ISSN:
1420-908X
Keywords:
Key words: Programmed cell death - Reactive oxygen species - Adhesion - Cytoskeleton - Tyrosine phosphorylation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract: Objective and design: To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following β 2-integrin activation, and further investigate the signal transduction pathways involved.¶Material: Human polymorphonuclear neutrophils.¶Treatment: Neutrophils were challenged with pansorbins coated with antibodies towards the β 2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 μg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 μM).¶Methods: Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.¶Results: Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p〈0.01), and activate the production of reactive oxygen species (p〈0.01). Pre-treatment with the inhibitors have no effect on anti-CD18 induced apoptosis.¶Conclusion: Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.¶
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s000110050616
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