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  • 2000-2004  (7)
  • 1975-1979  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    CVD Mullite Coatings in High-Temperature, High-Pressure Air–H2O (2000)
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 83 (2000), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Crystalline mullite was deposited by chemical vapor deposition (CVD) onto SiC/SiC composites overlaid with CVD SiC. Specimens were exposed to isothermal oxidation tests in high-pressure air + H2O at 1200°C. Unprotected CVD SiC formed silica scales with a dense amorphous inner layer and a thick, porous, outer layer of cristobalite. Thin coatings (∼2 μm) of dense CVD mullite effectively suppressed the rapid oxidation of CVD SiC. No microstructural evidence of mullite volatility was observed under these temperature, pressure, and low-flow-rate conditions. Results of this preliminary study indicate that dense, crystalline, high-purity CVD mullite is stable and protective in low-velocity, high-pressure, moisture-containing environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1151-2916.2000.tb01250.x
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  • 2
    Electronic Resource
    Electronic Resource
    Electrical and neurotransmitter activity of mature neurons derived from mouse embryonic stem cells by Sox-1 lineage selection and directed differentiation (2004)
    Oxford, UK : Blackwell Science Ltd
    European journal of neuroscience 20 (2004), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Sx1TV2/16C is a mouse embryonic stem (ES) cell line in which one copy of the Sox1 gene, an early neuroectodermal marker, has been targeted with a neomycin (G418) selection cassette. A combination of directed differentiation with retinoic acid and G418 selection results in an enriched neural stem cell population that can be further differentiated into neurons. After 6–7 days post-plating (D6–7PP) most neurons readily fired tetrodotoxin (TTX)-sensitive action potentials due to the expression of TTX-sensitive Na+ and tetraethylammonium (TEA)-sensitive K+ channels. Neurons reached their maximal cell capacitance after D6–7PP; however, ion channel expression continued until at least D21PP. The percentage of cells receiving spontaneous synaptic currents (s.s.c.) increased with days in culture until 100% of cells received a synaptic input by D20PP. Spontaneous synaptic currents were reduced in amplitude and frequency by TTX, or upon exposure to a Ca2+-free, 2.5 mm Mg2+ saline. S.s.c. of rapid decay time constants were preferentially blocked by the nonNMDA glutamatergic receptor antagonists CNQX or NBQX. Ca2+ levels within ES cell-derived neurons increased in response to glutamate receptor agonists l-glutamate, AMPA, N-methyl-d-aspartate (NMDA) and kainic acid and to acetylcholine, ATP and dopamine. ES cell-derived neurons also generated cationic and Cl–-selective currents in response to NMDA and glycine or GABA, respectively. It was concluded that ES-derived neurons fire action potentials, receive excitatory and inhibitory synaptic input and respond to various neurotransmitters in a manner akin to primary central neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-9568.2004.03782.x
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  • 3
    Electronic Resource
    Electronic Resource
    A ComGA-dependent checkpoint limits growth during the escape from competence (2001)
    Oxford, UK : Blackwell Science, Ltd
    Molecular microbiology 40 (2001), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In Bacillus subtilis, competence for transformation develops in 5–10% of the cells in a stationary phase culture. These cells exhibit a prolonged lag in the resumption of growth and cell division during the escape from competence. To better understand the basis of this lag, we have characterized competent cultures microscopically. To distinguish the minority of competent cells, a translational fusion between ComK, the competence transcription factor, and the green fluorescent protein (GFP) was used as a marker. Only 5–10% of the cells in a competent culture were fluorescent, indicating that ComK synthesis is an all or nothing event. To validate the identification of competent cells, we demonstrated the coincident expression of comEA, a late competence gene, and comK-gfp. Competent cells resemble stationary phase cells; the majority are single (not in chains), contain single nucleoids, and rarely contain FtsZ rings. Upon dilution into fresh medium, competent cells maintain this appearance for about 2 h. In contrast, the majority of non-competent cells rapidly resume growth, exhibiting chaining, nuclear division and FtsZ-ring formation. The late competence protein ComGA is required for the competence-related block in chromosome replication and cell division. In the competent cells of a comGA mutant culture, chromosomal replication and FtsZ-ring formation were no longer blocked, although competent comGA mutant cells were abnormal in appearance. It is likely that one role for ComGA is to prevent growth, chromosome replication and cell division until ComK can be eliminated by degradation. A mutation in the ATP-binding site of comGA inactivated the protein for transformation but did not prevent it from inhibiting DNA replication and cell division. The buoyant density difference between competent and non-competent cells depends on the competence-specific growth arrest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2001.02363.x
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  • 4
    Electronic Resource
    Electronic Resource
    Phase Formation and Stability in Reactively Sputter Deposited Yttria-Stabilized Zirconia Coatings (2001)
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 84 (2001), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Yttria-stabilized zirconia (YSZ) coatings were produced by reactively cosputtering metallic zirconium and yttrium targets in an argon and oxygen plasma using a system with multiple magnetron sputtering sources. Coating crystal structure and phase stability, as functions of Y2O3 content, substrate bias, and annealing temperature, were investigated by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Results demonstrated that highly (111)-oriented tetragonal and cubic zirconia structures were formed in 2 and 4.5 mol% Y2O3 coatings, respectively, when the coatings were grown with an applied substrate bias. Conversely, coatings deposited with no substrate bias had random tetragonal and cubic structures. XRD analysis of annealed coatings showed that the cubic zirconia in 4.5 mol% Y2O3 coatings exhibited structural stability at temperatures up to 1200°C. Transformation of the tetragonal to monoclinic phase occurred in 2 mol% Y2O3 coating during high-temperature annealing, with the fraction of transformation dependent on bias potential and annealing temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1151-2916.2001.tb00770.x
