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  • 1
    Electronic Resource
    Electronic Resource
    Evidence that phosphorylase kinase exhibits phosphatidylinositol kinase activity (1986)
    s.l. : American Chemical Society
    Biochemistry 25 (1986), S. 3867-3874 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00361a019
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  • 2
    Electronic Resource
    Electronic Resource
    Differential mRNA Expression of the Two Mineralocorticoid Receptor Splice Variants Within the Human Brain: Structure Analysis of their Different DNA Binding Domains (2000)
    Oxford, UK : Blackwell Science Ltd
    Journal of neuroendocrinology 12 (2000), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In human brain tissue, cortisol action, at basal concentrations, is mediated by the mineralocorticoid receptor (MR). An in-frame insertion of 12 bp in the MR-DNA-binding domain due to alternative splice site usage between exons 3 and 4 results in an MR mRNA splice variant (MR+4) encoding a receptor protein with four additional amino acids compared to the wild-type MR protein. To elucidate the questions of sex, age, and/or tissue dependent differences of the relative amount of the two mRNA subtypes, we examined 131 fresh human brain tissue samples from temporal and frontal lobe or hippocampus. One hundred and twenty samples were obtained from patients with epilepsy and 11 samples from patients with brain tumours. A small but signi®cant difference of the MR+4 mRNA splice variant proportions in cortex (9.5t0.8%) and subcortical white matter (6.6t0.7%) of the temporal lobe could be detected, indicating differential MR splice variant expression within these brain areas. Moreover, the splice variant ratios in samples of the temporal lobe cortex collected from patients with epilepsy differed from samples of patients with brain tumours. These data point to an altered expression of the MR splice variants in epilepsy, and strengthen the supposition of a tissue speci®c alternative splicing of the MR mRNA. The frequent occurence of the MR+4 transcript raises the question of its functional signi®cance. For this reason, an MR+4 DNA-binding-domain structure model was generated by computer-based homology modelling based on the known glucocorticoid receptor structure. The data obtained revealed no distorting effect of the inserted four amino acids on the adjacent secondary structures, thereby suggesting that both zinc ®ngers retain their function. The resulting structure of the MR+4 model leads to the supposition that the receptor retains its function. Moreover, databank analysis with respect to this kind of steroid receptor variation and our own sequence data of the closely related progesterone receptor sustained the hypothesis that only corticosteroid receptors were affected by this alternative splicing event.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2826.2000.00535.x
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  • 3
    Electronic Resource
    Electronic Resource
    Carbonic anhydrase and C4 photosynthesis: a transgenic analysis (2004)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 27 (2004), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Carbonic anhydrase (CA, EC 4.2.1.1) catalyses the first reaction in the C4 photosynthetic pathway, the conversion of atmospheric CO2 to bicarbonate in the mesophyll cytosol. To examine the importance of the enzyme to the functioning of the C4 photosynthetic pathway, Flaveria bidentis (L.) Kuntze, a C4 dicot, was genetically transformed with an antisense construct in which the cDNA encoding a putative cytosolic CA (CA3) was placed under the control of a constitutive promoter. Some of the primary transformants had impaired CO2 assimilation rates and required high CO2 for growth. The T1 progeny of four primary transformants were used to examine the quantitative relationship between leaf CA activity and CO2 assimilation rate. CA activity was determined in leaf extracts with a mass spectrometric technique that measured the rate of 18O exchange from doubly labelled 13C18O2. Steady-state CO2 assimilation rates were unaffected by a decrease in CA activity until CA activity was less than 20% of wild type when they decreased steeply. Transformants with less than 10% of wild-type CA activity had very low CO2 assimilation rates and grew poorly at ambient CO2 partial pressure. Reduction in CA activity also increased the CO2 partial pressure required to saturate CO2 assimilation rates. The present data show that CA activity is essential for the functioning of the C4 photosynthetic pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.2003.01157.x
