Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
British journal of dermatology
153 (2005), S. 0
ISSN:
1365-2133
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Background A decoy strategy utilizing oligonucleotides (ODN) containing the specific binding sequence of a certain transcription factor has been developed and is considered to be a potential new class of antigene therapy. However, the application of this new therapeutic modality to skin diseases has not been fully documented.Objectives The aim of this work was to examine the effects of the nuclear factor (NF)-κB decoy ODN on UV-elicited skin change.Methods Mouse keratinocyte Pam 212 cells were transfected with NF-κB decoy ODN to examine the effects of the decoy ODN on ultraviolet (UV) B-induced apoptosis. Tape-stripped rat dorsal skin was treated with an ointment containing NF-κB decoy ODN for the examination of the in vivo impact of the decoy ODN on sunburned cell (SBC) formation and UVB erythema.Results NF-κB decoy ODN specifically induced apoptosis of Pam 212 cells and SBC formation was significantly enhanced by topical NF-κB decoy ODN ointment, while UV-induced erythema was not affected.Conclusions These data suggest that enhancement of UV-induced apoptosis by NF-κB decoy ODN may play a cancer-preventive role by further eliminating photodamaged keratinocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2133.2005.06969.x
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