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2-Iodomelatonin prevents apoptosis of cerebellar granule neurons via inhibition of A-type transient outward K+ currents (2005)

Hu, Chang-Long ; Liu, Zheng ; Gao, Zhen-Yu ; [et al.]

Oxford, UK : Munksgaard International Publishers

Journal of pineal research 38 (2005), S. 0

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ISSN: 1600-079X

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: Compelling evidence indicates that excessive K+ efflux and intracellular K+ depletion are key early steps in apoptosis. Previously, we reported that apoptosis of cerebellar granular neurons induced by incubation under low K+ (5 mm) conditions was associated with an increase in delayed rectifier outward K+ current (IK) amplitude and caspase-3 activity. Moreover, the melatonin receptor antagonist 4P-PDOT abrogated the effects of 2-iodomelatonin on IK augmentation, caspase-3 activity and apoptosis. Here, we show that incubation under low K+/serum-free conditions for 6 hr led to a dramatic increase in the A-type transient outward K+ current (IA) (a 27% increase; n = 31); in addition, fluorescence staining showed that under these conditions, cell viability decreased by 30% compared with the control. Treatment with 2-iodomelatonin inhibited the IA amplitude recorded from control and apoptotic cells in a concentration-dependent manner and modified the IA channel activation kinetics of cells under control conditions. Moreover, 2-iodomelatonin increased the viability of cell undergoing apoptosis. Interestingly, 4P-PDOT did not abrogate the effect of 2-iodomelatonin on IA augmentation under these conditions; in the presence of 4P-PDOT (100 μm), 2-iodomelatonin reduced the average IA by 41 ± 4%, which was similar to the effect of 2-iodomelatonin alone. These results suggest that the neuroprotective effects of 2-idomelatonin are not only because of its antioxidant or receptor-activating properties, but rather that 2-iodomelatonin may inhibit IA channels by acting as a channel blocker.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1600-079X.2004.00174.x

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Functional Behaviour of Human Periodontal Ligament Cells around Various Dental Implants (2007)

Luo, Xiao Ting ; Gao, Zhen ; Yan, Shi Gui ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Key engineering materials Vol. 361-363 (Nov. 2007), p. 837-840

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ISSN: 1013-9826

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: In the present investigation, four titanium (Ti) surfaces of dental implants were comparedthrough in vitro systems. The surface roughness of Ti was measured by TR240 mobile surfaceroughmeter. The Ti implants were seeded with human periodontal ligament cells (hPLDCs) andmaintained for a period of 0-7 days. The adhesion, proliferation, and differentiation of hPLDCswere observed by using Cell morphology, cell counting and Osteocalcin (OC) immunofluorescentstaining. Results suggest that surface roughness of titanium favors hPDLCs behavior and improvescell adhesion, proliferation, and differentiation