ISSN:
1612-1112
Schlagwort(e):
Gas chromatography-mass spectrometry
;
Column liquid chromatography
;
Supercritical fluid extraction
;
Bile acids
;
Bovine bile hydrolysates
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary The use of supercritical fluid extraction (SFE) as an alternative to conventional liquid extraction has been investigated to conventional liquid extraction has been investigated for the isolation of bile acids (cholic acid, deoxycholic acid and chenodeoxycholic acid) from the solid material obtained by bovine bile hydrolysis. Initial studies indicated that bile acids are not soluble in supercritical CO2, consequently the addition of the polar modifier methanol to the CO2 extracting fluid was evaluated. Quantitative recoveries (〉88.5%) were obtained for the bile acids in 20 min with 15% (v/v) methanol in CO2 at 220 atm and 70°C. A comparison of SFE with pyridine extraction was performed on samples of hydrolyzed crude bovine bile. The SFE method yielded recoveries higher than 82.7% compared with conventional liquid extraction. Moreover, SFE minimized sample handling, reduced the consumption of harmful solvents and provided conditions suitable for selective extractions of bile acids from bovine bile raw materials.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02467648
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