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  • 1
    Digitale Medien
    Digitale Medien
    An EMS-induced wheat mutant restoring fertility against photoperiod-sensitive cytoplasmic male sterility (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Triticum aestivum cv. ‘Norin 26’ with Aegilops crassa cytoplasm shows photoperiod-sensitive cytoplasmic male sterility (PCMS). This alloplasmic line is almost completely male-sterile under long-day conditions (≥ 15h), but highly male-fertile under short-day conditions (≤ 14.5h). To obtain male—fertile mutants against PCMS, seeds of the alloplasmic line were treated with ethyl methane sulfonate (EMS). The M3 generation was evaluated for PCMS expression, and one fertility-restoring (FR-mutant) line showing high male fertility under the long-day conditions was selected. Reciprocal F, hybrids between the FR-mutant and the alloplasmic ‘Norin 26’ showed male sterility under the long-day conditions, and continuous segregation with respect to the degree of fertility restoration occurred in their F2 generations. These results indicate that multiple recessive mutations with minor effects, induced in the nuclear genome, are involved in the fertility restoration. In fact, no restriction-fragment-length polymorphisms of mitochondrial DNA between the FR-mutant and the alloplasmic ‘Norin 26’ are found.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00794.x
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  • 2
    Digitale Medien
    Digitale Medien
    Deficiency of cox1 gene expression in wheat plants with Aegilops columnaris cytoplasm (1996)
    Springer
    Current genetics 30 (1996), S. 509-514 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Key words Mitochondria ; cox genes ; Transcription ; Alloplasmic wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Wheat plants with Aegilops columnaris cytoplasm are characterized by growth inhibition and partial male sterility and show an impaired mitochondrial cytochrome c oxidase activity. We designed this study to clarify the functional relationship between this impaired cytochrome c oxidase activity and possible structural alterations to the mitochondrial genes cox1, cox2 and cox3 that encode three subunits of the cytochrome c oxidase. Using restriction mapping and DNA sequencing, we found major rearrangements in the flanking regions of the cox1 and cox3 genes. In Northern-blot analysis, we detected two different cox3 transcripts and a reduced level of a cox2 transcript, whereas we could not detect a transcript of cox1. These results suggest that rearrangements of the flanking regions of the cox1 gene may result in the severe suppression of its transcription, and subsequently, may lead to the impaired cytochrome c oxidase activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002940050163
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  • 3
    Digitale Medien
    Digitale Medien
    Wheat phylogeny determined by RFLP analysis of nuclear DNA. 3. Intra- and interspecific variations of five Aegilops Sitopsis species (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 928-934 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Intraspecific variation ; Interspecific variation ; Aegilops Sitopsis species ; RFLP analysis ; Nuclear DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The level of intra- and interspecific variations on nuclear DNA in five Aegilops species of the Sitopsis section were investigated using restriction fragment length polymorphism (RFLP) analysis. A total of 18 accessions, i.e. 7 of Ae. speltoides, 3 of Ae. longissima, 2 of Ae. searsii, 3 of Ae. sharonensis and 3 of Ae. bicornis, were used. One accession each of Triticum aestivum, T. durum, T. urartu and Ae. squarrosa was included as reference material. Five enzymes and 20 probes were used. Among the five Sitopsis species studied, Ae. speltoides had the largest intraspecific variation which was as high as the interspecific variation observed among the other four species. The section Sitopsis was divided into two distinct groups: one containing only Ae. speltoides and the other, Ae. longissima, Ae. searsii, Ae. sharonensis and Ae. bicornis. This grouping by RFLP analysis is in agreement with the taxonomical classification of the subsections.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050213
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  • 4
    Digitale Medien
    Digitale Medien
    Wheat phylogeny determined by RFLP analysis of nuclear DNA. 3. Intra- and interspecific variations of five Aegilops Sitopsis species (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 928-934 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Intraspecific variation ; Interspecific ; variation ; Aegilops Sitopsis species ; RFLPanalysis ; Nuclear DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The level of intra- and interspecific variations on nuclear DNA in five Aegilops species of the Sitopsis section were investigated using restriction fragment length polymorphism (RFLP) analysis. A total of 18 accessions, i.e. 7 of Ae. speltoides, 3 of Ae. longissima, 2 of Ae. searsii, 3 of Ae. sharonensis and 3 of Ae. bicornis, were used. One accession each of Triticum aestivum, T. durum, T. urartu and Ae. squarrosa was included as reference material. Five enzymes and 20 probes were used. Among the five Sitopsis species studied, Ae. speltoides had the largest intraspecific variation (π=0.061), which was as high as the interspecific variation observed among the other four species. The section Sitopsis was divided into two distinct groups: one containing only Ae. speltoides and the other, Ae. longissima, Ae. searsii, Ae. sharonensis and Ae. bicornis. This grouping by RFLP analysis is in agreement with the taxonomical classification of the subsections.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224032
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  • 5
    Digitale Medien
    Digitale Medien
    Wheat phylogeny determined by RFLP analysis of nuclear DNA. 2. Wild tetraploid wheats (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 129-134 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; RFLP ; Triticum dicoccoides ; T. araraticum ; Genetic divergence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Intra- and inter-specific variations in the nuclear DNA of Triticum dicoccoides Körn. (2n = 28, genome constitution AABB) and T. araraticum Jakubz. (2n = 28, AAGG), wild species, respectively, of the Emmer and Timopheevi group, were studied by restriction fragment length polymorphism (RFLP) analysis. Total DNAs of 32 T. dicoccoides and 24 T. araraticum accessions, collected from throughout the distribution areas of these species, were treated with two 6-bp cutters and hybridized with 30 nuclear DNA clones as probes to detect RFLPs. A total of 167 hybrid bands were observed per accession. All the enzyme-probe combinations showed RFLPs between accessions. The average genetic distance between the T. dicoccoides accessions was 0.0135 ± 0.0031 and that between the T. araraticum accessions 0.0036 ± 0.0015, indicative of about a four-fold intraspecific variation in T. dicoccoides as compared to T. araraticum in terms of genetic distance. No significant genetic differentiation was found for the geographical populations of these species, the genetic distance between the two species being 0.0482 ± 0.0022. The interspecific divergence corrected for intraspecific divergence was 0.0395, about three times that for T. dicoccoides and 11 times that for T. araraticum. The results show that in the wild state the Emmer and Timopheevi groups are clearly differentiated and that T. dicoccoides has much greater variation than T. araraticum, suggesting a relatively recent origin for the latter and therefore a diphyletic origin for these species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221006
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