ISSN:
1573-6849
Keywords:
DNA methylation
;
dosage compensation
;
escape from X inactivation
;
fluorescence in situ hybridization
;
transgenic animals
;
X-chromosome inactivation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We analyzed an X-linked metallothionein–vasopressin (MTVP) fusion transgene that undergoes X-chromosome inactivation (X inactiv ation) and an X-linked transferrin (TFN) transgene that escapes X inactivation with respect to methylation in the 5′ regulatory regions. The MTVP transgene promoter region is unmethylated when the transgene is on the active X chromosome and methylated when on the inactive X chromosome. Interestingly, the MTVP transgene is not detectably transcribed from the male X chromosome, although it is unmethylated, consistent with its availability for transcription. The TFN transgene promoter region is hypomethylated on both the active and inactive X chromosomes, consistent with its expression from both chromosomes. The TFN and MTVP transgenes have been mapped to chromosomal regions D and C, respectively, by fluorescence in situ hybridization. These observations are discussed in the context of our understanding of the role of DNA methylation in the spread and maintenance of X-chromosome inactivation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009229423535
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