ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
: Hydrostatic pressures (50 to 300 MPa) were applied at 0 °C for 50 min to determine the aggregation and viscoelastic properties of tilapia (Orechromis niloticus) myosin fragments: subfragment-1 (S-1) and rod. With pressure lower than 150-MPa treatment, S-1 underwent intermolecular interaction to increase its elastic properties. After a 200-MPa treatment, S-1 unfolded, aggregated, and decreased its solubility. A turbid solution was obtained after pressurization over 200 MPa. Further, S-1 transformed to a more elastic gel with pressure increasing. At 200 MPa, S-1 denatured entirely with no change of enthalpy detected by differential scanning calorimetry (DSC). However, all properties of rod did not change during pressurization. This study reveals that S-1 contributes the initiation of gel formation in myosin, and the role of rod is not clear. Keywords: hydrostatic pressure, S-1, rod, denaturation
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2004.tb09907.x
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