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  • 2000-2004  (3)
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  • 1
    ISSN: 1440-1797
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background and Aim:  Tubular atrophy is a major feature of most renal diseases and is closely associated with the loss of renal function. The present study sought to investigate whether Fas/FasL-induced tubular epithelial cell apoptosis was a feature of experimental diabetic nephropathy. The effects of renoprotective therapy with blockade of the renin-angiotensin (RAS) system were also examined.Method:  Six-week-old female Ren-2 rats were injected with streptozotocin and maintained diabetic for 12 weeks. Further groups of diabetic rats were treated with the angiotensin-converting enzyme inhibitor, perindopril, for 12 weeks.Results:  Widespread apoptosis, identified by using mediated Terminal dUTP nick-end labelling (TUNEL) staining was noted in the tubules of diabetic Ren-2 rats. These changes were associated with an increase in both Fas mRNA and Fas L (ligand) within the tubules (P 〈 0.01). Treatment of diabetic Ren-2 rats with perindopril (6 mg/kg per day) reduced the apoptosis to control levels and was associated with a reduction in Fas mRNA and Fas L protein (P 〈 0.05).Conclusion:  In conclusion, Fas/Fas L-induced tubular apoptosis is a feature of diabetic Ren-2 rats and is attenuated by the blockade of the RAS.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to provide evidence for elucidating the mechanisms of neurokinin-3 receptor (NK3) in spinal pain modulation. First, colocalization of NK3 with the µ-opioid receptor (MOR1) was studied in the spinal dorsal horn of the rat. Confocal microscopy showed that about 44% of NK3-expressing neurons in laminae I and II were immunoreactive for MOR1, which corresponded to about 93% of the total population of MOR1-containing neurons in these laminae. Second, the relationship between NK3/MOR1-coexpressing neurons and those that express nitric oxide synthase (NOS) was examined by using a triple immunofluorescent staining method. About 37% of NK3-immunoreactive neurons were also NOS-immunoreactive, which constituted about 82% of NOS-immunoreacitve neurons in the superficial laminae. However, no triple-labelled neurons were detected. The present results indicate that there are two major distinct subpopulations of NK3-expressing neurons in the superficial dorsal horn, which suggests that the involvement of NK3 receptor in spinal nociception could be mediated by two distinct mechanisms, i.e. opioid and nitric oxide.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 274 (2000), S. 557-579 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Pure luminosity evolution models for galaxies provide an unacceptable fit to the redshifts and colors of faint galaxies. In this paper we demonstrate, using HST morphological number counts derived both from the I 814-band of WFPC2 in the Medium Deep Survey (MDS) and the Hubble Deep Field (HDF) and from the H 1.6-band of NICMOS, and ground-based spectroscopic data of the Hawaii Deep Field and the Canada-France Redshift Survey, that number evolution is necessary for galaxies, regardless of whether the cosmic geometry is flat, open, or Λ-dominated. Furthermore, we show that the number evolution is small at redshifts of z〈1, but large at z〉1, and that this conclusion is valid for all the three cosmological models under consideration. If the universe is open or Λ-dominated, the models, which are subject to the constraint of the conservation of the comoving mass density of galaxies, naturally predict a population of star-forming galaxies with the redshift distribution peaking at z=2∼ 3, which seems to be consistent with the recent findings from Lyman-break photometric selection techniques. If the cosmological model is flat, however, the conservation of the comoving mass density is invalid. Hence, in order to account for the steep slope of B-band number counts at faint magnitudes in the flat universe, such a star-forming galaxy population has to be introduced ad hoc into the modelling alongside the merger assumption.
    Type of Medium: Electronic Resource
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