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  • 1
    Electronic Resource
    Electronic Resource
    Threshold collision-induced dissociation of anionic copper clusters and copper cluster monocarbonyls (2000)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 112 (2000), S. 1713-1720 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The threshold collision-induced dissociation method is applied to study the fragmentation patterns and to measure the dissociation energies of small anionic copper clusters (Cun−, n=2–8) and their monocarbonyls (CunCO−, n=3–7). For the bare clusters, the main reaction channels are loss of an atom and loss of a dimer. For the copper cluster monocarbonyls, the main channel is loss of CO. Dissociation energies for the loss of an atom from bare copper cluster anions, D0(Cun−1−–Cu), show even–odd alternation. The species with the highest dissociation energy, Cu7−, and the highest carbonyl desorption energy, Cu5CO−, have eight valence electrons, consistent with closed shells in the jellium model. Bond energies are compared with theoretical models. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.480736
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  • 2
    Electronic Resource
    Electronic Resource
    Collisional activation of the endoergic hydrogen atom transfer reaction S−(2P)+H2→SH−+H (2000)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 112 (2000), S. 4579-4590 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Guided ion beam tandem mass spectrometry and ab initio calculations are used to investigate the reactions of S−(2P) with H2 and D2. The hydrogen atom transfer reaction to form SH− or SD− is observed at collision energies above the reaction endoergicity. Coupled-cluster theory calculations indicate that the hydrogen abstraction reaction occurs with no barrier along the C∞v collinear reaction path. The threshold energy yields the bond dissociation energy D0(S−−H)=373.1 ± 4.3 kJ/mol. Using literature values for electron affinities, we derive D0(S–H)=350.0±4.3 kJ/mol, in agreement with previous values. We also obtain gas phase acidities for H2S and SH, ΔacidH298(H2S)=1467.9±4.3 kJ/mol and ΔacidH298(SH)=1465.2±4.3 kJ/mol. The competition between hydrogen atom transfer and the exoergic associative detachment reaction forming SH2+e− is discussed. Calculations reveal that the transition state for insertion leading to associative detachment is a bent asymmetric SHH− species with an energy of 47 kJ/mol relative to S−+H2 reactants. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.481016
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of a Mycobacterium sp. in rockfish, Sebastes alutus (Gilbert) and Sebastes reedi (Westrheim & Tsuyuki), using rDNA sequences (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 26 (2003), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.2003.00451.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ichthyophonus and Mycobacterium-like bacterial infections in commercially-important rockfish, Sebastes spp., in the eastern North Pacific Ocean (2001)
    Oxford UK : Blackwell Science Ltd
    Journal of fish diseases 24 (2001), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.2001.00313.x
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  • 5
    Electronic Resource
    Electronic Resource
    Ribosomal DNA sequences indicate isolated populations of Ichthyophonus hoferi in geographic sympatry in the north-eastern Pacific Ocean (2002)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 25 (2002), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Infections of Ichthyophonus hoferi, a cosmopolitan parasite of marine fish, have recently been reported in rockfish, Sebastes spp., from the north-eastern Pacific. Because I. hoferi also infects Pacific herring, Clupea pallasi Valenciennes, and salmonids in this region, we wanted to determine if Ichthyophonus parasites from rockfishes, Pacific herring and chinook salmon, Oncorhynchus tshawytscha (Walbaum), were the same. Small subunit ribosomal deoxyribonucleic acid sequence data revealed two haplotypes that were fixed among host species in geographic sympatry, one from rockfish and the other from both Pacific herring and salmon. These isolated populations of Ichthyophonus could be part of the same species that are ecologically separated because of host behaviours, or they could be distinct species that are host specific. Dietary patterns of the hosts indicate that ecological separation among hosts is possible, but the presence of distinct species may better explain the observed Ichthyophonus haplotype association with host species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.2002.00404.x
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  • 6
    Electronic Resource
    Electronic Resource
    Transmission and tissue distribution of Pseudoloma neurophilia (Microsporidia) of zebrafish, Danio rerio (Hamilton) (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 26 (2003), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.2003.00467.x
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  • 7
    Electronic Resource
    Electronic Resource
    Composition of foods using microwave dielectric spectra (2000)
    Springer
    European food research and technology 210 (2000), S. 359-366 
    Details
    ISSN: 1438-2385
