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  • 1995-1999  (9)
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  • 1
    Electronic Resource
    Electronic Resource
    Anatomical and ultrastructural studies of chemical defence in the sponge Dysidea fragilis (1998)
    Springer
    Marine biology 131 (1998), S. 639-645 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The marine sponge Dysidea fragilis from El Mar Menor, a hypersaline coastal lagoon (Murcia, Spain), contains the furanosesquiterpenoid ent-furodysinin as the major secondary metabolite. D. fragilis emits a defensive white fluid when it is disturbed. Electron micrographs of this fluid revealed intact vesiculated cells together with other amorphous material. Dissociated cells are more rounded in shape but maintain the same ultrastructural features as cells observed in ultra-thin sections of the whole sponge. The defensive secretion is composed mainly of sponge cells with abundant light vesicles. Sometimes these light vesicles appear to open into the intercellular space; this correlates with surface blebs on these cells observed under scanning electron microscopy. The intracellular location of ent-furodysinin was confirmed by Erlich staining. In laboratory assays, we examined the role of ent-furodysinin as a feeding deterrent to generalist fish predators. It was isolated from D. fragilis and incorporated into a carrageenan-based artificial diet. The addition of ent-furodysinin to the artificial diet reduced feeding by the fish Thalassoma pavo. Similarly, fish did not feed on artificial diet above which defensive secretion of D. fragilis had been ejected with a small syringe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050356
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification and characterization of an extracellular protease activity produced by the marine Vibriosp 60 (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 136 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The marine fish pathogen Vibrio sp. 60 has been used as a host for heterologous expression of the Escherichia coli heat-labile enterotoxin B-subunit and derivatives carrying a C-terminal extension. In this study, a chimeric enterotoxin B-subunit with an extension corresponding to the carboxy-terminal nine amino acids -Tyr-Ala-Gly-Ala-Val-Val-Asn-Asp-Leu-cooH from the small subunit of herpes simplex virus type 1-encoded ribonucleotide reductase, is shown to be proteolytically cleaved in the extracellular medium by a single protease that is secreted by the host strain. Such protease behaves as a typical metalloprotease, being inhibited by EDTA but not by a serine protease inhibitor. Purification and amino acid composition analysis of the two proteolysis products revealed a specific cleavage of the peptide bond between amino acids glycine and alanine of the nine amino acid extension with loss of activity. The above observation is relevant for the biotechnological exploitation of Vibrio sp. 60.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08022.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Astrocytes and Microglia Produce Interleukin-12 p40 (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 795 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb52684.x
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  • 4
    Electronic Resource
    Electronic Resource
    Depolarized Rayleigh scattering in water up to supercritical conditions (1995)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 102 (1995), S. 6975-6981 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Depolarized Rayleigh spectra of water have been measured as a function of density along several isotherms in both liquid and supercritical phases. At all the thermodynamical states the spectral line shape can be fitted by a Lorentzian line superimposed to an exponential background. The behavior of the fitting parameters shows that at temperatures lower than ∼470 K the temperature is the most relevant parameter in determining the dynamics of the system, whereas above this temperature both temperature and density play a relevant role. Both the line shape and intensity of the spectra can be consistently interpreted in the framework of the first-order dipole induced dipole approximation to the collision-induced light scattering. The data show a clear evidence for cancellation effects. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.469090
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  • 5
    Electronic Resource
    Electronic Resource
    Light and neutron scattering studies of the OH stretching band in liquid and supercritical water (1998)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 108 (1998), S. 450-454 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: The hydrogen projected OH stretching density of states has been determined by an inelastic neutron scattering experiment in liquid and supercritical water. The results, compared with new measurements of the isotropic Raman spectra at the same state conditions, support the interpretation of the Raman spectra in terms of superposition of the allowed ν1 band with the overtone of the ν2 band. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.475407
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  • 6
    Electronic Resource
    Electronic Resource
    Cloning of the Human Puromycin-Sensitive Aminopeptidase and Evidence for Expression in Neurons (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 68 (1997), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The puromycin-sensitive aminopeptidase (PSA) is thought to contribute to the degradation of enkephalins. Besides being the most abundant aminopeptidase in the brain, PSA is expressed in other organs as well. From a human fetal brain cDNA library, we have isolated cDNA encoding the human PSA (huPSA) protein. The isolated cDNA gave rise to a protein with a molecular mass of 99 kDa. Compared with mouse PSA, homology at the amino acid and cDNA level was 98 and 93%, respectively. Translation of the huPSA was found to be initiated at the second of two possible start codons, as shown by studies with antibodies directed against peptide sequences of both potential N-terminal regions. Northern blot analysis with RNA isolated from different human organs demonstrated that the huPSA transcript is strongest but not exclusively expressed in the brain. Vesicular stomatitis virus epitope-tagged huPSA protein was expressed in HeLa cells and found to be localized in the cytoplasm, especially in the perinuclear region. By in situ hybridization, huPSA transcript could be identified in cortical and cerebellar neurons, whereas glial cells and blood vessels remained negative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1997.68030889.x
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The antidepressant rolipram suppresses cytokine production and prevents autoimmune encephalomyelitis (1995)
    [s.l.] : Nature Publishing Group
    Nature medicine 1 (1995), S. 244-248 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] In multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE) the cytokines tumour necrosis factor-α (TNF), lymphotoxin-α (LT), and interferon-gamma (IFN-γ) are of central pathogenetic importance. A therapy capable of stopping neurological ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm0395-244
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  • 8
    Electronic Resource
    Electronic Resource
    Implantation of the nail plate in plastic surgery (1997)
    Springer
    European journal of plastic surgery 20 (1997), S. 33-36 
    Details
    ISSN: 1435-0130
    Keywords: Nail plate ; Support ; Eyelids ; Ear
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A series of cases are presented to illustrate the possible uses of the nail plate as an alternative support in reconstruction of some free edges (nasal ala, eyelid, auricle of the ear), and laminar bone parts, such as the floor of the orbit. This technique was used experimentally by the authors many years ago and has subsequently been used in clinical practice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01366524
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Fructose syrups and ethanol production by selective fermentation of inulin (1995)
    Springer
    Current microbiology 30 (1995), S. 325-330 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Jerusalem artichoke is a favorable substrate for inulin or fructose syrup production. The sugar content and the fructose ratio of inulin depend on various factors, particularly on the date of harvest. Incomplete fermentation of extracts by selected yeasts allows the production of inulin with increased fructose content. The yeast strains (Saccharomyces cerevisiae, S. diastaticus...) are chosen for their ability to ferment sucrose and inulin small polymers, but not easily inulin large polymers. A good increase in the fructose ratio and a good yield in residual sugars can be obtained with the better strains. After fermentation and acid or enzymatic hydrolysis, extracts from “early” and “late” harvested tubers lead to syrups of good quality containing up to 95% and 90% of fructose respectively. This fermentative enrichment process is competitive with others (for example, chromatographic enrichment), is appropriate to raw extracts, simplifies the purification steps, and also permits the simultaneous benefit of production of by-products in the form of ethanol and yeast (in addition to the pulps). Unhydrolyzed inulin polymers with high fructose content can be recovered by this selective fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369858
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    Paper (German National Licenses)
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