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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of the ftsZ cell division gene of Neisseria gonorrhoeae: expression in Escherichia coli and N. gonorrhoeae (2000)
    Springer
    Archives of microbiology 173 (2000), S. 10-20 
    Details
    ISSN: 1432-072X
    Keywords: Key words Bacteria ; Cloning ; FtsZ ; Cell division ; ¶Neisseria gonorrhoeae ; Localization ; Expression ; Green fluorescent protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We cloned the cell division gene ftsZ of the gram-negative coccus Neisseria gonorrhoeae (Ng) strain CH811, characterized it genetically and phenotypically, and studied its localization in N. gonorrhoeae and Escherichia coli (Ec). The 1,179-bp ORF of ftsZ Ng encodes a protein with a predicted molecular mass of 41.5 kDa. Protein sequence alignments indicate that FtsZNg is similar to other FtsZ proteins and contains the conserved GTP binding motif. FtsZ homologues were identified in several N. gonorrhoeae strains and in Neisseria lactamica, Neisseria sicca, Neisseria polysaccharae and Neisseria cinerea either by Western blot or by PCR-Southern blot analysis. Attempts to inactivate the ftsZ Ng on the chromosome failed, indicating that it is essential for gonococcal growth. FtsZNg was synthesized in an in vitro transcription/translation system and was shown to be 43 kDa, the same size as in Western blots. Expression of the ftsZ Ng gene from nongonococcal promoters resulted in a filamentous phenotype in E. coli. Under controlled expression, the FtsZNg-GFP fusion protein localized at the mid-cell division site in E. coli. E. coli expressing high levels of the FtsZNg-GFP fusion protein formed filaments and exhibited different fluorescent structures including helices, spiral tubules extending from pole to pole, and regularly spaced dots or bands that did not localize ¶at the middle of the cell. Expression of the FtsZNg-GFP fusion protein in N. gonorrhoeae resulted in abnormal cell division as shown by electron microscopy. FtsZNg-GFP fusions were also expressed in a gonococcal background using a unique shuttle vector.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050002
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  • 2
    Electronic Resource
    Electronic Resource
    Reproductive failure in women living in iodine deficient areas of West Africa (2000)
    Oxford, UK : Blackwell Publishing Ltd
    BJOG 107 (2000), S. 0 
    Details
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objectives To investigate the association between iodine status and reproductive failure in a population of West African women.Design Epidemiological survey on iodine deficiency disorders carried out in 1996–1997.Setting The iodine deficient areas of Senegal (Casamance and Senegal Oriental).Population Four thousand nine hundred and eighty women, aged 10 to 50, of whom 1544 adolescent and 462 pregnant women were examined for thyroid size and urinary iodine excretion. Their iodine status was associated with their fertility rate and reproductive failures.Results Reproductive failure (defined as repeated miscarriages and stillbirth) was associated with low iodine status, with severe iodine deficiency increasing the risk. Poor nutritional status and illiteracy had a significant effect on the outcome of pregnancy: underweight women had a fourfold higher risk, and those who were illiterate an eightfold higher risk, of failed pregnancy, compared with nutritionally healthy, literature women.Conclusions These findings emphasise the need to implement an effective iodine supplementation programme targeted at young and pregnant women in this area of western Africa. They also emphasise the importance of improving the nutritional status of young girls and the crucial role played by education in the prevention of reproductive failure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-0528.2000.tb13305.x
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  • 3
    Electronic Resource
    Electronic Resource
    The prescribing of acid suppressants prior to the endoscopic diagnosis of Barrett’s oesophagus and oesophagitis (2001)
    Oxford UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 15 (2001), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: There has been a dramatic rise in incidences of Barrett’s oesophagus and oesophageal adenocarcinoma. It has been suggested that the introduction and use of acid suppression therapy may be a factor in the rising incidences of Barrett’s oesophagus and oesophageal adenocarcinoma.〈section xml:id="abs1-2"〉〈title type="main"〉Methods:This was a record linkage study, using a prescribing database and an endoscopy database. Patients who had undergone their first endoscopy during the period 1992–1995 and received the diagnosis of Barrett’s oesophagus or oesophagitis were identified. The prescribing of acid suppressants was compared for the 3 years prior to endoscopy, between those with Barrett’s oesophagus and those with oesophagitis.〈section xml:id="abs1-3"〉〈title type="main"〉Results:There was no significant difference between the Barrett’s patients and the oesophagitis patients in the proportion that had been exposed to acid suppression therapy (53.4% vs. 51.7%, P=0.704). The mean number of days of prescribing among those who had been exposed to acid suppression therapy was higher in the Barrett’s group (340.5 vs. 237.0 days, P=0.001).〈section xml:id="abs1-4"〉〈title type="main"〉Conclusions:Patients with Barrett’s oesophagus have received more acid suppressant therapy prior to diagnosis. The reasons for this are not clear. However, 46.6% of Barrett’s patients have not been exposed to acid suppressant therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.2001.00914.x
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