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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Der Unfallchirurg 100 (1997), S. 39-43 
    ISSN: 1433-044X
    Keywords: Schlüsselwörter Implantatwerkstoffe ; Hydroxylapatit ; Titan ; Stahl ; Biokompatibilität ; Fibroblasten-Zellinie (3T3) ; Osteoblasten-Zellinie (MC3T3-E1) ; humane Osteoblasten ; Zellproliferation (MTT) ; Alkalische Phosphatase (ALP) ; Key words Biomaterials ; Hydroxyapatite ; Titanium ; Steel ; Biocompatibility ; Fibroblast cell line (3T3) ; ``Osteoblast-like'' cell line (MC3T3-E1) ; Human osteoblasts ; Cellproliferation (MTT) ; Alkaline phosphatase activity (ALP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Two different types of cell-line and fresh human osteoblasts, cultured from cancellous bone grafts from the iliac crest, were used for the study. Three different biomaterials were compared regarding biocompatibility: titanium, steel and hydroxyapatite. The cells were fibroblast cell line (3T3), ``osteoblast-like'' cell line (MC3T3-E1), and fresh human osteoblasts (HOB) which we cultured in our laboratory. 5×104 cells of each type were seeded on the three different bone implants. All experiments were performed in triplicate. Cell proliferation and alkaline phosphatase activity were determined 24 and 72 h after the cells were plated on the biomaterials. Human osteoblast growth was better on titanium than on steel and hydroxyapatite. The most remarkable observation was the continuously decreasing alkaline phosphatase activity (ALP) of ``osteoblast-like'' cells (MC3T3-E1) and human osteoblasts (HOB) on hydroxyapatite. In conclusion, our in vitro observations suggest that the best cell/material interactions were with human osteoblasts (HOB) and titanium.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1084
    Keywords: Key words: Stereolithography ; Rapid prototyping ; Computed tomography ; Calcaneal fracture ; ROC curves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The purpose of this study was to evaluate and compare the diagnostic performance of stereolithography vs workstation-based three-dimensional (3D) reformations in intra-articular calcaneal fractures. A total of 30 intra-articular calcaneal fractures were examined using standard radiographs, coronal CT scans, and 2D and 3D reformations. The CT data were transferred to an outside institution, and stereolithograms were produced from photopolymer resin employing a laser beam system. 3D reformations and stereolithograms were analyzed in a blinded fashion by two staff radiologists. Receiver-operating-characteristic (ROC) curves were obtained for six clinically significant fracture components. Standard radiographs, coronal CT scans, and 2D reformations served as the standard of reference. The area under the ROC curves for 3D reformations and stereolithograms were 1.0 and 0.98 for abnormal tuber angles, 0.91 and 0.91 for anterior and middle talo-calcaneal joint involvement, 0.90 and 0.95 for involvement of the posterior talo-calcaneal joint, 0.65 and 0.78 for the presence of a lateral bulge, 0.80 and 0.81 for the involvement of the calcaneocuboidal joint, and 0.62 and 0.67 for the presence of a “tongue-type” fracture. No statistically significant difference was demonstrated for the two methods (Wilcoxon signed-rank test, p = 0.138). Based on our results stereolithograms did not prove to be statistically superior to workstation-based 3D reformations. Stereolithograms may still be useful for teaching purposes and for surgical planning at a thinking-efficacy level.
    Type of Medium: Electronic Resource
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