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  • 4
    Electronic Resource
    Electronic Resource
    Magnocellular Dendrites: Prototypic Receiver/Transmitters (2004)
    Oxford, UK : Blackwell Science Ltd
    Journal of neuroendocrinology 16 (2004), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The hypothalamic magnocellular neurones of the supraoptic and paraventricular nuclei of mammals are among the best understood of all peptidergic neurones, and therefore serve as a model for understanding the functions of other peptidergic neurones. The release of vasopressin- and oxytocin-containing neurosecretory vesicles from their dendrites was first established 15 years ago. This local release is now known to have many functions, including controlling the interactions between oxytocin neurones and their surrounding glia, and facilitating and inhibiting the electrical activation of the neurones. Technical advances now permit dynamic analysis of dendritic release. Here, we review recent studies that focus on the conditional priming of dendritic peptide release by peptide-induced liberation of intracellular calcium, and the role of dendritic protein synthesis in the dendritic peptide release and the control of receptive properties of the neurones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0953-8194.2004.01182.x
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  • 5
    Electronic Resource
    Electronic Resource
    Vasopressin Regulation of Noradrenaline Release Within the Supraoptic Nucleus (2000)
    Oxford, UK : Blackwell Science Ltd
    Journal of neuroendocrinology 12 (2000), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effect of electrically evoked dendritic vasopressin release on noradrenaline release into the hypothalamic supraoptic nucleus was assessed by in vivo microdialysis in conjunction with high pressure liquid chromatography and electrochemical detection. Electrical activation of magnocellular supraoptic neurones by stimulation of their axons at the level of the neural lobe significantly increased noradrenaline release into the nucleus (2.5-fold, P〈0.03). This increase was completely blocked by administration of a nonpeptide vasopressin V1a receptor antagonist via the microdialysis probe. These data suggest that dendritically released vasopressin facilitates noradrenaline release into the hypothalamic nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2826.2000.00516.x
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  • 6
    Electronic Resource
    Electronic Resource
    Mechanical strain enhances proteoglycan message in fibroblasts from asthmatic subjects (2004)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 34 (2004), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Remodelling of the asthmatic airway includes increased deposition of proteoglycan (PG) molecules. One of the stimuli driving airway remodelling may be excessive mechanical stimulation.Objective We hypothesized that fibroblasts from asthmatic patients would respond to excessive mechanical strain with up-regulation of message for PGs.Methods We obtained fibroblasts from asthmatic patients (AF) and normal volunteers (NF) using endobronchial biopsy. Cells were maintained in culture until the fifth passage and then grown on a flexible collagen-coated membrane. Using the Flexercell device, cells were then subjected to cyclic stretch at 30% amplitude at 1 Hz for 24 h. Control cells were unstrained. Total RNA was extracted from the cell layer and quantitative RT-PCR performed for decorin, lumican and versican mRNA.Results In unstrained cells, the expression of decorin mRNA was greater in AF than NF. With strain, NF showed increased expression of versican mRNA and AF showed increased expression of versican and decorin mRNA. The relative increase in versican mRNA expression with strain was greater in AF than NF.Conclusions These data support the hypothesis that proteoglycan message is increased in asthmatic fibroblasts subject to mechanical strain. This finding has implications for the mechanisms governing airway wall remodelling in asthma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.2004.01980.x
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  • 7
    Electronic Resource
    Electronic Resource
    Die Diagnose sehr früher arteriosklerotischer Gefäßwandveränderungen mit Hilfe der Duplexsonographie (1989)
    Springer
    Journal of molecular medicine 67 (1989), S. 442-446 
    Details
    ISSN: 1432-1440
