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  • 1
    Electronic Resource
    Electronic Resource
    A novel in vitro effect of the mucosal protective agent sofalcone – inhibition of chemotactic motility in Helicobacter pylori (2000)
    Oxford, UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 14 (2000), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Motility of Helicobacter pylori is essential for colonization. H. pylori has been shown to exhibit chemotactic activity toward urea and sodium and bicarbonate ions, which are secreted from the gastric epithelia. The importance of urease activity for chemotactic motility of H. pylori in a viscous environment has also been shown. Consequently, application of drugs inhibiting chemotactic motility has been proposed as a strategy for H. pylori eradication. This inhibitory effect can be evaluated through assay of chemotaxis and swarming. Materials and methods: H. pylori CPY3401 and ATCC43504 were grown on brucella agar plates/broth supplemented with 3% horse serum under microaerobic conditions (N2, 85%; O2, 5%; CO2, 10%). For motility assay, H. pylori cells grown on brucella-serum agar were stabbed into motility agar containing 0.35% refined agar in brucella-serum broth and the swarming zone was measured. For the chemotaxis assay, cells were suspended in 10 m m potassium phosphate buffer, pH 7.0, with 3% polyvinyl- pyrrolidone and assayed as described previously. Bacterial swimming in the fluid environment was observed under dark-field microscopy. Results: Numbers of bacteria attracted toward 1 μm flurofamide were reduced with increasing con- centrations of sofalcone (0.2–222 μm). In addition, the size of the swarming zone was reduced in motility agar containing 22 and 222 μm sofalcone. On the other hand, 22 μm sofalcone did not inhibit bacterial growth on day 3. Bacterial swimming speed in brucella broth was slower in the presence of 22 and 222 μm sofalcone than in its absence. Conclusion: Sofalcone was found to inhibit chemotactic motility of H. pylori. This drug may be useful for inhibiting the bacterium's ability to colonize the human stomach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.2000.014s1230.x
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  • 2
    Electronic Resource
    Electronic Resource
    Positive endometrial cytology associated with primary gastric adenocarcinomas: clinical and cytopathologic findings in 16 patients (2000)
    Springer
    International journal of clinical oncology 5 (2000), S. 229-235 
    Details
    ISSN: 1437-7772
    Keywords: Key words Endometrial cytology ; Gastric cancer ; Metastasis to the uterus ; Extra-uterine neoplasms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. Routine endometrial cytology occasionally includes malignant cells of extra-genital origin. We carried out this study to examine both the clinical and the cytopathologic characteristics of patients with positive endometrial cytology associated with gastric cancer. Methods. Sixteen patients whose endometrial smears contained malignant cells derived from gastric adenocarcinoma were studied. The patients' clinical symptoms, clinical courses, and cytologic and pathological findings were analyzed. Results. Among the 16 patients, 5 showed positive endometrial cytology prior to the initial treatment of gastric cancer, but after its diagnosis; 8 patients were being examined for relapse of gastric cancer; and, in the remaining 3 patients, the endometrial cytology led to the diagnosis of gastric cancer. Eleven patients had some clinical signs or symptoms, including abnormal vaginal discharge. Two patients were positive for serum carcinoembryonic antigen (CEA), while carbohydrate antigen (CA)125 was positive in 5 patients. The histological types of the primary gastric lesions were poorly differentiated adenocarcinoma (including signet ring cell adenocarcinoma) in 14 patients, and moderately differentiated tubular adenocarcinoma in 2 patients. Signet ring cells were observed on endometrial cytology in 12 patients. Four patients showed tumor diathesis on the smears. Metastatic lesions in the uterus were histologically confirmed in 5 patients. All 16 patients died as a result of peritoneal carcinomatosis within 12 months of the positive endometrial smears. Conclusion. Examination of routine reproductive tract smears, especially endometrial cytology, presents gynecologists with the opportunity to identify patients with gastric adenocarcinoma cells in the uterus and/or peritoneal cavity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012042
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