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  • 5
    Electronic Resource
    Electronic Resource
    Optimization of Chemical Vapor Deposition Parameters for Fabrication of Oxidation-Resistant Mullite Coatings on Silicon Nitride (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Ceramic Society 87 (2004), S. 0 
    Details
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Fabrication of mullite (3Al2O3·2SiO2) coatings by chemical vapor deposition (CVD) using AlCl3–SiCl4–H2–CO2 gas mixtures was studied. The resultant CVD mullite coating microstructures were sensitive to gas-phase composition and deposition temperature. Chemical thermodynamic calculations performed on the AlCl3–SiCl4–H2–CO2 system were used to predict an equilibrium CVD phase diagram. Results from the thermodynamic analysis, process optimization, and effects of various process parameters on coating morphology are discussed. Dense, adherent crystalline CVD mullite coatings ∼2 μm thick were successfully grown on Si3N4 substrates at 1000°C and 10.7 kPa total pressure. The resultant coatings were 001 textured and contained well-faceted grains ∼0.3–0.5 μm in size.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1151-2916.2004.tb07491.x
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  • 6
    Electronic Resource
    Electronic Resource
    Culture of human malaria parasites Plasmodium falciparum (1976)
    [s.l.] : Nature Publishing Group
    Nature 263 (1976), S. 767-769 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Parasites for culture were obtained from a splenectom-ised chimpanzee (Pan troglodytes) inoculated with a chimpanzee-adapted isolate of the Malayan Camp strain of P. falciparum12. Nine days after inoculation 52% of its erythrocytes were parasitised with ring forms and 6% with schizonts. A sample ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/263767a0
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Specific basophil hypersensitivity induced by skin testing and transferred using immune serum (1975)
    [s.l.] : Nature Publishing Group
    Nature 256 (1975), S. 52-54 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Two separate preparations of KLH (Pacific Bio-Marine, Venice, California) were used for these studies. Hartley strain albino guinea pigs (250-300 g) were bred at the Division of Animal Care at Yale University School of Medicine. On day 0 animals were flank skin tested by intradermal injection of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/256052a0
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  • 8
    Electronic Resource
    Electronic Resource
    A proteomic view of the Plasmodium falciparum life cycle (2002)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 419 (2002), S. 520-526 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The completion of the Plasmodium falciparum clone 3D7 genome provides a basis on which to conduct comparative proteomics studies of this human pathogen. Here, we applied a high-throughput proteomics approach to identify new potential drug and vaccine targets and to better understand the biology of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature01107
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  • 9
    Electronic Resource
    Electronic Resource
    Sterilization of boll weevil pupae with fractionated doses of gamma irradiation (1977)
    Springer
    Entomologia experimentalis et applicata 21 (1977), S. 57-62 
    Details
    ISSN: 1570-7458
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Durch fraktionierte Dosen von insgesamt 6250 und 8000 rads einer Gammabestrahlung (25 bzw. 32 Teilbehandlungen zu 250 rads mit 4 h-Intervallen) wurden Puppen des Baumwollkapselkäfers, Anthonomus grandis Boheman, in beiden Geschlechtern sexuell sterilisiert. Die Sterblichkeit, 5 d nach der Behandlung, betrug bei den so behandelten Männchen bei 6250 rads 14%, bei 8000 rads 27% im Vergleich zu 46%, wenn eine äquivalente akute Dosis bei frisch geschlüpften Käfern angewendet wurde. Die Pheromonproduktion der Männchen in den ersten 4 d erreichte bei 6250 rads-Bestrahlung 1/3 derjenigen der Kontrolltiere; 5–11 d nach der Behandlung war kein Unterschied. Dieses Verfahren eignet sich für die Massensterilisation von Kapselkäfern, wie sie bei einem Ausrottungsprogramm nötig ist. Diese Technik ist anwendbar auf andere Insekten, die gegenüber akuten Dosen hochempfindlich sind.
    Notes: Abstract Fractionated doses of 6,250–8,000 rads of gamma irradiation when administered to pupae of the boll weevil, Anthonomus grandis Boh., sexually sterilized both sexes. Mortality of males thus treated with 6,250 and 8,000 rads via fractionation was 14% and 27% respectively, by 5 days posttreatment compared with 46% mortality when an equivalent acute dose was administered to newly emerged adults. Pheromone production of males irradiated at 6,250 rads was one-third that of the control for the first 4 days, but equal that of the control during 5–11 days posttreatment. This procedure lends itself to the large-scale sterilization of weevils needed in an eradication program. This technique is applicable to other insects that are highly susceptible to acute doses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300341
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  • 10
    Electronic Resource
    Electronic Resource
    A new antisickling agent: In vitro studies of its effect on S/S erthrocytes and on hemoglobin S (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 804-806 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 2-{benzoylamino} pyridinium benzoate (BAPB) has exhibited an antisickling effect with homozygous S/S erythrocytes in vitro. This study suggests that BAPB prevents sicklin by inhibiting the gelation of hemoglobin S.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01947338
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