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  • 1
    ISSN: 1432-2242
    Keywords: Key words Zea mays ; Pachytene chromosome ; Karyotype ; Repetitive DNA sequence ; Fluorescence in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A repetitive DNA sequence, ZmCR2.6c, was isolated from maize based on centromeric sequence CCS1 of the wild grass Brachypodium sylvaticum. ZmCR2.6c is 309 bp in length and shares 65% homology to bases 421–721 of the sorghum centromeric sequence pSau3A9. Fluorescence in situ hybridization (FISH) localized ZmCR2.6c to the primary constrictions of pachytene bivalents and to the stretched regions of MI/AI chromosomes, indicating that ZmCR2.6c is an important part of the centromere. Based on measurements of chromosome lengths and the positions of FISH signals of several cells, a pachytene karyotype was constructed for maize inbred line KYS. The karyotype agrees well with those derived from traditional analyses. Four classes of tandemly repeated sequences were mapped to the karyotype by FISH. Repeats 180 bp long are present in cytologically detectable knobs on 5L, 6S, 6L, 7L, and 9S, as well as at the termini and in the interstitial regions of many chromosomes not reported previously. A most interesting finding is the presence of 180-bp repeats in the NOR-secondary constriction. TR-1 elements co-exist with 180-bp repeats in the knob on 6S and form alone a small cluster in 4L. 26S and 5S rRNA genes are located in the NOR and at 2L.88, respectively. The combination of chromosome length, centromere position, and distribution of the tandem repeats allows all chromosomes to be identified unambiguously. The results presented form an important basis for using FISH for physical mapping and for investigating genome organization in maize.
    Type of Medium: Electronic Resource
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  • 2
    Book
    Book
    New Jersey [u.a.] :World Scientific,
    Title: Handbook of pattern recognition and computer vision /
    Contributer: Chen, C. H.
    Edition: 3. ed.
    Publisher: New Jersey [u.a.] :World Scientific,
    Year of publication: 2005
    Pages: IX, 639 S.
    ISBN: 981-256-105-6
    Type of Medium: Book
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  • 3
    Publication Date: 2020-03-20
    Language: English
    Type: article , doc-type:article
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  • 4
    ISSN: 1432-1211
    Keywords: Key words J chain ; Polymeric immunoglobulin ; Ontogeny ; Evolution ; Comparative immunology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The J chain is a component of polymeric immunoglobulin (Ig) molecules and may play an important role in their polymerization and the transport of polymeric Ig across epithelial cells. In this study, the primary structure of the chicken J chain was determined by sequencing cDNA clones. The cDNA had an open reading frame of 476 nucleotides encoding a putative protein of 158 amino acid residues including the signal sequence. The 3′ untranslated region consisted of 1216 nucleotides and a poly(A) tail. The deduced amino acid sequence of the chicken J chain had a high degree of homology to that of human, cow, rabbit, mouse, frog, and earthworm, with eight conserved Cys residues identical to the mammalian J chains. Northern blot hybridization performed with total RNA from various chicken tissues revealed high levels of J-chain mRNA expression in spleen, intestine, Harderian gland, and bursa of Fabricius, and low levels in the thymus. The J chain was expressed in the bursa as early as day 15 of embryogenesis. These data indicated that the chicken J-chain gene displays a high degree of homology with that of other species, and is expressed at an early stage of development of the chicken immune system.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 51 (June 2008), p. 71-78 
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Vacuum repair brazing of Inconel 939 using various ratios of DF3 and Rene 80 powdermixtures has been performed in this study. The porosity of the brazed zone is decreased as theamount of DF3 is increased in the powder mixture. At least four phases are observed in the brazedzone including chromium borides, sparse carbides, Ni-rich matrix and eutectic phases. Primarychromium borides are widely observed at the early stage of repair brazing. Dissolution of the Nifrom the Rene 80 powder into the molten braze results in isothermal solidification of the residualmelt. The chemical composition of the residual melt subsequently moves towards the lowesteutectic temperature, and the eutectic is finally formed in brazing. The oxidation resistance test isperformed at 1000oC. Extensive spalling of the oxide film begins at 144 h, and catastrophic failureof the oxide film is observed at 1272 h. The oxidation resistance of the brazed zone is significantlydegraded due to the formation of chromium borides in the brazed zone
