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  • 1
    ISSN: 1432-1831
    Keywords: Key words Differential display ; Gene expression ; Virus reactivation ; Epstein-Barr virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Stimulation of Epstein-Barr virus (EBV) genome-positive Burkitt lymphoma cells with the ganglioside IV3NeuAc-nLcOse4Cer leads to the induction of cell differentiation processes and activates the EBV lytic viral cycle. In cells of the Burkitt lymphoma line Raji differential expression of host cell genes was analysed in the early phase (150 min) post stimulation with the ganglioside to display the cell activities that precede the activation of the EBV lytic cycle using the differential display reverse transcription-polymerase chain reaction technique. Multiple fragment cDNAs derived from control cells and ganglioside-stimulated cells were amplified using random primers and displayed via polyacrylamide gel electrophoresis. The expression pattern of 8,400 bands was analysed. Eleven differentially expressed fragment cDNAs were reamplified and identified by nucleotide sequencing. Six of these could be identified as coding for proteins that may take part in virus reactivation and differentiation. The most striking finding was the induction of s-adenosylhomocysteine hydrolase (AHCY) expression. The cellular enzyme AHCY plays an important role in transmethylation reactions controlling the replication of several viruses. Thus, an involvement in EBV replication can be suggested.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Advances in computational mathematics 12 (2000), S. 273-288 
    ISSN: 1572-9044
    Keywords: multivariate polynomial interpolation ; sparse grids ; least solution ; universal method ; tractability ; 41A05 ; 41A63 ; 65D05 ; 41A25
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We study polynomial interpolation on a d-dimensional cube, where d is large. We suggest to use the least solution at sparse grids with the extrema of the Chebyshev polynomials. The polynomial exactness of this method is almost optimal. Our error bounds show that the method is universal, i.e., almost optimal for many different function spaces. We report on numerical experiments for d = 10 using up to 652 065 interpolation points.
    Type of Medium: Electronic Resource
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