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  • Electronic Resource  (5)
  • 2000-2004  (5)
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  • Electronic Resource  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 35 (2004), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Artificially induced maturation of 10 male eels Anguilla anguilla L. weighing 170 ± 30-3 g was followed for 6 wk with in vivo CT scanning in a repeated manner. Since testis tissue has a well defined X-ray density value, the volumetric development could be described. Parallel with this process, a strong decrease of the abdominal fat volume was measured and depicted with the applied surface smoothing method. As an affect of the hCG treatment (250 IU/week per eel) at the third wk, the testis could clearly be recognized on the images. From that time the average testis volume values of the 10 eels were 4,975, 6345, 11,583 and 14,553 mmJ respectively. At the sixth wk, the relative testis volume in the abdomen was 25%. In the fillet a characteristic shift (from -10 to -20 Hounsfield units) of the density histogram towards the fat interval was detected. The average density value of the fillet was found to be zero; this was supported furthermore by the histological analysis of the fillet (fat to muscle rate 0.35-0.40). On the basis of the above findings, the applied CT scanning method was proven to be suitable to follow in vivo the maturation process of male eels.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the World Aquaculture Society 35 (2004), S. 0 
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sexual maturation and sperm release were induced in farmed European eels Anguilla anguilla kept exclusively in fresh water by using two dosages of human chorion gonadotropin (100 International Unit (IU)-Group one and 250 IU/individual per week-Group two). Sperm release took over 13 wk in both groups. The quality of sperm was investigated on the eighth, ninth, and tenth wk. The average cell densities were 0.94 ± 0.4 × 1010 (Group one) and 0.93 to. ± 0.6 × 1010 (Group two) spermatozoa/mL. The estimated motility of eel sperm was 33, 55, and 49% on the eighth, ninth, and tenth wk of treatment, respectively. The estimated average motility of samples selected for cryopreservation was 73 ± 10%, while the post-thaw motility of cryopreserved samples was 36 ± 11%. The extender originally developed for common carp sperm crypreservation together with methanol as cryoprotectant was found suitable for the cryopreservation of European eel sperm.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The crude fat and protein content of the total body was estimated by X-ray computer tomography (CT) and determined by chemical analyses during a feeding experiment with sexually matured common carp. Between 21 and 35 serial scans were taken of altogether 41 fish and samples from the homogenized body were prepared for chemical analyses. Experimental fish with an average body weight of 1453 g originated from a commercial stock of mirror carp. Two feeding regimes (carp feed and carp feed+ad libitum maize) were applied for 57 days and followed by a fasting period of 27 days. Both feeding regimes significantly increased the crude fat content of the whole body that did not decrease during fasting in spite of high water temperature (22°C). No significant changes were observed in crude protein content. The variables used for producing the prediction equations were taken from the density values of the Hounsfield scale, on a range between −90 and +160, by summing the frequencies within each interval of 10 values. Whole body fat content could be estimated with R2=0.89−0.91 accuracy with the principal component analysis using data of all (seven) body regions and only dorsal fin region respectively. Adequate linear regression model could not be calculated by the same procedure for crude protein. Three-dimensional ‘volumetric’ estimation of fat tissue was also carried out on the basis of fat index showing high correlation with measured fat content. The changes of body composition of individual fish can be followed by sequential CT scanning.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Members of the ATP-dependent family of chromatin remodeling enzymes play key roles in the regulation of transcription, development, DNA repair and cell cycle control. We find that the remodeling activities of the ySWI/SNF, hSWI/SNF, xMi-2 and xACF complexes are nearly abolished by incorporation of ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The SIN domain within histones H3 and H4 is defined by a set of single amino acid substitutions that were initially identified as mutations that alleviate the transcriptional defects associated with inactivation of the SWI/SNF chromatin remodeling complex. Here we use recombinant histones to ...
    Type of Medium: Electronic Resource
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