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  • 1
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We have used polarized neutron reflection to determine the layer-dependent spin orientations in an antiferromagnetically coupled 100 A(ring) Cr/50 A(ring) Fe/15 A(ring) Cr/50 A(ring) Fe/Si sandwich structure prepared by sputtering. At low field, the net Fe layer magnetic moments align in an asymmetric canted orientation with a near zero total magnetic moment for the sample. At high fields, a canted state, nearly symmetric with respect to the applied field direction is observed and the magnetization in each layer does not reach the bulk saturation value until the layers are ferromagnetically aligned. The behavior is discussed in the context of current theories of exchange coupling. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 97 (1998), S. 345-355 
    ISSN: 1432-2242
    Keywords: Key words Candidate genes ; Disease resistance genes ; Germplasm diversity ; Host-pathogen interaction ; Molecular marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genes cloned from diverse plants for resistance to different pathogens have sequence similarities in domains presumably involved in pathogen recognition and signal transduction in triggering the defense response. Primers based on the conserved regions of resistance genes often amplify multiple fragments that may not be separable in an agarose gel. We used denaturing polyacrylamide-gel electrophoresis to detect PCR products of plant genomic DNA amplified with primers based on conserved regions of resistance genes. Depending upon the primer pairs used, 30–130 bands were detected in wheat, rice, and barley. As high as 47%, 40%, and 27% of the polymorphic bands were detected in rice, barley, and wheat, respectively, and as high as 12.5% of the polymorphic bands were detected by certain primers in progeny from a cross of the wheat cultivars ‘Stephens’ and ‘Michigan Amber’. Using F6 recombinant inbred lines from the ‘Stephens’בMichigan Amber’ cross, we demonstrated that polymorphic bands amplified with primers based on leucine-rich repeats, nucleotide-binding sites and protein kinase genes, were inherited as single loci. Linkages between molecular markers and stripe rust resistance genes were detected. This technique provides a new way to develop molecular markers for assessing the genetic diversity of germplasm based upon potential candidate resistance genes in diverse species.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Disease resistance ; Oryza species ; Interspecific hybridization ; Xanthomonas oryzae pv. oryzae ; Pyricularia grisea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Oryza minuta J. S. Presl ex C. B. Presl is a tetraploid wild rice with resistance to several insects and diseases, including blast (caused by Pyricularia grisea) and bacterial blight (caused by Xanthomonas oryzae pv. oryzae). To transfer resistance from the wild species into the genome of cultivated rice (Oryza sativa L.), backcross progeny (BC1, BC2, and BC3) were produced from interspecific hybrids of O. sativa cv ‘IR31917-45-3-2’ (2n=24, AA genome) and O. minuta Acc. 101141 (2n=48, BBCC genomes) by backcrossing to the O. sativa parent followed by embryo rescue. The chromosome numbers ranged from 44 to 47 in the BC1 progeny and from 24 to 37 in the BC2 progeny. All F1 hybrids were resistant to both blast and bacterial blight. One BC1 plant was moderately susceptible to blast while the rest were resistant. Thirteen of the 16 BC2 progeny tested were resistant to blast; 1 blast-resistant BC2, plant 75-1, had 24 chromosomes. A 3 resistant: 1 susceptible segregation ratio, consistent with the action of a major, dominant gene, was observed in the BC2F2 and BC2F3 generations. Five of the BC1 plants tested were resistant to bacterial blight. Ten of the 21 BC2 progeny tested were resistant to Philippine races 2, 3, and 6 of the bacterial blight pathogen. One resistant BC2, plant 78-1, had 24 chromosomes. The segregation of reactions of the BC2F2, BC2F3, and BC2F4 progenies of plant 78-1 suggested that the same or closely linked gene(s) conferred resistance to races 2, 3, 5, and 6 of the bacterial blight pathogen from the Philippines.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2242
    Keywords: Rice blast ; RFLP ; Retrotransposon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic map of Magnaporthe grisea (anamorph=Pyricularia oryzae and P. grisea), the causal agent of rice blast disease, was generated from segregation data utilizing 97 RFLP markers, two isoenzyme loci and the mating type locus among progeny of a cross between parental strains Guy 11 and 2539. Of the seven chromosomes of M. Grisea, three were resolved by contour-clamped homogeneous electric field (CHEF) electrophoresis, while the remaining four migrated as two doublet bands. By utilizing differences between CHEF mobilities of unresolved chromosomes from the parental strains, Southern analysis with selected markers allowed the chromosomal assignment of all linkage groups. A small translocation involving 1 marker was found in the parental strains used to produce the segregating population from which the map was constructed. Nine classes of repetitive DNA elements were found in the genome of a fungal isolate pathogenic to rice. These occurred only a few times or not at all in the genomes of isolates showing reduced virulence on rice. One repetitive DNA was shown to have structural similarity to the Alu sequences found in primates, a sequence similarity to the copia-like elements of Drosophila, and peptide similarity to transposable elements found in Drosophila, other fungi, and higher plants.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Circuits, systems and signal processing 18 (1999), S. 59-73 