    Keywords: Duplex Sonographie ; early atherosclerotic changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The technical evolution of ultrasonic equipment provides a high resolution imaging analysis of the vessel wall and thereby offers new possibilities in diagnosing very early atherosclerotic changes. The typical B-mode image in human and animal arteries shows parallel wall contures enclosing a hypoechcic space. In this study in Vitro-and in Vivo-experiments in rabbit aortas document the distance between these contures correlating histologically with a high cholesterol diet caused a broadening of the hypoechoic space in the rabbit aortic vessel wall. The data demonstrate that high resolution Duplex Sonography is a useful noninvasive approach for the detection of very early atherosclerotic changes in arterial vessel walls in a stage before plaques can be identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01725140
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  • 8
    Electronic Resource
    Electronic Resource
    Einsatz der Kryotechnologie in der Behandlung männlicher Infertilität (2000)
    Springer
    Der Gynäkologe 33 (2000), S. 130-134 
    Details
    ISSN: 1433-0393
    Keywords: Schlüsselwörter Kryokonservierung ; Heterologe Insemination ; Intrazytoplasmatische Spermieninjektion ; Samenbank ; Key words Cryopreservation ; Donor insemination ; Sperm banking ; Intracytoplasmic sperm injection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract The cryopreservation technique has a great impact in the treatment of male infertility. Especially in males undergoing chemotherapy for oncological diseases, cryopreservation of sperm samples may help to preserve the chance to father a child after completion of the oncological treatment. Furthermore, cryopreservation is necessary to optimize the screening of sperm donors for insemination. The cryopreservation of testicular samples or epididymal aspirates in patients suffering from azoospermia helps to avoid unnecessary hormonal stimulation procedures in the female partner. Only in cases in which fertilizable sperms are present should an intracytoplasmic sperm injection cycle be planned. The success rates of treatment using frozen or non-frozen sperm samples seem to be the same, especially since the introduction of intracytoplasmic sperm injection. This helps to overcome the lower fertilizing capacity of sperms following cryopreservation due to loss of quality.
    Notes: Zusammenfassung Die Kryokonservierung hat im Rahmen der männlichen Infertilität Bedeutung insbesondere im Rahmen der Bewahrung der Fertilität bei onkologischen Erkrankungen vor Durchführung einer Chemotherapie, sowie bei der heterologen Insemination, um die infektiologische Testung von Spender und Ejakulat zu ermöglichen. Ferner hat sich das Konzept etabliert bei Vorliegen einer Azoospermie zunächst durch eine Hodenbiopsie bzw. epididymale Spermienaspiration das Vorhandensein fertilisierungsfähiger Spermien zu evaluieren, bevor die Partnerin hormonell stimuliert wird. Auch hier müssen Hodengewebe und Nebenhodenaspirat kryokonserviert werden. Die Erfolge der mit einer Kryokonservierung kombinierten Techniken zur Behandlung der männlichen Fertilität sind gut. Nachteile sind – insbesondere durch die Einführung der intrazytoplasmatischen Spermieninjektion – gegenüber der Verwendung nichtkryokonservierter Spermien offensichtlich nicht vorhanden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001290050022
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  • 9
    facet.materialart.
    Unknown
    Die Personennamen in Hans Jacob Christoffel von Grimmelshausens Simplicius Simplicissimus. Zu einem komplexen Fall literarischer Onomastik (1988)
    Groningen, Netherlands : Periodicals Archive Online (PAO)
    Neophilologus. 72:1 (1988:Jan.) 66 
    Details
    ISSN: 0028-2677
    Topics: Linguistics and Literary Studies
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:5262-1988-072-01-000007
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  • 10
    facet.materialart.
    Unknown
    Feierlichkeiten zum 200. Geburtstag von JOHANN ANDREAS SCHMELLER, 18.-21. Juli 1985 in Tirschenreuth (Oberpfalz), mit Verleihung des JOHANN-ANDREAS-SCHMELLER-Preises 1985 (1986)
    Wiesbaden, etc. : Periodicals Archive Online (PAO)
    Zeitschrift für Dialektologie und Linguistik. 53:2 (1986) 206 
    Details
    ISSN: 0044-1449
    Topics: Linguistics and Literary Studies
    Notes: Berichte
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:0059-1986-053-02-000009
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