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 47-50 (June 2008), p. 185-188 
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Notched tensile tests were performed to evaluate the presence of notch on the tensilestrength of Ti-6Al-6V-2Sn laser welds, which were post-weld aged at different temperatures.Ti-6Al-6V-2Sn laser welds showed notch brittleness except for the welds aged at/above 704 oC. Fineacicular α uniformly distributed in β matrix in the fusion zone accounted for the high hardness (Hv440) as compared with mill-annealed base metal. Overaging at/above 704 oC resulted in coarseningthe microstructures and decreased notch brittleness. The fracture features of the weld were stronglyaffected by the orientation of the solidified structures with respect to the crack growth direction
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 33-37 (Mar. 2008), p. 463-470 
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The multi-axial constitutive behavior of poled and unpoled ferroelectric ceramic PZT53is measured under different proportional tension-torsion loading and compression-torsion loadinguntil destroy. Deformation is measured using strain gauges. These results are used to map out“Switching” and “destroy” surfaces. The two surfaces are found to be a function of the polarization.Switching surface is similar to Mises and Tresca yield surface. Destroy behavior corresponds tomaximal principal normal stress rule. For poled PZT53, the effect of bias electric field on itsmultiaxial behavior has also been exploited.The results indicated that the “Switching” surfacedisappear with the increased bias electric. The ultimate goal is to develop an experimentallyvalidated micromechanics based phenomenological constitutive model for ferroelectric materials
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 114 (2001), S. 7338-7341 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: We show that the method of splitting the operator eε(T+V) to fourth order with purely positive coefficients produces excellent algorithms for solving the time-dependent Schrödinger equation. These algorithms require knowing the potential and the gradient of the potential. One fourth order algorithm only requires four fast Fourier transformations per iteration. In a one dimensional scattering problem, the fourth order error coefficients of these new algorithms are roughly 500 times smaller than fourth order algorithms with negative coefficient, such as those based on the traditional Forest–Ruth symplectic integrator. These algorithms can produce converged results of conventional second or fourth order algorithms using time steps 5 to 10 times as large. Iterating these positive coefficient algorithms to sixth order also produced better converged algorithms than iterating the Forest–Ruth algorithm to sixth order or using Yoshida's sixth order algorithm A directly. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 117 (2002), S. 1409-1415 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: We show that the method of factorizing the evolution operator to fourth order with purely positive coefficients, in conjunction with Suzuki's method of implementing time-ordering of operators, produces a new class of powerful algorithms for solving the Schrödinger equation with time-dependent potentials. When applied to the Walker–Preston model of a diatomic molecule in a strong laser field, these algorithms can have fourth order error coefficients that are three orders of magnitude smaller than the Forest–Ruth algorithm using the same number of fast Fourier transforms. Compared to the second order split-operator method, some of these algorithms can achieve comparable convergent accuracy at step sizes 50 times as large. Morever, we show that these algorithms belong to a one-parameter family of algorithms, and that the parameter can be further optimized for specific applications. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 116 (2002), S. 1211-1214 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Infrared spectra of CH stretches have been investigated on the surfaces of synthetic diamond crystallites (5, 100, and 700 nm in diameter) to provide insights into the mid-infrared emission bands of stardusts in interstellar media. While the spectrum of 5-nm diamonds fails to display the distinct 2835 cm−1 (or 3.53 μm) band, a remarkably good match of the absorption bands of larger diamond grains with the emission observed for the stars Elias 1 and HD 97048 is obtained. Suggested by this study, the nanodiamonds that can display such characteristic emission bands at 3.53 μm in these two interstellar systems have a size significantly larger than 5 nm. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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