    ISSN: 1531-5878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Chaotic systems provide a simple means of generating deterministic signals that resemble white noise. It is this noise-like property that provides the potential for applying chaotic systems in communications. In this work, we report a detailed study of the logistic map for use as direct-sequence spread-spectrum (DS/SS) codes. The advantages of the chaotic DS/SS codes are the almost unlimited number of distinct sequences of arbitrary lengths, the ease of generating these sequences, and the increased privacy afforded by the noise-like appearance of these sequences. Some design criteria are provided from the correlation properties of these sequences, and bit-error rate (BER) results are generated by Monte Carlo simulations.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 15 (1996), S. 1833-1835 
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-4943
    Keywords: Ribosome-inactivating proteins ; luffin-α ; 3D structure ; neural network ; substrate interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A fundamental problem in biochemistry and molecular biology is understanding the spatial structure of macromolecules and then analyzing their functions. In this study, the three-dimensional structure of a ribosome-inactivating protein luffin-α was predicted using a neural network method and molecular dynamics simulation. A feedforward neural network with the backpropagation learning algorithm were trained on model class of homologous proteins including trichosanthin andα-momorcharin. The distance constraints for the Cα atoms in the protein backbone were utilized to generate a folded crude conformation of luffin-α by model building and the steepest descent minimization approach. The crude conformation was refined by molecular dynamics techniques and a simulated annealing procedure. The interaction between luffin-α and its analogous substrate GAGA was also simulated to understand its action mechanism.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The availability of high quality SmBa2Cu3O6.5 seeds of known orientation is essential for the fabrication of a large grain YBa2Cu3O7−δ (YBCO) superconductor via a seeded peritectic solidification technique. The suitable seed must have a close lattice parameter match to YBCO and a relatively high melting temperature. We report a melt texturing process for the fabrication of SmBa2Cu3O7−δ (Sm-123) seeds using SrTiO3 to control grain nucleation. The physical and structural properties of the seeds were confirmed by differential thermal analysis (DTA), X-ray diffraction (XRD) and scanning electron microscopy (SEM). Seeds prepared in this study were used to grow large single grain YBCO superconductors of up to 2 cm in diameter.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 33 (1998), S. 1083-1089 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract High values of critical current density, Jc, in large-grain superconducting melt-processed YBa2Cu3O7-δ (YBCO, Y-123) have been reported to correlate closely with the distribution of Y2BaCuO5 (Y-211) particles in the Y-123 phase matrix. Extensive image analysis of the homogeneity of the Y-211 particle distribution in a large-grain sample has been performed on high-resolution, secondary scanning electron micrographs of seeded melt-processed YBCO. The variation of key parameters, such as area fraction, number density and size along the a/b axis of the Y-123 grain has been investigated in detail as a function of distance from the centre of the seed. Both area fraction and number density of Y-211 particles are observed to increase continuously with distance, whereas the mean and standard deviation of the size distribution decrease slightly towards the grain boundary. This suggests that the increase in area fraction can be attributed to an increase in number density of the Y-211 particle distribution along the a/b axis of the specimen. The implications for Y-211 particle pushing, ripening and coalescence are discussed in the light of these results. © 1998 Chapman & Hall
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 244-248 
    ISSN: 1434-4726
    Keywords: Irradiation ; Middle ear mucosa ; Cilia Effusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Adult Sprague-Dawley rats (n = 64) were used in this study to replicate in an animal model tissue change formed clinically during radiotherapy for the treatment of head and neck malignancy. Gamma irradiation was administered to both ears of each rat, using a cobalt-60 machine. A total dose of 30 Gy was administered, with 500 cGy/fraction given on 6 consecutive days to a time-dose fractionation value of 81. Animals were then sacrificed at sequential intervals. The tympanic bullae were removed and the mucosa was processed for scanning electron microscopy. Tissue findings indicated that irradiation might initially have no influence on the clearance activity of the middle ear. However, ultrastructural changes showed stagnation of secretion between cilia, falling off and different directions of the cilia, fused or collapsed cilia, and eustachian tube dysfunction (with mucosal edema). These findings possibly account for the occurrence of middle ear effusions in patients during and after radiotherapy for head and neck malignancy. It is our concept that insertion of a ventilation tube is necessary if abnormal mucociliary function appears, in order to clear and prevent the accumulation of any middle ear effusions and prevent further mucociliary dysfunction.
    Type of Medium: Electronic Resource
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