindlist on the mindlist

Details

ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Aliquots of 60 µl. of fresh serum from 7 rabbits were placed in duplicate on Whatman 3MM filter paper strips moistened with veronal buffer (pH. 8.6, ionic strength 0.075). Electrophoretic separations were performed in a horizontal cell at a constant potential of 80 V for 16 h. One ...