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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Secretoneurin (SN) is a neuropeptide derived from secretogranin II that is found in brain and endocrine tissues. The aim of the present study was to determine the influence of this novel peptide on dopamine (DA) release from rat striatum using the microdialysis technique. Rat SN (1–30 µmol/L added to the dialysis buffer) enhanced DA outflow of awake rats in a concentration-dependent way without marked effects on the outflow of 3,4-dihydroxyphenylacetic acid or homovanillic acid. The increase in extracellular DA content caused by the peptide was observed throughout the entire period of administration (up to 4 h). Human SN and its 15-amino-acid C-terminal sequence also increased DA outflow, but the effects were smaller than those of rat SN. Two other peptides derived from secretogranin II were without effect on DA efflux. These results establish that SN has a pronounced effect on DA release under in vivo conditions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    European journal of neuroscience 8 (1996), S. 0 
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An antiserum was raised against the peptide PE-11 whose sequence is present in the chromogranin B molecule. The antiserum reacts only with the free C-terminal end of this peptide. PE-11 immunoreactivity in brain was characterized by molecular size exclusion high performance liquid chromatography. Only the free peptide and a N-terminally elongated peptide were detected, indicating that proteolytic processing of chromogranin B in brain is quite extensive. In immunohistochemistry PE-11 immunoreactivity was found in varicosities, fibres and perikarya throughout the brain. Strong staining was detected in the shell sector of the nucleus accumbens, in the lateral septum, in subregions of the extended amygdala, in some areas of the hippocampus and of the hypothalamus, in the locus coeruleus, in the Purkinje cells of the cerebellum and in the dorsal horn of the spinal cord. Our results, which demonstrate significant processing of chromogranin B in brain and its widespread distribution, can be taken as an indication that chromogranin B represents a precursor of peptides with functional relevance for this organ.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The cytokine profile of CD4+, CD8+ T cells, γδ+ T cells and natural killer (NK) cells (CD94+CD3–) was studied in a patient with visceral leishmaniasis (VL). The otherwise healthy, human immunodeficiency virus-negative patient acquired the disease in Tuscany, Italy. Diagnosis was made by demonstration of high concentrations of antibodies against Leishmania antigens in serum. Flow cytometry for the detection of intracellular interferon-γ (IFN-γ), interleukin (IL)-2, IL-4, IL-6, IL-10, IL-13 and tumour necrosis factor (TNF)-α expression in peripheral blood mononuclear cells stimulated with phorbol 12-myristate 13-acetate and ionomycin was performed, followed by treatment with liposomal amphotericin B. CD4+ cells were identified as major cytokine-expressing cells, capable of producing both type 1 and type 2 cytokines. A high frequency of IL-4- and IL-13-expressing CD8+ cells was noted. NK cells and γδ+ T cells, thought to be involved in innate host defences against Leishmania, expressed IFN-γ and TNF-α. Ten per cent of γδ+ T cells expressed IL-10, predominantly together with IFN-γ, suggesting additional immune-regulatory roles for this T-cell subset in VL.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Secretoneurin is a recently described peptide derived by endoproteolytic processing from secretogranin II, previously named chromogranin C. In this study, we have investigated the distribution of secretoneurin-like immunoreactivity in the human hippocampus in controls and in Alzheimer's disease patients, and compared the staining pattern to that of calretinin. Secretoneurin-like immunoreactivity is present throughout the hippocampal formation. At the border of the dentate molecular layer and the granule cell layer, a band of dense secretoneurin immunostaining appeared. In this part, as in the area of the CA2 sector, the high density of secretoneurin-immunoreactivity coincided with calretinin-like immunoreactivity. The mossy fibre system displayed a moderate density of secretoneurin-immunoreactivity. In the entorhinal cortex, a particularly high density of secretoneurin-immunoreactivity was observed. The density of secretoneurin-like immunoreactivity was significantly reduced in the innermost part of the molecular layer and in the outer molecular layer of the dentate gyrus in Alzheimer's disease. For calretinin-like immunoreactivity, a less pronounced decrease was found in the innermost part of the molecular layer. About 40–60% of neuritic plaques were secretoneurin-immunopositive.This study shows that secretoneurin is distinctly distributed in the human hippocampus and that significant changes of secretoneurin-like immunoreactivity occur in Alzheimer's disease, reflecting synaptic loss.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ecology of freshwater fish 4 (1995), S. 0 
    ISSN: 1600-0633
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract– The mechanisms leading to spatial segregation along optical habitat dimensions in closely related, co-occurring fishes were investigated. In two experimental series, six laboratory-reared cyprinid species were faced to six novel optical patterns. These were positioned either laterally, at the bottom, or at the water surface, in one of three compartments of a large tank. When a bottom pattern was presented, the primarily bottom-dwelling species Vimba elonguta and Abranzis brama chose the test compartment at high frequencies, indicating a significant preference. More surface-oriented species like Chalcalburnus chalcoides mento and Scardinius erythrophthalmus developed similar preferences in response to surface patterns. V. elongata, A. brama, and Rutilus rutilus showed significant preferences for a photograph of submerged plants. Also, V. elongata developed preferences for several optical patterns. These data confirm earlier studies on V. elongata habitat selection and foraging behaviour which suggest a highly exploratory lifestyle. S. erythrophthalmus and Leuciscus cephalus showed significant avoidances for laterally positioned optical patterns associated with reduced interindividual distances, indicating marked neophobic responses. The establishment of species-specific preferences or avoidances during the two experimental series was influenced by learning processes.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature medicine 1 (1995), S. 869-873 
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Pioneers in xenotransplantation (transplantation of organs from one species to another) abandoned their efforts decades ago largely because of the generally poor results obtained and because of the emergence of clinical allotransplantation. Currently, interest in xenotransplantation research is ...
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für angewandte Mathematik und Physik 10 (1959), S. 438-441 
    ISSN: 1420-9039
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Integral equations and operator theory 38 (2000), S. 222-250 
    ISSN: 1420-8989
    Keywords: Primary 34A55 ; 47E05 ; Secondary 34B20 ; 34L05 ; 47B25
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We consider a singular two-dimensional canonical systemJy′=−zHy on [0, ∞) such that at ∞ Weyl's limit point case holds. HereH is a measurable, real and nonnegative definite matrix function, called Hamiltonian. From results of L. de Branges it follows that the correspondence between canonical systems and their Titchmarsh-Weyl coefficients is a bijection between the class of all Hamiltonians with trH=1 and the class of Nevanlinna functions. In this note we show how the HamiltonianH of a canonical system changes if its Titchmarsh-Weyl coefficient or the corresponding spectral measure undergoes certain small perturbations. This generalizes results of H. Dym and N. Kravitsky for so-called vibrating strings, in particular a generalization of a construction principle of I.M. Gelfand and B.M. Levitan can be shown.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Integral equations and operator theory 22 (1995), S. 360-374 
    ISSN: 1420-8989
    Keywords: 34 A 55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 18 (1996), S. 353-357 
    ISSN: 0392-6737
    Keywords: Mössbauer spectra ; Spectra ; photodissociation and photoionization of biomolecules ; bioluminescence ; Measurement of rate constants ; reaction cross-sections and activation energy ; Conference proceedings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Partial photodissociation of two carbonmonoxy-porphyrin complexes, a bridged and a fenced one, has been achieved. The results from monitoring the recombination point to an important influence of the solvent matrix on the recombination rates.
    Type of Medium: Electronic Resource
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