    Keywords: Key words Dielectrics ; Foods ; Composition ; Polyphosphate ; Added water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  As part of a European Union project (FAIR CT97 3020) microwave dielectric spectra of processed prawns (Pandalus borealis), cod (Gadus morhua), pork and chicken were measured and the data transformed using the statistical method of principal component analysis. The principal components were regressed (principal component regression) against various compositional variables, notably added water, protein, water, total phosphorus and NaCl and the calibration was validated using the method of internal cross-validation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050564
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  • 8
    Electronic Resource
    Electronic Resource
    Determination of prior treatment of fish and fish products using microwave dielectric spectra (2000)
    Springer
    European food research and technology 210 (2000), S. 427-433 
    Details
    ISSN: 1438-2385
    Keywords: Key words Dielectrics ; Fish ; Added water ; Polyphosphate ; Freezing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Microwave dielectric spectra of fish products [processed prawns (Pandalus borealis), cod (Gadus morhua), haddock (Melanogrammus aeglefinus) and saithe (Pollachus virens)] were transformed using the statistical method of principal component analysis. The principal components obtained were used as descriptors to construct discriminant functions for the separation of the samples into groups, e.g. added water or no added water, polyphosphate or no polyphosphate. The method was validated using internal cross-validation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002170050576
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  • 9
    Electronic Resource
    Electronic Resource
    Structure and Organization of the rDNA Intergenic Spacer in Lake Trout (Salvelinus namaycush) (2000)
    Springer
    Chromosome research 8 (2000), S. 5-16 
    Details
    ISSN: 1573-6849
    Keywords: intergenic spacer (IGS) ; lake trout ; ribosomal DNA ; salmonid ; Salvelinus namaycush
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total-genomic cosmid library was created to isolate complete copies of the rDNA cistron of lake trout (Salvelinus namaycush) in order to study the structure and organization of the intergenic spacer (IGS) in this species. A total of 60 rDNA- positive clones (average inserts 〉 25 kb) was recovered by screening the library with a rDNA-specific probe. Positive clones were assayed for the presence of the two internal rDNA spacers (ITS-1 and ITS-2) and the entire IGS fragment was successfully amplified from 42 clones by PCR. Length of the IGS fragments ranged from 9.4 to 17.8 kb. Comparative restriction mapping of the IGS–PCR products of several clones indicated two regions of extensive length variation surrounding a central region with sequence conservation. DNA sequence analysis was used to investigate the molecular basis of the IGS length variation and focused on identifying the region responsible for this variation. Over 9 kb of DNA sequence was obtained for one clone (A1) with a total IGS length of approximately 12.4 kb. Sequence of a conserved central region contained two open reading frames and a number of short direct repeats. Length variation in the IGS was determined by RFLP to result from differences in the number of copies of repetitive DNA sequences. These included an 89-bp tandem repeat (α repeats), an 82-bp element (β repeats), a 168–177-bp element (S repeats), and a 179–201-bp element (δ repeats). Overall nucleotide composition of the IGS was biased towards A and T (%GC = 47.4). Maintenance of discrete rDNA-length variants in lake trout suggests that the rate of gene conversion is insufficient to produce homogeneous copies across the genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009214800251
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  • 10
    Electronic Resource
    Electronic Resource
    Localization of Repetitive DNAs to Zebrafish (Danio rerio) Chromosomes by Fluorescence in situ Hybridization (FISH) (2000)
    Springer
    Chromosome research 8 (2000), S. 27-35 
    Details
    ISSN: 1573-6849
    Keywords: cytogenetics ; fluorescence in-situ hybridization ; repetitive DNA ; zebrafish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome of the zebrafish, Danio rerio, contains two major classes of tandem repetitive elements (AT-rich and GC-rich). The AT-rich repeats can be further subdivided into two subgroups which differ by about 10% of 185 bp in the repeating unit. The chromosomal location of these sequences and the moderately repetitive 5S rDNA sequences was determined in two diploid zebrafish cell lines using in-situ hybridization with fluorochrome-labeled probes. The AT-rich sequences were found at the centromeres of all chromosome pairs and the GC-rich sequences were found in paracentromeric location on over half of the chromosomal pairs. Different patterns of hybridization were found for the two subgroups of the AT-rich family. One type hybridized primarily to centromeres of one half to two thirds of the chromosomal pairs and the other type to centromeres of about three fourths of the chromosomal pairs. The pattern of hybridization with the GC-rich sequences varied somewhat between the cell lines consistent with interindividual variation in the location of paracentromeric heterochromatin. The 5S rRNA genes are found on the long arm of chromosome 3. Most of this chromosome arm is late replicating, but apparently does not contain either the AT-rich or GC-rich repetitive sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009271017998
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