ISSN:
1439-0264
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
We have applied a laser micro-dissection microscope followed by RT-PCR analysis of micro-dissected tissue to identify gene expression of various immune genes in the Atlantic salmon, Salmo salar. A major problem in teleost anatomy is the lack of specific cell markers to identify different leukocyte populations. For instance, the involvement of T-cells in inflammatory reactions is unknown. We used frozen sections from the gut of salmon fed to induce localized inflammatory reactions and micro-dissected regions showing proliferative changes. RNA was extracted from the micro-dissected specimens and subjected to quantitative RT-PCR using primers and TaqMan probes for T-cell receptor, MHC class II and elongation factor, which served as a house-keeping gene. The results showed expression of T-cell receptor, MHC class II and elongation factor. The expression of the elongation factor corresponded invariably to the area micro-dissected tissue. In conclusion, T-cells are present in teleost inflammatory reactions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1439-0264.2005.00669_61.x
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