ISSN:
1075-4261
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Physics
Notes:
Measurements of optical characteristics such as light scattering and fluorescence of components in bacterial cells have been used to sort cells and to identify different classes of bacteria in a mixed suspension. More detailed studies require a knowledge of the optical properties of individual components of the cells. Because cells are composed largely of proteins, a measurement of the optical constants of horseradish peroxidase, a globular protein, would permit modeling of the refractive index profiles of complex inhomogeneous structures such as a bacterial spore. Spectral reflectance and transmittance measurements combined with Kramers-Kronig analyses have been used to obtain the real (n) and imaginary (k) parts of the complex refractive index N = n + ik of horseradish peroxidase over the wavelength interval from 0.13 to 2.5 μm. Samples were in the form of thin solid films, pressed pellets, and solutions in water. For wavelengths less than 0.6 μm, good agreement was obtained between the optical constants of the material derived from measurements made on the solid films and on the solutions in water. © 1997 John Wiley & Sons, Inc. Biospect 3: 73-80, 1997
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
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